{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:48:04Z","timestamp":1792514884208,"version":"4.5.0"},"reference-count":17,"publisher":"Georg Thieme Verlag KG","issue":"03","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n               Objective\u2003This study aimed to evaluate the diagnostic sensitivity and specificity of the active matrix metalloproteinase-8 (aMMP-8) quantitative chairside point-of-care (PoC) lateral flow immunotest for peri-implant diseases, and it sought to correlate aMMP-8 levels with clinical parameters to determine its effectiveness as a biomarker for peri-implantitis.<\/jats:p><jats:p>\n               Materials and Methods\u2003A cross-sectional study was conducted at the Department of Periodontology and Implant Biology, Aristotle University of Thessaloniki, Greece. Participants included systemically healthy individuals with at least one implant loaded for more than 1 year, who had not received periodontal treatment or antibiotics in the preceding 6 months. Exclusion criteria included diabetes and immune-compromising conditions. Peri-implant sulcular fluid (PISF) samples were collected from the mesiobuccal or distobuccal site of the implant. The quantitative chairside PoC lateral flow immunotest for peri-implant diseases (ImplantSafe test) and ORALyzer digital reader were used to analyze PISF, with results expressed in ng\/mL. Clinical parameters such as bleeding on probing (BOP), probing depth (PD), recession (REC), and clinical attachment level (CAL) were measured at six sites per implant using a 15-mm scale periodontal probe.<\/jats:p><jats:p>\n               Results\u2003No significant differences were found in age, gender distribution, or smoking status between the healthy\/mucositis and peri-implantitis groups. The peri-implantitis group showed significantly higher mean percentages of BOP (57.58\u2009\u00b1\u200931.73 vs. 18.79\u2009\u00b1\u200924.17), PD (4.59\u2009\u00b1\u20091.22\u2009mm vs. 2.94\u2009\u00b1\u20090.78\u2009mm), and CAL (5.21\u2009\u00b1\u20091.72\u2009mm vs. 3.05\u2009\u00b1\u20090.81\u2009mm). aMMP-8 levels were also significantly higher in the peri-implantitis group (53.39\u2009\u00b1\u200949.70 vs. 22.03\u2009\u00b1\u200932.87). The diagnostic test demonstrated a sensitivity of 81.25% and specificity of 74.07%, with an area under the curve of 79.6%, indicating overall good accuracy in distinguishing between positive and negative conditions.<\/jats:p><jats:p>\n               Conclusion\u2003The aMMP-8 is a promising biomarker for peri-implantitis, showing elevated levels in affected patients. The aMMP-8 chairside test demonstrates high diagnostic sensitivity and specificity, supporting its use in early detection and monitoring of peri-implant diseases. Further research is needed to establish standardized protocols for its clinical application and to explore its long-term predictive value in implant care.<\/jats:p>","DOI":"10.1055\/s-0044-1793843","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:19:53Z","timestamp":1733876393000},"page":"743-748","source":"Crossref","is-referenced-by-count":10,"title":["Diagnostic Accuracy of Active MMP-8 Point-of-Care Test in Peri-Implantitis"],"prefix":"10.1055","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2156-0686","authenticated-orcid":false,"given":"Ioannis","family":"Fragkioudakis","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Implant Biology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonidas","family":"Batas","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Implant Biology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Vouros","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Implant Biology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitra","family":"Sakellari","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Implant Biology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2024,12,10]]},"reference":[{"issue":"04","key":"ref1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.jpor.2012.07.002","article-title":"Matrix metalloproteinase-8 is the major potential collagenase in active peri-implantitis","volume":"56","author":"H Arakawa","year":"2012","journal-title":"J Prosthodont Res"},{"key":"ref2","first-page":"352","article-title":"Matrix metalloproteinases in periodontal tissue remodelling","volume":"1","author":"J Sodek","year":"1992","journal-title":"Matrix Suppl"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"7891323","DOI":"10.1155\/2018\/7891323","article-title":"Matrix metalloproteinase-8 as an inflammatory and prevention biomarker in periodontal and peri-implant diseases","volume":"2018","author":"A Al-Majid","year":"2018","journal-title":"Int J Dent"},{"issue":"01","key":"ref4","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1601-0825.2009.01603.x","article-title":"Detection of gingival crevicular fluid MMP-8 levels with different laboratory and chair-side methods","volume":"16","author":"T Sorsa","year":"2010","journal-title":"Oral 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adolescents","volume":"9","author":"I T R\u00e4is\u00e4nen","year":"2019","journal-title":"Diagnostics (Basel)"},{"issue":"11","key":"ref8","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1111\/jcpe.13521","article-title":"On the accuracy, sensitivity, and grading of mouthrinse active matrix metalloproteinase-8 (aMMP-8) point-of-care testing (POCT)","volume":"48","author":"T Sorsa","year":"2021","journal-title":"J Clin Periodontol"},{"issue":"01","key":"ref9","doi-asserted-by":"crossref","first-page":"17069","DOI":"10.1038\/nrdp.2017.69","article-title":"A quantitative point-of-care test for periodontal and dental peri-implant diseases","volume":"3","author":"T Sorsa","year":"2017","journal-title":"Nat Rev Dis Primers"},{"issue":"03","key":"ref10","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1055\/s-0042-1753454","article-title":"Active MMP-8 as a biomarker of peri-implant health or disease","volume":"17","author":"V Xanthopoulou","year":"2023","journal-title":"Eur J Dent"},{"issue":"S 20","key":"ref11","first-page":"S9","article-title":"Periodontal health","volume":"45","author":"N P Lang","year":"2018","journal-title":"J Clin Periodontol"},{"issue":"S 20","key":"ref12","first-page":"S246","article-title":"Peri-implantitis","volume":"45","author":"F Schwarz","year":"2018","journal-title":"J Clin Periodontol"},{"issue":"02","key":"ref13","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1002\/cre2.537","article-title":"Active MMP-8 point-of-care (PoC)\/chairside enzyme-test as an adjunctive tool for early and real-time diagnosis of peri-implantitis","volume":"8","author":"H L\u00e4hteenm\u00e4ki","year":"2022","journal-title":"Clin Exp Dent Res"},{"issue":"04","key":"ref14","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1034\/j.1600-0765.2003.00677.x","article-title":"Gingival crevicular fluid collagenase-2 (MMP-8) test stick for chair-side monitoring of 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