{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T20:33:33Z","timestamp":1789763613751,"version":"4.0.1"},"reference-count":36,"publisher":"Georg Thieme Verlag KG","issue":"02","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003Clinical methods use the subjective diagnosis of periodontal diseases by visual observation that could result in differences and variability of diagnosis. The addition of specific markers could aid in the accurate diagnosis of the local population. The objective of the study was to target two of the major proteins for possible significance in such an approach.<\/jats:p><jats:p>\n          Materials and Methods\u2003Unstimulated saliva samples were collected from 60 participants aged between 18 and 70 years. Three groups each with twenty participants were recruited into periodontitis, gingivitis, and healthy control.<\/jats:p><jats:p>\n          Statistical Analysis\u2003The samples were analyzed using human enzyme-linked immunosorbent assay kits for matrix metalloproteinase-8 (MMP-8) and interleukin-1\u03b2 (IL-1\u03b2).<\/jats:p><jats:p>\n          Results\u2003SPSS version 20 was used to analyze the result. Posthoc analysis by Tukey's test revealed that MMP-8 levels were higher in gingivitis and periodontitis groups as compared with healthy controls. The test also revealed that IL-1\u03b2 levels were higher in the periodontitis group compared with the healthy control and gingivitis group. Additionally, one-way analysis of variance analysis showed a significant effect on probing depth in gingivitis and periodontitis patients. The mean age of periodontitis group was significantly higher than other groups.<\/jats:p><jats:p>\n          Conclusion\u2003Salivary biomarkers may provide useful diagnostic information and could be utilized as tests for periodontal disease screening, prognosis, and prediction.<\/jats:p>","DOI":"10.1055\/s-0043-1772779","type":"journal-article","created":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T00:07:18Z","timestamp":1702426038000},"page":"672-679","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of Salivary MMP-8 and IL-1\u03b2 for the Diagnosis of Periodontal Diseases in Pakistani Population"],"prefix":"10.1055","volume":"18","author":[{"given":"Rida","family":"Kainat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Baqai Medical University, Karachi, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iftikhar","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Baqai Medical University, Karachi, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah Mohammed","family":"Alolaywi","sequence":"additional","affiliation":[{"name":"Dental Department, Hotat Bani Tamim General Hospital, Ministry of Health, Riyad, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humera","family":"Waheed","sequence":"additional","affiliation":[{"name":"Dow College of Biotechnology, Dow University of Health Sciences, Karachi, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7998-7335","authenticated-orcid":false,"given":"Zohaib Khurshid","family":"Sultan","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Dental Implantology, College of Dentistry, King Faisal University, Al-Ahsa, Saudia Arabia"},{"name":"Department of Anatomy, Faculty of Dentistry, Center of Excellence for Regenerative Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6058-623X","authenticated-orcid":false,"given":"Syed Faraz","family":"Moin","sequence":"additional","affiliation":[{"name":"Dr. Zafar H. 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Leah","year":"2021"},{"issue":"03","key":"ref7","doi-asserted-by":"crossref","first-page":"385","DOI":"10.4103\/ejd.ejd_183_17","article-title":"Human salivary protein extraction from RNAPro\u00b7SAL\u2122, Pure\u00b7SAL\u2122, and passive drooling method","volume":"11","author":"Z Khurshid","year":"2017","journal-title":"Eur J Dent"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/bs.acc.2020.04.005","article-title":"Biochemical analysis of oral fluids for disease detection","volume":"100","author":"Z Khurshid","year":"2021","journal-title":"Adv Clin Chem"},{"issue":"01","key":"ref9","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1039\/C7AN01571B","article-title":"Chemical analysis in saliva and the search for salivary biomarkers - a tutorial review","volume":"143","author":"K Ngamchuea","year":"2017","journal-title":"Analyst 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Gasner","year":"2022"},{"issue":"01","key":"ref14","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1600-0757.2008.00288.x","article-title":"Saliva as a diagnostic tool for periodontal disease: current state and future directions","volume":"50","author":"W V Giannobile","year":"2009","journal-title":"Periodontol 2000"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3389\/fcimb.2015.00062","article-title":"Targeted salivary biomarkers for discrimination of periodontal health and disease(s)","volume":"5","author":"J L Ebersole","year":"2015","journal-title":"Front Cell Infect Microbiol"},{"issue":"06","key":"ref16","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.3390\/jcm9061720","article-title":"Oral fluid biomarkers for diagnosing gingivitis in human: a cross-sectional study","volume":"9","author":"I Hong","year":"2020","journal-title":"J Clin 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Res"},{"issue":"08","key":"ref20","first-page":"2676","article-title":"Comparative assessment of periodontal treatment needs among the electronic cigarette users and traditional smokers","volume":"26","author":"A S Alqahtani","year":"2022","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"35S","DOI":"10.14219\/jada.archive.2008.0353","article-title":"Measuring salivary flow: challenges and opportunities","volume":"139","author":"M Navazesh","year":"2008","journal-title":"J Am Dent Assoc"},{"issue":"06","key":"ref22","doi-asserted-by":"crossref","first-page":"846","DOI":"10.3390\/ijms17060846","article-title":"Human saliva collection devices for proteomics: an update","volume":"17","author":"Z Khurshid","year":"2016","journal-title":"Int J Mol Sci"},{"issue":"19","key":"ref23","doi-asserted-by":"crossref","first-page":"3677","DOI":"10.1002\/jssc.202100384","article-title":"Saliva, a bodily fluid with recognized and potential 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Mdala","year":"2014","journal-title":"J Clin Periodontol"},{"issue":"06","key":"ref27","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1111\/j.1600-0765.2007.00980.x","article-title":"Gingival inflammation, increased periodontal pocket depth and elevated interleukin-6 in gingival crevicular fluid of depressed women on long-term sick leave","volume":"42","author":"A Johannsen","year":"2007","journal-title":"J Periodontal Res"},{"issue":"04","key":"ref28","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.archoralbio.2007.11.005","article-title":"Correlation between salivary IL-1\u03b2 levels and periodontal clinical status","volume":"53","author":"S I Tob\u00f3n-Arroyave","year":"2008","journal-title":"Arch Oral Biol"},{"issue":"01","key":"ref29","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s10266-019-00435-5","article-title":"Relationship between TNF-\u03b1, MMP-8, and MMP-9 levels in gingival crevicular fluid and the subgingival microbiota in 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