{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T17:25:04Z","timestamp":1785173104045,"version":"3.55.0"},"reference-count":52,"publisher":"Georg Thieme Verlag KG","issue":"04","funder":[{"name":"Italian Ministry of Health Ricerca corrente","award":["2023"],"award-info":[{"award-number":["2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Semin Thromb Hemost"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The thrombin generation assay (TGA), originally developed by McFarlane and Biggs in 1956, was modified in the 2000s by Hemker and coworkers. TGA aims to monitor the continuous generation of thrombin upon activation of coagulation in plasma by the addition of such triggers as small amounts of tissue factor, synthetic phospholipids, and calcium chloride. TGA is sensitive to hypo- and hypercoagulability and is affected by prohemostatic as well as antithrombotic drugs. The review of the current literature shows that TGA is mainly used to investigate conditions characterized by hypo- as well as hypercoagulability and as a laboratory tool to elucidate coagulation mechanisms that are not yet completely understood. This article aims to overview the value and limits of current procedures for TGA for the investigation of hemostasis.<\/jats:p>","DOI":"10.1055\/s-0045-1807261","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T18:51:46Z","timestamp":1744051906000},"page":"441-447","source":"Crossref","is-referenced-by-count":1,"title":["Thrombin Generation Assays: What are the Current Clinical Applications?"],"prefix":"10.1055","volume":"52","author":[{"given":"Armando","family":"Tripodi","sequence":"additional","affiliation":[{"name":"IRCCS Ca' Granda Maggiore Hospital Foundation and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marigrazia","family":"Clerici","sequence":"additional","affiliation":[{"name":"IRCCS Ca' Granda Maggiore Hospital Foundation and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Scalambrino","sequence":"additional","affiliation":[{"name":"IRCCS Ca' Granda Maggiore Hospital Foundation and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flora","family":"Peyvandi","sequence":"additional","affiliation":[{"name":"IRCCS Ca' Granda Maggiore Hospital Foundation and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Milano, Italy"},{"name":"Department of Pathophysiology and Transplantation, Universit\u00e0 degli Studi di Milano, Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"issue":"01","key":"ref1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1136\/jcp.6.1.3","article-title":"A thrombin generation test; the application in haemophilia and thrombocytopenia","volume":"6","author":"R G MacFarlane","year":"1953","journal-title":"J Clin Pathol"},{"issue":"01","key":"ref2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1055\/s-0038-1661594","article-title":"A computer assisted method to obtain the prothrombin activation velocity in whole plasma independent of thrombin decay processes","volume":"56","author":"H C Hemker","year":"1986","journal-title":"Thromb Haemost"},{"issue":"04","key":"ref3","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1055\/s-0038-1649638","article-title":"Continuous registration of thrombin generation in plasma, its use for the determination of the thrombin potential","volume":"70","author":"H C Hemker","year":"1993","journal-title":"Thromb Haemost"},{"issue":"04","key":"ref4","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1055\/s-0037-1613868","article-title":"The thrombogram: monitoring thrombin generation in platelet-rich plasma","volume":"83","author":"H C Hemker","year":"2000","journal-title":"Thromb Haemost"},{"issue":"05","key":"ref5","first-page":"547","article-title":"Thrombin generation assessed as endogenous thrombin potential in patients with hyper- or hypo-coagulability","volume":"88","author":"V Chantarangkul","year":"2003","journal-title":"Haematologica"},{"issue":"05","key":"ref6","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1111\/jth.15287","article-title":"Recommendations for the measurement of thrombin generation: Communication from the ISTH SSC Subcommittee on Lupus Anticoagulant\/Antiphospholipid Antibodies","volume":"19","author":"M Ninivaggi","year":"2021","journal-title":"J Thromb Haemost"},{"issue":"09","key":"ref7","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.3324\/haematol.2019.221655","article-title":"Thrombin generation in cardiovascular disease and mortality - results from the Gutenberg Health Study","volume":"105","author":"P CS van Paridon","year":"2020","journal-title":"Haematologica"},{"issue":"02","key":"ref8","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1002\/hep.21266","article-title":"Thrombin generation in patients with cirrhosis: the role of platelets","volume":"44","author":"A Tripodi","year":"2006","journal-title":"Hepatology"},{"issue":"05","key":"ref9","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1160\/TH16-10-0806","article-title":"Detection of procoagulant imbalance. 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