{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T04:05:29Z","timestamp":1728878729745},"reference-count":27,"publisher":"Georg Thieme Verlag KG","issue":"04","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Objectives\u2003The purpose of the present study was to evaluate the efficacy of spiral ganglion neuron (SGN) regeneration after dental pulp stem cell (DPSC) transplantation in a rat sensorineural hearing loss (HL) model.<\/jats:p><jats:p>Materials and Methods\u2003Sham or experimental HL was induced in adult Sprague\u2013Dawley rats by cochlear round window surgery. An HL rat model was established with a single 10\u2009mM ouabain intratympanic injection. After 7 days, the rats received DPSCs, stem cells from human exfoliated deciduous teeth (SHED), or culture medium in the sutural area to establish four groups: sham, HL-DPSC, HL-SHED, and HL-medium. Histological analyses were performed at 4, 7, and 10 weeks after transplantation, and the number of SGNs, specific SGN protein expression, and the function of SGNs were evaluated.<\/jats:p><jats:p>Statistical Analysis\u2003Data were statistically by MS Excel and SPSS v.15.0. Intergroup level of significance was determined via a one-way analysis of variance and Duncan's multiple range test with 95% confidence intervals.<\/jats:p><jats:p>Results\u2003New SGN formation was observed in the HL-DPSC and HL-SHED rat groups. The number of SGNs was significantly higher in the HL-DPSC and HL-SHED groups than in the HL-medium group over 4 to 10-week survival period. HL-DPSC rats exhibited higher SGN density compared with that in HL-SHED group, which was statistically significant at week 10. The regenerated SGNs expressed cochlear wiring regulator GATA-binding-protein 3. Moreover, the SGNs from the HL-DPSC group also exhibited a higher expression of synaptic vesicle protein and regulated action potential-dependent neurotransmitter release compared with SGNs from the HL-SHED group.<\/jats:p><jats:p>Conclusions\u2003Our findings suggest that DPSCs and SHED repair and regenerate SGNs in rat HL model. Dental pulp stem cells represent a promising treatment strategy for restoring damage to the sensory circuits associated with deafness.<\/jats:p>","DOI":"10.1055\/s-0043-1761190","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T00:02:42Z","timestamp":1675123362000},"page":"1207-1214","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of the Efficacy of Human Dental Pulp Stem Cell Transplantation in Sprague\u2013Dawley Rats with Sensorial Neural Hearing Loss"],"prefix":"10.1055","volume":"17","author":[{"given":"Visut","family":"Rawiwet","sequence":"additional","affiliation":[{"name":"Central Animal Facility, Faculty of Science, Mahidol University (MUSC-CAF), Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rattanavijit","family":"Vijitruth","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chareonsri","family":"Thonabulsombat","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kutkao","family":"Vongsavan","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hathaitip","family":"Sritanaudomchai","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,1,30]]},"reference":[{"issue":"01","key":"ref1","first-page":"e1","article-title":"The lived experiences and psychosocial impact of hearing loss on the quality of life of adults with multidrug-resistant tuberculosis","volume":"69","author":"A B Thusi","year":"2022","journal-title":"S Afr J Commun Disord"},{"issue":"08","key":"ref2","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/j.ijporl.2008.04.004","article-title":"Auditory neuropathy: endocochlear lesion or temporal processing impairment? 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