{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T04:13:20Z","timestamp":1777349600093,"version":"3.51.4"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"08","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Endocrinol Diabetes"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Graves\u2019 ophthalmopathy is characterized by hyperthyroidism, which is associated with higher serum T3 levels than T4 due to deiodinase enzymes.<\/jats:p><jats:p>The effect of Graves\u2019 patient\u2019s sera (n=52) with elevated thyroid hormone and TSH receptor or thyroid peroxidase antibody (anti-TPO) levels was investigated on thyroidal, skeletal and eye muscle type 2 deiodinase enzyme (DII) activities. DII activities were measured with 125I-T4 substrate, while thyroid hormone and antibody levels with immunoassays.<\/jats:p><jats:p>In Graves\u2019 ophthalmopathy, sera with elevated FT4 or FT3 levels reduced DII activites remarkably in all tissue fractions. Thyroidal DII activities were lower than those using eye muscle fraction (0.6\u00b10.22 vs 1.14\u00b10.43\u2009pmol\/mg\/min, P&lt;0.006). Effect of sera with increased FT3 levels demonstrated also reduced DII activities in patients with Graves\u2019 ophthalmopathy after methimazole therapy compared to those who had no ophthalmopathy (2.88\u00b12 vs 20.42\u00b111.82\u2009pmol\/mg\/min, P&lt;0.006 for thyroidal fraction, 4.07\u00b12.72 vs 29.22\u00b115.46\u2009pmol\/mg\/min, P&lt;0.004 for skeletal muscle, 5.3\u00b13.47 vs 37.87\u00b118.82\u2009pmol\/mg\/min, P&lt;0.003 for eye muscle). Hyperthyroid sera with TSH receptor antibodies resulted in increased DII activities, while sera with anti-TPO antibodies were connected to lower DII activities in Graves\u2019 ophthalmopathy.<\/jats:p><jats:p>In summary, the actions of hyperthyroid sera derived from patients with Graves\u2019 disease were tested on tissue-specific DII activities. Elevated FT4 level-induced DII inactivation is present in Graves\u2019 ophthalmopathy, which seems to be also present at the beginning of methimazole therapy. Stimulating TSH receptor antibiodies increased DII activities via their nongenomic effects using sera of hyperthyroid Graves\u2019 ophthalmopathy, but anti-TPO antibodies could influence DII activities via altering FT4 levels.<\/jats:p>","DOI":"10.1055\/s-0043-113831","type":"journal-article","created":{"date-parts":[[2017,7,27]],"date-time":"2017-07-27T18:41:14Z","timestamp":1501180874000},"page":"514-521","source":"Crossref","is-referenced-by-count":4,"title":["Hyperthyroidism in Patients with Graves\u2019 Ophthalmopathy, and Thyroidal, Skeletal and Eye Muscle Specific Type 2 Deiodinase Enzyme Activities"],"prefix":"10.1055","volume":"125","author":[{"given":"Ildik\u00f3","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"Immunoendocrinology and Osteoporosis Centre, EndoMed, Bem t\u00e9r 18\/C., Debrecen,  Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zsef","family":"Szentmikl\u00f3si","sequence":"additional","affiliation":[{"name":"University of Debrecen, Department of Pharmacology and Pharmacotherapy, POBox 12, Debrecen,  Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9va","family":"Somogyin\u00e9-V\u00e1ri","sequence":"additional","affiliation":[{"name":"Immunoendocrinology and Osteoporosis Centre, EndoMed, Bem t\u00e9r 18\/C., Debrecen,  Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,7,27]]},"container-title":["Experimental and Clinical Endocrinology &amp; Diabetes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0043-113831.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,12,19]],"date-time":"2017-12-19T07:02:04Z","timestamp":1513666924000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0043-113831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,27]]},"references-count":0,"journal-issue":{"issue":"08","published-online":{"date-parts":[[2017,9,19]]},"published-print":{"date-parts":[[2017,9]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0043-113831","relation":{},"ISSN":["0947-7349","1439-3646"],"issn-type":[{"value":"0947-7349","type":"print"},{"value":"1439-3646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,27]]}}}