{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T12:12:34Z","timestamp":1793448754059,"version":"4.5.0"},"reference-count":7,"publisher":"Georg Thieme Verlag KG","issue":"02","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Semin Thromb Hemost"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This manuscript represents a republication of a manuscript originally published in STH in 1995. This republication is to help celebrate 50 years of publishing for STH. The original abstract follows.<\/jats:p><jats:p>A new in vitro system for the detection of platelet dysfunction, PFA-100\u00ae, has been developed. It provides a quantitative measure of platelet function in anticoagulated whole blood. The system comprises a microprocessor-controlled instrument and a disposable test cartridge containing a biologically active membrane. The instrument aspirates a blood sample under constant vacuum from the sample reservoir through a capillary and a microscopic aperture cut into the membrane. The membrane is coated with collagen and epinephrine or adenosine 5\u2032-diphosphate. The presence of these biochemical stimuli, and the high shear rates generated under the standardized flow conditions, result in platelet attachment, activation, and aggregation, slowly building a stable platelet plug at the aperture. The time required to obtain full occlusion of the aperture is reported as the \u201cclosure time.\u201d We have found that impairment of von Willebrand factor, or inhibition of platelet receptors glycoprotein Ib or IIb\/IIIa with monoclonal antibodies or peptides, resulted in abnormal closure times. An antifibrinogen antibody, in contrast, failed to show any effect. The test appears to be sensitive to platelet adherence and aggregation abnormalities. The PFA-100\u00ae system has potential applications in routine evaluation of platelet function in the clinical setting because of its accuracy, case of operation, and rapid turnaround of results.<\/jats:p>","DOI":"10.1055\/s-0043-1777308","type":"journal-article","created":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T00:05:28Z","timestamp":1702425928000},"page":"314-319","source":"Crossref","is-referenced-by-count":6,"title":["Description of an In Vitro Platelet Function Analyzer\u2014PFA-100\u2122"],"prefix":"10.1055","volume":"50","author":[{"given":"Sourav K.","family":"Kundu","sequence":"additional","affiliation":[{"name":"Dade International Inc., Miami, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Heilmann","sequence":"additional","affiliation":[{"name":"Dade International Inc., Miami, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reynaldo","family":"Sio","sequence":"additional","affiliation":[{"name":"Dade International Inc., Miami, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Garcia","sequence":"additional","affiliation":[{"name":"Dade International Inc., Miami, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa M.","family":"Davidson","sequence":"additional","affiliation":[{"name":"Dade International Inc., Miami, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy A.","family":"Ostgaard","sequence":"additional","affiliation":[{"name":"Dade International Inc., Miami, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2023,12,12]]},"reference":[{"issue":"06","key":"ref1","first-page":"357","article-title":"Simulation of primary haemostasis in vitro","volume":"15","author":"M AA Kratzer","year":"1985","journal-title":"Haemostasis"},{"key":"ref3","first-page":"317","article-title":"The in vitro bleeding test-standardization of the methodical procedure","volume":"17","author":"G Dietrich","year":"1993","journal-title":"Lab Med"},{"issue":"05","key":"ref4","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/0049-3848(89)90267-3","article-title":"In vitro bleeding test\u2013a simple method for the detection of aspirin effects on platelet function","volume":"56","author":"V Kretschmer","year":"1989","journal-title":"Thromb Res"},{"issue":"40","key":"ref5","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1093\/clinchem\/40.9.1827","article-title":"Evaluation of platelet function by PFA-100\u2122","author":"S R Kundu","year":"1994","journal-title":"Clin Chem"},{"issue":"06","key":"ref6","first-page":"363","article-title":"Detection of abnormal platelet functions with an in vitro model of primary haemostasis","volume":"15","author":"M AA Kratzer","year":"1985","journal-title":"Haemostasis"},{"key":"ref7","first-page":"1146","article-title":"Evaluation of an in vitro bleeding time device","volume":"69","author":"R Aishameeri","year":"1994","journal-title":"Thromb Haemost"},{"issue":"04","key":"ref8","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1007\/BF02471469","article-title":"Clinical application of a new in vitro bleeding time device on surgical patients","volume":"18","author":"T Tsujinaka","year":"1988","journal-title":"Jpn J Surg"}],"container-title":["Seminars in Thrombosis and Hemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0043-1777308.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T11:48:17Z","timestamp":1730807297000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0043-1777308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,12]]},"references-count":7,"journal-issue":{"issue":"02","published-online":{"date-parts":[[2024,2,5]]},"published-print":{"date-parts":[[2024,3]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0043-1777308","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0094-6176","1098-9064"],"issn-type":[{"value":"0094-6176","type":"print"},{"value":"1098-9064","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,12]]}}}