{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T12:24:46Z","timestamp":1753273486974},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Res (Stuttg)"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\nBackground Diazepam, as one of the most frequent prescribed drug from 1,4-benzodiazepine group, has certain limitations in pharmaceutical technology due to its poor solubility in water. By forming inclusion complexes with 2-hydroxypropyl-\u03b2-cyclodextrin, diazepam's biopharmaceutical properties can be greatly improved.<\/jats:p><jats:p>\nAim Aim of this research was to in vitro evaluate genotoxicity of prepared novel complexes of diazepam and their influence on proliferation of human peripheral blood lymphocytes.<\/jats:p><jats:p>\nMethods For identification of possible genotoxicity of diazepam inclusion complexes, cytokinesis-block micronucleus assay has been chosen. Evaluated concentrations of two diazepam inclusion complexes were 0.2\u2009\u00b5g\/mL, 0.5\u2009\u00b5g\/mL and 1.0\u2009\u00b5g\/mL in cell culture. For a reference, in vitro cytogenotoxicity evaluation of diazepam alone has been conducted as well.<\/jats:p><jats:p>\nResults Neither one of the diazepam, complexed nor non-complexed, in given concentrations showed genotoxicity, induced genetic damage or loss of genetic material.<\/jats:p><jats:p>\nConclusions Nuclear division index values, as indicators of cytostaticity and cytotoxicity suggested that investigated inclusion diazepam complexes induced accelerated proliferation of human peripheral blood lymphocytes in vitro, therefore possibly shortening the duration and dynamics of the cell cycle.<\/jats:p>","DOI":"10.1055\/s-0043-115123","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T18:51:58Z","timestamp":1500490318000},"page":"661-672","source":"Crossref","is-referenced-by-count":2,"title":["Cytogenotoxicity of Inclusion Complexes of Diazepam with 2-Hydroxypropyl-\u03b2-cyclodextrin"],"prefix":"10.1055","volume":"67","author":[{"given":"Jasmina","family":"Had\u017eiabdi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nevenka","family":"Kopjar","sequence":"additional","affiliation":[{"name":"Institute for Medical Research and Occupational Health, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davor","family":"\u017delje\u017ei\u0107","sequence":"additional","affiliation":[{"name":"Institute for Medical Research and Occupational Health, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selma","family":"\u0160pirtovi\u0107-Halilovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davorka","family":"Zavr\u0161nik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,7,19]]},"container-title":["Drug Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0043-115123.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,12,19]],"date-time":"2017-12-19T09:30:07Z","timestamp":1513675807000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0043-115123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,19]]},"references-count":0,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,10,26]]},"published-print":{"date-parts":[[2017,11]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0043-115123","relation":{},"ISSN":["2194-9379","2194-9387"],"issn-type":[{"value":"2194-9379","type":"print"},{"value":"2194-9387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,19]]}}}