{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:05:27Z","timestamp":1773381927272,"version":"3.50.1"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"14\/15","license":[{"start":{"date-parts":[[2017,9,8]],"date-time":"2017-09-08T00:00:00Z","timestamp":1504828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The roots of Bupleurum chinense have a long history in traditional medicine to treat infectious diseases and inflammatory disorders. Two major compounds, saikosaponins A and D, were reported to exert potent anti-inflammatory activity by inhibiting NF-\u03baB. In the present study, we isolated new saikosaponin analogues from the roots of B. chinese interfering with NF-\u03baB activity in vitro. The methanol-soluble fraction of the dichloromethane extract of Radix Bupleuri was subjected to activity-guided isolation yielding 18 compounds, including triterpenoids and polyacetylenes. Their structures were determined by spectroscopic methods as saikogenin D (1), prosaikogenin D (2), saikosaponins B2 (3), W (4), B1 (5), Y (6), D (7), A (8), E (9), B4 (10), B3 (11), and T (12), saikodiyne A (13), D (14), E (15) and F (16), falcarindiol (17), and 1-linoleoyl-sn-glycero-3-phosphorylcholine (18). Among them, 4, 15, and 16 are new compounds, whereas 6, previously described as a semi-synthetic compound, is isolated from a natural source for the first time, and 13\u201317 are the first reports of polyacetylenes from this plant. Nine saponins\/triterpenoids were tested for inhibition of NF-\u03baB signaling in a cell-based NF-\u03baB-dependent luciferase reporter gene model in vitro. Five of them (1, 2, 4, 6, and 8) showed strong (&gt;\u200950%, at 30\u2009\u00b5M) NF-\u03baB inhibition, but also varying degrees of cytotoxicity, with compounds 1 and 4 (showing no significant cytotoxicity) presenting IC50 values of 14.0\u2009\u00b5M and 14.1\u2009\u00b5M in the cell-based assay, respectively.<\/jats:p>","DOI":"10.1055\/s-0043-118226","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T13:15:13Z","timestamp":1505308513000},"page":"1242-1250","source":"Crossref","is-referenced-by-count":16,"title":["Bupleurum chinense Roots: a Bioactivity-Guided Approach toward Saponin-Type NF-\u03baB Inhibitors"],"prefix":"10.1055","volume":"83","author":[{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Latkolik","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, University of Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atanas","family":"Atanasov","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, University of Vienna, Austria"},{"name":"Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaf","family":"Kunert","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Austria;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva-Maria","family":"Pferschy-Wenzig","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elke","family":"Heiss","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, University of Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemens","family":"Malainer","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, University of Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Schinkovitz","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Kollroser","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine, Medical University of Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Dirsch","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, University of Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"Bauer","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,9,8]]},"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0043-118226.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T19:29:26Z","timestamp":1557170966000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0043-118226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,8]]},"references-count":0,"journal-issue":{"issue":"14\/15","published-online":{"date-parts":[[2017,9,27]]},"published-print":{"date-parts":[[2017,10]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0043-118226","relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,8]]}}}