{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T14:23:35Z","timestamp":1775226215897,"version":"3.50.1"},"reference-count":23,"publisher":"Georg Thieme Verlag KG","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Semin Thromb Hemost"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This is a celebratory reprint of a historical paper published in STH in 1998. The original Abstract follows.<\/jats:p><jats:p>The PFA-100 system is a platelet function analyzer designed to measure platelet-related primary hemostasis. The instrument uses two disposable cartridges: a collagen\/epinephrine (CEPI) and a collagen\/ADP (CADP) cartridge. Previous experience has shown that CEPI cartridges detect qualitative platelet defects, including acetylsalicylic acid (ASA)-induced abnormalities, while CADP cartridges detect only thrombocytopathies and not ASA use. In this seven-center trial, 206 healthy subjects and 176 persons with various platelet-related defects, including 127 ASA users, were studied. The platelet function status was determined by a platelet function test panel. Comparisons were made as to how well the defects were identified by the PFA-100 system and by platelet aggregometry. The reference intervals for both cartridges, testing the 206 healthy subjects, were similar to values described in smaller studies in the literature (mean closure time [CT] of 132\u2009seconds for CEPI and 93\u2009seconds for CADP). The use of different lot numbers of cartridges or duplicate versus singleton testing revealed no differences. Compared with the platelet function status, the PFA-100 system had a clinical sensitivity of 94.9% and a specificity of 88.8%. For aggregometry, a sensitivity of 94.3% and a specificity of 88.3% were obtained. These values are based on all 382 specimens. A separate analysis of sensitivity by type of platelet defect, ASA use versus congenital thrombocytopathies, revealed for the PFA-100 system a 94.5% sensitivity in identifying ASA users and a 95.9% sensitivity in identifying the other defects. For aggregometry, the values were 100% for ASA users and 79.6% for congenital defects. Analysis of concordance between the PFA-100 system and aggregometry revealed no difference in clinical sensitivity and specificity between the systems (p\u2009&gt;\u20090.9999). The overall agreement was 87.5%, with a Kappa index of 0.751. The two tests are thus equivalent in their ability to identify normal and abnormal platelet defects. Testing 126 subjects who took 325\u2009mg ASA revealed that the PFA-100 system (CEPI) was able to detect 71.7% of ASA-induced defects with a positive predictive value of 97.8%. The overall clinical accuracy of the system, calculated from the area under the receiver operating characteristic curve, was 0.977. The data suggest that the PFA-100 system is highly accurate in discriminating normal from abnormal platelet function. The ease of operation of the instrument makes it a useful tool to use in screening patients for platelet-related hemostasis defects.<\/jats:p>","DOI":"10.1055\/s-0043-1777306","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:02:17Z","timestamp":1702512137000},"page":"664-671","source":"Crossref","is-referenced-by-count":5,"title":["PFA-100 System: A New Method for Assessment of Platelet Dysfunction"],"prefix":"10.1055","volume":"50","author":[{"given":"Eberhard F.","family":"Mammen","sequence":"additional","affiliation":[{"name":"Wayne State University School of Medicine, Detroit, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip C.","family":"Comp","sequence":"additional","affiliation":[{"name":"University of Oklahoma School of Medicine, Oklahoma City, Oklahoma"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Gosselin","sequence":"additional","affiliation":[{"name":"University of California Davis Medical Center, Sacramento, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Greenberg","sequence":"additional","affiliation":[{"name":"Duke University Medical Center, Durham, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Keith","family":"Hoots","sequence":"additional","affiliation":[{"name":"University of Texas, Houston, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig M.","family":"Kessler","sequence":"additional","affiliation":[{"name":"Georgetown University Medical Center, Washington, District of Columbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward C.","family":"Larkin","sequence":"additional","affiliation":[{"name":"East Carolina University School of Medicine, Greenville, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darla","family":"Liles","sequence":"additional","affiliation":[{"name":"University of California Davis Medical Center, Sacramento, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane J.","family":"Nugent","sequence":"additional","affiliation":[{"name":"Children's Hospital of Orange County, Orange County, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,12,13]]},"reference":[{"key":"ref1","first-page":"753","volume-title":"Platelets","author":"P W Majerus","year":"1994"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1146\/annurev.cb.06.110190.001553","article-title":"Platelet membrane glycoproteins: functions in cellular interactions","volume":"6","author":"N Kieffer","year":"1990","journal-title":"Annu Rev Cell Biol"},{"issue":"20","key":"ref3","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1056\/NEJM197905173002006","article-title":"Arachidonic acid metabolites and the interactions between platelets and blood-vessel walls","volume":"300","author":"S Moncada","year":"1979","journal-title":"N Engl J Med"},{"key":"ref4","first-page":"2l0","article-title":"Measurement of the bleeding time","volume":"52","author":"C H Mielke","year":"1984","journal-title":"Thromb 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02","key":"ref12","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1055\/s-0032-1313613","article-title":"Preliminary data from a field trial of the PFA-100 system","volume":"21","author":"E F Mammen","year":"1995","journal-title":"Semin Thromb Hemost"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1055\/s-0032-1313612","article-title":"Description of an in vitro platelet function analyzer\u2013PFA-100","volume":"21","author":"S K Kundu","year":"1995","journal-title":"Semin Thromb Hemost"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1177\/107602969600200404","article-title":"Characterization of an in vitro platelet function analyzer, PFA-l00\u2122","volume":"2","author":"S Kundu","year":"1996","journal-title":"Clin Appl Thromb Hemost"},{"key":"ref17","first-page":"633","article-title":"In-vitro assessment of platelet inhibition with SC-54701 by PFA-100\u2122","volume":"78","author":"S Kundu","year":"1997","journal-title":"Thromb Haemost"},{"issue":"01","key":"ref18","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0049-3848(97)00115-1","article-title":"Comparison of four commercial citrate blood collection systems for platelet function analysis by the PFA-100 system","volume":"87","author":"E J Heilmann","year":"1997","journal-title":"Thromb Res"},{"issue":"02","key":"ref19","first-page":"117","article-title":"Characterization of preanalytical and technical variables when using the PFA-100\u2122 test system","volume":"74","author":"M Wilmer","year":"1997","journal-title":"Ann Hematol"},{"key":"ref20","first-page":"74","article-title":"Evaluation of platelet function in a pediatric setting using the platelet function analyzer (PFA-100\u2122)","volume":"78","author":"M D Carcao","year":"1997","journal-title":"Thromb Haemost"},{"key":"ref21","first-page":"696","article-title":"Screening and control of therapy of patients with von Willebrand disease using a new analyzer of platelet function under high shear stress","volume":"78","author":"E Fressinaud","year":"1997","journal-title":"Thromb Haemost"},{"issue":"S 02","key":"ref22","first-page":"118","article-title":"Platelet function analyzer (PFA-I00\u2122) for screening of von Willebrand's disease (vWD)","volume":"74","author":"M Weippart-Kretschmer","year":"1997","journal-title":"Ann Haematol"},{"issue":"02","key":"ref23","first-page":"72","article-title":"PFA-100\u2122 system: a sensitive screening test for platelet-related hemostasis defects","volume":"74","author":"C M Maier","year":"1997","journal-title":"Ann Hematol"},{"issue":"02","key":"ref24","first-page":"136","article-title":"Serial assessment of platelet function following a single 300\u2009mg dose of acetylsalicylic acid","volume":"74","author":"J Bohner","year":"1997","journal-title":"Ann Hematol"},{"issue":"02","key":"ref25","first-page":"73","article-title":"Measurement of the effect of aspirin and ticlopidine on platelet function with a simple in vitro bleeding test","volume":"74","author":"M Borris","year":"1997","journal-title":"Ann Hematol"}],"container-title":["Seminars in Thrombosis and 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