{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:06:51Z","timestamp":1789693611997,"version":"4.0.1"},"reference-count":77,"publisher":"Georg Thieme Verlag KG","issue":"11","license":[{"start":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:00:00Z","timestamp":1697414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["1123"],"award-info":[{"award-number":["1123"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["914"],"award-info":[{"award-number":["914"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010578","name":"Deutsche Gesellschaft f\u00fcr Kardiologie-Herz und Kreislaufforschung","doi-asserted-by":"crossref","award":["DGK09\/2020"],"award-info":[{"award-number":["DGK09\/2020"]}],"id":[{"id":"10.13039\/501100010578","id-type":"DOI","asserted-by":"crossref"}]},{"name":"ERC starting grant","award":["947611"],"award-info":[{"award-number":["947611"]}]},{"name":"F\u00f6rderprogramm f\u00fcr Forschung und Lehre","award":["1123"],"award-info":[{"award-number":["1123"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>\n            Background\u2003Deep vein thrombosis (DVT) is a common condition associated with significant mortality due to pulmonary embolism. Despite advanced prevention and anticoagulation therapy, the incidence of venous thromboembolism remains unchanged. Individuals with elevated hematocrit and\/or excessively high erythropoietin (EPO) serum levels are particularly susceptible to DVT formation. We investigated the influence of short-term EPO administration compared to chronic EPO overproduction on DVT development. Additionally, we examined the role of the spleen in this context and assessed its impact on thrombus composition.<\/jats:p><jats:p>\n            Methods\u2003We induced ligation of the caudal vena cava (VCC) in EPO-overproducing Tg(EPO) mice as well as wildtype mice treated with EPO for two weeks, both with and without splenectomy. The effect on platelet circulation time was evaluated through FACS analysis, and thrombus composition was analyzed using immunohistology.<\/jats:p><jats:p>\n            Results\u2003We present evidence for an elevated thrombogenic phenotype resulting from chronic EPO overproduction, achieved by combining an EPO-overexpressing mouse model with experimental DVT induction. This increased thrombotic state is largely independent of traditional contributors to DVT, such as neutrophils and platelets. Notably, the pronounced prothrombotic effect of red blood cells (RBCs) only manifests during chronic EPO overproduction and is not influenced by splenic RBC clearance, as demonstrated by splenectomy. In contrast, short-term EPO treatment does not induce thrombogenesis in mice. Consequently, our findings support the existence of a differential thrombogenic effect between chronic enhanced erythropoiesis and exogenous EPO administration.<\/jats:p><jats:p>\n            Conclusion\u2003Chronic EPO overproduction significantly increases the risk of DVT, while short-term EPO treatment does not. These findings underscore the importance of considering EPO-related factors in DVT risk assessment and potential therapeutic strategies.<\/jats:p>","DOI":"10.1055\/s-0043-1775965","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T07:43:30Z","timestamp":1697528610000},"page":"1027-1039","source":"Crossref","is-referenced-by-count":6,"title":["Differential Effects of Erythropoietin Administration and Overexpression on Venous Thrombosis in Mice"],"prefix":"10.1055","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6555-6622","authenticated-orcid":false,"given":"Sven","family":"Stockhausen","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badr","family":"Kilani","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Schubert","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Lena","family":"Steinsiek","sequence":"additional","affiliation":[{"name":"Department of cardiology, German Heart Center, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Chandraratne","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Wendler","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Eivers","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Luise","family":"von Br\u00fchl","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Massberg","sequence":"additional","affiliation":[{"name":"Medizinische Klinik und Poliklinik I, Klinikum der Universit\u00e4t M\u00fcnchen, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"},{"name":"German Center for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany"},{"name":"Walter-Brendel Center of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilka","family":"Ott","sequence":"additional","affiliation":[{"name":"Department of cardiology, German Heart Center, Munich, 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