{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T11:58:29Z","timestamp":1781179109469,"version":"3.54.1"},"reference-count":18,"publisher":"Scientific Scholar","issue":"03","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["South Asian J Cancer"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The diagnosis of cutaneous T-cell lymphoma (CTCL) is sometimes difficult. Detection of monoclonal T-cell receptor gamma (TCRG) gene rearrangement by polymerase chain reaction (PCR) has become an important adjunct to the diagnosis of CTCL. This study was designed to explore the concordance in terms of the diagnostic value of BIOMED-2 TCRG PCR protocol with the histological diagnosis.<\/jats:p>\n                  <jats:p>Confirmed and doubtful CTCLs were included in this descriptive cross-sectional study performed in the Habib Thameur Hospital in 2021. These cases were followed in the department of dermatology from 2012 to 2021. PCR tests were performed with TCRG BIOMED-2 clonality methods followed by capillary electrophoresis and GeneScan analysis. Clonality and statistical results were analyzed.<\/jats:p>\n                  <jats:p>Monoclonality was identified in 51% of confirmed CTCL cases (16\/28 cases with confirmed mycosis fungoides and 2\/7 other CTCL cases) and in 63% of doubtful cases, which were converted to malignant diagnosis. The results of TCRG clonality demonstrated a significant correlation with histopathology diagnoses of specimens. A moderate concordance was found between histology and molecular clonality.<\/jats:p>\n                  <jats:p>Results from this molecular clonality emphasize the importance of interpreting data in association with histopathological features of the lesions.<\/jats:p>","DOI":"10.1055\/s-0045-1805056","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T18:54:11Z","timestamp":1741373651000},"page":"627-631","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Utility of Molecular Analysis in Cutaneous T-Cell Lymphoma: Tunisian Series on Clonality of TCRG Gene Rearrangement"],"prefix":"10.25259","volume":"14","author":[{"given":"Sarra","family":"Klai","sequence":"additional","affiliation":[{"name":"Department of Pathology, Habib Thameur Hospital, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imen","family":"Helal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Habib Thameur Hospital, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raja","family":"Jouini","sequence":"additional","affiliation":[{"name":"Department of Pathology, Habib Thameur Hospital, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houda","family":"Hammami","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Habib Thameur Hospital, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maher","family":"Kharrat","sequence":"additional","affiliation":[{"name":"LR99ES10 Human Genetics Laboratory, Faculty of Medicine of Tunis (FMT), University of Tunis El Manar, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Fenniche","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Habib Thameur Hospital, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Khanchel","sequence":"additional","affiliation":[{"name":"Department of Pathology, Habib Thameur Hospital, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aschraf","family":"Chadli-Debbiche","sequence":"additional","affiliation":[{"name":"Department of Pathology, Habib Thameur Hospital, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2025,3,7]]},"reference":[{"issue":"01","key":"ref1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/s41572-021-00296-9","article-title":"Cutaneous T cell lymphoma","volume":"7","author":"R Dummer","year":"2021","journal-title":"Nat Rev Dis Primers"},{"issue":"02","key":"ref2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/j.1365-2141.2011.08852.x","article-title":"Evolving insights in the pathogenesis and therapy of cutaneous T-cell lymphoma (mycosis fungoides and Sezary syndrome)","volume":"155","author":"H K Wong","year":"2011","journal-title":"Br J Haematol"},{"issue":"308","key":"ref3","doi-asserted-by":"crossref","first-page":"308ra158","DOI":"10.1126\/scitranslmed.aaa9122","article-title":"TCR sequencing facilitates diagnosis and identifies mature T cells as the cell of origin in CTCL","volume":"7","author":"I R Kirsch","year":"2015","journal-title":"Sci Transl Med"},{"issue":"09","key":"ref4","first-page":"1307","article-title":"Mycosis fungoides and S\u00e9zary syndrome","volume":"19","author":"C Jonak","year":"2021","journal-title":"J Dtsch Dermatol Ges"},{"issue":"03","key":"ref5","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1136\/jclinpath-2017-204761","article-title":"T cell clonality assessment: past, present and future","volume":"71","author":"E Mahe","year":"2018","journal-title":"J Clin Pathol"},{"issue":"01","key":"ref6","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jmoldx.2011.07.007","article-title":"Application of microfluidic technology to the BIOMED-2 protocol for detection of B-cell clonality","volume":"14","author":"A Zam\u00f2","year":"2012","journal-title":"J Mol Diagn"},{"issue":"10","key":"ref7","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1038\/leu.2012.246","article-title":"EuroClonality\/BIOMED-2 guidelines for interpretation and reporting of Ig\/TCR clonality testing in suspected lymphoproliferations","volume":"26","author":"A W Langerak","year":"2012","journal-title":"Leukemia"},{"issue":"12","key":"ref8","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1038\/sj.leu.2403202","article-title":"Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936","volume":"17","author":"J JM van Dongen","year":"2003","journal-title":"Leukemia"},{"issue":"03","key":"ref9","doi-asserted-by":"crossref","first-page":"320","DOI":"10.2353\/jmoldx.2010.090123","article-title":"Combined use of PCR-based TCRG and TCRB clonality tests on paraffin-embedded skin tissue in the differential diagnosis of mycosis fungoides and inflammatory dermatoses","volume":"12","author":"B Zhang","year":"2010","journal-title":"J Mol Diagn"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.3389\/fonc.2019.01558","article-title":"An update on molecular biology of cutaneous T cell lymphoma","volume":"9","author":"R Walia","year":"2020","journal-title":"Front Oncol"},{"issue":"09","key":"ref11","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.3109\/10428194.2013.871634","article-title":"Detection of T-cell receptor \u03b3 gene rearrangement in paraffin-embedded T or natural killer\/T-cell lymphoma samples using the BIOMED-2 protocol","volume":"55","author":"T Miyata-Takata","year":"2014","journal-title":"Leuk Lymphoma"},{"issue":"04","key":"ref12","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1097\/01.pas.0000153121.57515.c6","article-title":"Histopathologic features of early (patch) lesions of mycosis fungoides: a morphologic study on 745 biopsy specimens from 427 patients","volume":"29","author":"C Massone","year":"2005","journal-title":"Am J Surg Pathol"},{"issue":"01","key":"ref13","doi-asserted-by":"crossref","first-page":"349","DOI":"10.3892\/or.2020.7865","article-title":"Utility of flow cytometry and gene rearrangement analysis in tissue and blood of patients with suspected cutaneous T\u2013cell lymphoma","volume":"45","author":"J D Gibbs","year":"2021","journal-title":"Oncol Rep"},{"issue":"04","key":"ref14","doi-asserted-by":"crossref","first-page":"264","DOI":"10.5732\/cjc.010.10344","article-title":"Diagnostic significance of TCR gene clonal rearrangement analysis in early mycosis fungoides","volume":"30","author":"C Xu","year":"2011","journal-title":"Chin J Cancer"},{"issue":"07","key":"ref15","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1097\/00000478-199707000-00001","article-title":"The value of the polymerase chain reaction in the diagnosis of cutaneous T-cell infiltrates","volume":"21","author":"M Ashton-Key","year":"1997","journal-title":"Am J Surg Pathol"},{"issue":"04","key":"ref16","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0929-6646(09)60251-5","article-title":"Histopathologic-molecular correlation in early mycosis fungoides using T-cell receptor gamma gene rearrangement by polymerase chain reaction with laser capture microdissection","volume":"106","author":"P-F Hsiao","year":"2007","journal-title":"J Formos Med Assoc"},{"issue":"12","key":"ref17","doi-asserted-by":"crossref","first-page":"153279","DOI":"10.1016\/j.prp.2020.153279","article-title":"T-cell receptor antibodies expression in benign and malignant cutaneous lymphoid infiltrates in comparison with T-cell receptor gene rearrangement and its diagnostic utility in borderline cases","volume":"216","author":"K Kamar\u00e1dov\u00e1","year":"2020","journal-title":"Pathol Res Pract"},{"issue":"04","key":"ref18","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/S1525-1578(10)60580-6","article-title":"BIOMED-2 multiplex immunoglobulin\/T-cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics","volume":"7","author":"Y Sandberg","year":"2005","journal-title":"J Mol Diagn"}],"container-title":["South Asian Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0045-1805056.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T11:13:15Z","timestamp":1781176395000},"score":1,"resource":{"primary":{"URL":"https:\/\/journal.sajc.org\/diagnostic-utility-of-molecular-analysis-in-cutaneous-t-cell-lymphoma-tunisian-series-on-clonality-of-tcrg-gene-rearrangement\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,7]]},"references-count":18,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2025,12,23]]},"published-print":{"date-parts":[[2025,7]]}},"URL":"https:\/\/doi.org\/10.1055\/s-0045-1805056","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["2278-330X","2278-4306"],"issn-type":[{"value":"2278-330X","type":"print"},{"value":"2278-4306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,7]]}}}