{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T02:14:06Z","timestamp":1780971246028,"version":"3.54.1"},"reference-count":34,"publisher":"Georg Thieme Verlag KG","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropediatrics"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background\u2003Neonatal hypoxic-ischemic brain injury (HIBI) results from disruptions to blood supply and oxygen in the perinatal brain. The goal of this study was to measure brain sterol metabolites and plasma oxysterols after injury in a neonatal HIBI mouse model to assess for potential therapeutic targets in the brain biochemistry as well as potential circulating diagnostic biomarkers.<\/jats:p><jats:p>\n          Methods\u2003Postnatal day 9 CD1-IGS mouse pups were randomized to HIBI induced by carotid artery ligation followed by 30\u2009minutes at 8% oxygen or to sham surgery and normoxia. Brain tissue was collected for sterol analysis by liquid chromatography with tandem mass spectrometry (LC\u2013MS\/MS). Plasma was collected for oxysterol analysis by LC\u2013MS\/MS.<\/jats:p><jats:p>\n          Results\u2003There were minimal changes in brain sterol concentrations in the first 72\u2009hours after HIBI. In severely injured brains, there was a significant increase in desmosterol, 7-DHC, 8-DHC, and cholesterol 24\u2009hours after injury in the ipsilateral tissue. Lanosterol, 24-dehydrolathosterol, and 14-dehydrozymostenol decreased in plasma 24\u2009hours after injury. Severe neonatal HIBI was associated with increased cholesterol and sterol precursors in the cortex at 24\u2009hours after injury.<\/jats:p><jats:p>\n          Conclusions\u2003Differences in plasma oxysterols were seen at 24\u2009hours but were not present at 30\u2009minutes after injury, suggesting that these sterol intermediates would be of little value as early diagnostic biomarkers.<\/jats:p>","DOI":"10.1055\/s-0043-1776286","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T22:59:31Z","timestamp":1698101971000},"page":"023-031","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Neonatal Hypoxic-Ischemic Injury on Brain Sterol Synthesis and Metabolism"],"prefix":"10.1055","volume":"55","author":[{"given":"Amanda M.","family":"Dave","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Nebraska Medical Center, Omaha, Nebraska, United States"},{"name":"Department of Pediatrics, Children's Hospital and Medical Center, Omaha, Nebraska, United States"},{"name":"Child Health Research Institute, Omaha, Nebraska, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ned A.","family":"Porter","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Vanderbilt University, Nashville, Tennessee, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeljka","family":"Korade","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Nebraska Medical Center, Omaha, Nebraska, United States"},{"name":"Child Health Research Institute, Omaha, Nebraska, United States"},{"name":"Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Peeples","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Nebraska Medical Center, Omaha, Nebraska, United States"},{"name":"Department of Pediatrics, Children's Hospital and Medical Center, Omaha, Nebraska, United States"},{"name":"Child Health Research Institute, Omaha, Nebraska, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2023,10,23]]},"reference":[{"issue":"06","key":"ref1","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.earlhumdev.2010.05.010","article-title":"Epidemiology of neonatal encephalopathy and hypoxic-ischaemic encephalopathy","volume":"86","author":"J J Kurinczuk","year":"2010","journal-title":"Early Hum Dev"},{"issue":"03","key":"ref2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1053\/j.nainr.2011.07.004","article-title":"Hypoxic ischemic encephalopathy: pathophysiology and experimental treatments","volume":"11","author":"K A Allen","year":"2011","journal-title":"Newborn Infant Nurs 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