{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T06:05:39Z","timestamp":1760853939114},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gamma irradiation as a decontaminating physical agent could be an important tool in the production chain of herbal medicines by improving the microbiological quality of raw materials and the safety of final products. This study was undertaken to investigate the genotoxic potential and eventual chemical modifications of a batch of Mikania glomerata raw material decontaminated by different doses of gamma irradiation (2.0, 3.5, and 5.0\u2009kGy), using a cesium-137 source. DNA damage was assessed in vitro by agarose gel electrophoresis in regard to double-chain breaks of plasmid pUC 9.1 DNA and in vivo by micronucleus test in bone marrow cells of Wistar rats. Cytotoxicity in bone marrows was assessed by scoring polychromatic and normochromatic erythrocytes ratio. An HPLC-DAD method was adapted and validated for the enhancement of coumarin selectivity from the other matrix constituents. The microbial load was satisfactorily reduced, leading to sterilization at the highest dose. Genotoxic and cytotoxic effects were not increased in the in vitro and in vivo models. The concentration of coumarin and the chromatographic profiles of the hydroalcoholic plant extracts (ethanol 70% v\/v) were not modified after such process. Therefore, this work suggests that gamma irradiation of M.\u00a0glomerata raw material is suitable and safe for microbiological control purposes at the employed doses.<\/jats:p>","DOI":"10.1055\/s-0043-115224","type":"journal-article","created":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T22:46:45Z","timestamp":1499899605000},"page":"65-72","source":"Crossref","is-referenced-by-count":1,"title":["Decontamination of Mikania glomerata Leaves by Gamma Irradiation: Coumarin Determination by HPLC-DAD, Microbiological Control and Genotoxicological Studies"],"prefix":"10.1055","volume":"84","author":[{"given":"Marcos","family":"Gouv\u00eaa","sequence":"additional","affiliation":[{"name":"Pr\u00f3-Reitoria de Extens\u00e3o, LURA, Universidade Federal Fluminense, Niter\u00f3i, Brazil"},{"name":"Instituto Vital Brazil, LDNF, Niter\u00f3i, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Qu\u00edmica, Instituto de Qu\u00edmica, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samara","family":"Ferreira-Machado","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Roberto Alc\u00e2ntara Gomes, LCR, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil"},{"name":"Departamento de Biologia Geral, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thalita","family":"da Silva","sequence":"additional","affiliation":[{"name":"Pr\u00f3-Reitoria de Extens\u00e3o, LURA, Universidade Federal Fluminense, Niter\u00f3i, Brazil"},{"name":"Instituto Vital Brazil, LDNF, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Lima","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Roberto Alc\u00e2ntara Gomes, LCR, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio","family":"Lau","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Roberto Alc\u00e2ntara Gomes, LCR, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana","family":"Esper","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Aplicadas a Produtos da Sa\u00fade, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Niter\u00f3i, Brazil"},{"name":"Departamento de Bromatologia, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samanta","family":"Mour\u00e3o","sequence":"additional","affiliation":[{"name":"Pr\u00f3-Reitoria de Extens\u00e3o, LURA, Universidade Federal Fluminense, Niter\u00f3i, Brazil"},{"name":"Instituto Vital Brazil, LDNF, 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Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edson","family":"Andrade","sequence":"additional","affiliation":[{"name":"Centro Tecnol\u00f3gico do Ex\u00e9rcito, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annibal","family":"Netto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Qu\u00edmica, Instituto de Qu\u00edmica, Universidade Federal Fluminense, Niter\u00f3i, Brazil"},{"name":"Departamento de Qu\u00edmica Anal\u00edtica, LaQAFA, Instituto de Qu\u00edmica, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via","family":"Marques","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Qu\u00edmica, Instituto de Qu\u00edmica, Universidade Federal Fluminense, Niter\u00f3i, Brazil"},{"name":"Departamento de Qu\u00edmica Anal\u00edtica, LaQAFA, Instituto de Qu\u00edmica, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Peregrino","sequence":"additional","affiliation":[{"name":"Pr\u00f3-Reitoria de Extens\u00e3o, LURA, Universidade Federal Fluminense, Niter\u00f3i, Brazil"},{"name":"Instituto Vital Brazil, LDNF, Niter\u00f3i, Brazil"},{"name":"Departamento de Tecnologia Farmac\u00eautica, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,7,12]]},"container-title":["Planta 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