{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T17:48:35Z","timestamp":1793036915939,"version":"4.5.0"},"reference-count":26,"publisher":"Scientific Scholar","issue":"03","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background\u2003Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created high demand for molecular kits and consumables for mass screening of suspected individuals. Direct real-time polymerase chain reaction (RT-PCR) assay without nucleic acid extraction has several advantages in saving testing time and cost and helps in the rapid reporting of SARS-CoV-2. The present study evaluated the analytical performance of four SARS-CoV-2 RT-PCR for direct RT-PCR testing using preheated specimens.<\/jats:p><jats:p>\n          Methods\u2003A total of 100 clinical specimens were selected and divided into three different groups: (1) group I: 20 SARS-CoV-2 positive specimens with high viral load, viz., low Ct values (&lt; 30 Ct), (2) group II: 50 SARS-CoV-2 positive specimens with low viral load, viz., high Ct values (&gt; 30 Ct), and (3) group III: 30 SARS-CoV-2 negative specimens. Specimens were heat-inactivated at 70\u00b0C for 10\u2009minutes and cooled down at 4\u00b0C and were evaluated for standard and direct RT-PCR method by using ViralDtect-II Multiplex Real-Time PCR kit, TaqPath COVID-19 Combo kit, COVIDsure Pro Multiplex RT-PCR kit, and Hi-PCR Coronavirus (COVID-19) Multiplex Probe PCR kit.<\/jats:p><jats:p>\n          Results\u2003Results showed that except ViralDtect-II kit, the other three TaqPath COVID-19 Combo kit, COVIDsure Pro kit, and Hi-PCR Coronavirus (COVID-19) RT-PCR kit were able to amplify all the SARS-CoV-2 genes in the direct RT-PCR method using preheated specimens. In group I specimens, 100% sensitivity was observed in all three RT-PCR kits. In group II specimens, COVIDsure Pro kit was found to be superior among other kits.<\/jats:p><jats:p>\n          Conclusion\u2003Direct RT-PCR method during pandemic situation is valuable and cost effective for the detection of SARS-CoV-2. All three TaqPath COVID-19 Combo kit, COVIDsure Pro kit, and Hi-PCR Coronavirus (COVID-19) RT-PCR kit can be used for direct RT-PCR method and COVIDsure Pro kit performance was found to be superior among all.<\/jats:p>","DOI":"10.1055\/s-0043-1760752","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T00:05:12Z","timestamp":1675123512000},"page":"383-391","source":"Crossref","is-referenced-by-count":3,"title":["Performance Evaluation of Different RT-PCR Kits for the Direct Detection of SARS-CoV-2 in Preheated Specimens"],"prefix":"10.25259","volume":"15","author":[{"given":"Rajeev Kumar","family":"Jain","sequence":"additional","affiliation":[{"name":"State Virology Laboratory, Gandhi Medical College, Bhopal, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3545-4171","authenticated-orcid":false,"given":"Nagaraj","family":"Perumal","sequence":"additional","affiliation":[{"name":"State Virology Laboratory, Gandhi Medical College, Bhopal, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepti","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gandhi Medical College, Bhopal, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh","family":"Shrivastava","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gandhi Medical College, Bhopal, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamlesh Kumar","family":"Ahirwar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gandhi Medical College, Bhopal, Madhya Pradesh, 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