{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T23:10:06Z","timestamp":1749942606067,"version":"3.41.0"},"reference-count":30,"publisher":"Georg Thieme Verlag KG","issue":"02","license":[{"start":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T00:00:00Z","timestamp":1749859200000},"content-version":"vor","delay-in-days":74,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Bras Ortop (Sao Paulo)"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Resumo<\/jats:title><jats:p>\n               Objetivo\u2003O desafio de consolida\u00e7\u00e3o de fraturas osteopor\u00f3ticas, particularmente exacerbadas pela defici\u00eancia de estrog\u00eanio p\u00f3s-menopausa, ressalta a necessidade urgente de interven\u00e7\u00f5es eficazes. Este estudo tem como objetivo avaliar o impacto do estrog\u00eanio administrado localmente via nanoc\u00e1psulas na consolida\u00e7\u00e3o de fraturas osteopor\u00f3ticas em ratas ovariectomizadas e analisar os efeitos sist\u00eamicos deste horm\u00f4nio usando o \u00fatero como \u00f3rg\u00e3o sentinela.<\/jats:p><jats:p>\n               M\u00e9todos\u2003Quarenta e cinco animais foram submetidos a fraturas femorais padronizadas e divididos em tr\u00eas grupos: G1 (controle), G2 (estrog\u00eanio convencional) e G3 (nanoc\u00e1psulas de estrog\u00eanio). O estrog\u00eanio foi aplicado topicamente na regi\u00e3o da incis\u00e3o da pele (\u00e1rea tricotomizada). A cicatriza\u00e7\u00e3o da fratura foi avaliada 15 e 30 dias ap\u00f3s a fratura por meio de an\u00e1lises radiogr\u00e1ficas e histol\u00f3gicas. A histologia uterina analisou os efeitos sist\u00eamicos.<\/jats:p><jats:p>\n               Resultados\u2003Na an\u00e1lise radiogr\u00e1fica dos calos \u00f3sseos, G3 (8,75\u2009\u00b1\u20090,77\u2009mm) exibiu forma\u00e7\u00e3o de calo significativamente maior do que o grupo controle (7,18\u2009\u00b1\u20090,4\u2009mm) no dia 15 e a an\u00e1lise histol\u00f3gica revelou aumento da forma\u00e7\u00e3o de calo em G3 no dia 30, indicando um processo de cicatriza\u00e7\u00e3o acelerado. Al\u00e9m disso, a an\u00e1lise histol\u00f3gica uterina no dia 30 mostrou uma redu\u00e7\u00e3o na espessura endometrial em G3 (510.073\u2009\u00b1\u200954.705,11 \u03bcm) em compara\u00e7\u00e3o a G2 (623.729\u2009\u00b1\u2009101.592 \u03bcm).<\/jats:p><jats:p>\n               Conclus\u00e3o\u2003Estes achados sugerem que nanoc\u00e1psulas t\u00f3picas de estrog\u00eanio podem aumentar a forma\u00e7\u00e3o de calos no tratamento de fraturas femorais osteopor\u00f3ticas em ratas, potencialmente com menos efeitos sist\u00eamicos.<\/jats:p>","DOI":"10.1055\/s-0045-1809513","type":"journal-article","created":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T22:30:16Z","timestamp":1749940216000},"page":"001-007","source":"Crossref","is-referenced-by-count":0,"title":["A aplica\u00e7\u00e3o t\u00f3pica de nanoc\u00e1psulas de estrog\u00eanio na incis\u00e3o cut\u00e2nea melhora a Consolida\u00e7\u00e3o de fraturas em ratas osteopor\u00f3ticas"],"prefix":"10.1055","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3815-6900","authenticated-orcid":false,"given":"Dalton","family":"Berri","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0147-7273","authenticated-orcid":false,"given":"Elcio","family":"Machinski","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0443-639X","authenticated-orcid":false,"given":"Conrado Auer","family":"Trentini","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9934-4027","authenticated-orcid":false,"given":"Paulo Vitor","family":"Farago","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9690-4287","authenticated-orcid":false,"given":"Adriana Yuriko","family":"Koga","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5662-622X","authenticated-orcid":false,"given":"Leandro Cavalcante","family":"Lipinski","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, 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Res"},{"issue":"05","key":"ref4","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/S0736-0266(02)00266-8","article-title":"Biomechanical evaluation of healing in a non-critical defect in a large animal model of osteoporosis","volume":"21","author":"C A Lill","year":"2003","journal-title":"J Orthop Res"},{"issue":"20","key":"ref5","doi-asserted-by":"crossref","first-page":"e902","DOI":"10.5435\/JAAOS-D-18-00600","article-title":"Diagnosis and Treatment of Osteoporosis: What Orthopaedic Surgeons Need to Know","volume":"27","author":"E G Matzkin","year":"2019","journal-title":"J Am Acad Orthop Surg"},{"issue":"04","key":"ref6","first-page":"857","article-title":"Effects of increased bone formation on fracture healing in mice","volume":"70","author":"F T Beil","year":"2011","journal-title":"J Trauma"},{"issue":"05","key":"ref7","first-page":"1259","article-title":"Effects of estrogen on fracture healing in mice","volume":"69","author":"F T Beil","year":"2010","journal-title":"J Trauma"},{"issue":"06","key":"ref8","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1007\/s11914-021-00702-x","article-title":"Osteocytes and Estrogen Deficiency","volume":"19","author":"L M McNamara","year":"2021","journal-title":"Curr Osteoporos Rep"},{"issue":"S 06","key":"ref9","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1515\/jpem-2000-s619","article-title":"Estrogen, bone, growth and sex: a sea change in conventional wisdom","volume":"13","author":"M M Grumbach","year":"2000","journal-title":"J Pediatr Endocrinol Metab"},{"issue":"01","key":"ref10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1210\/clinem\/dgaa764","article-title":"The Menopause Transition: Signs, Symptoms, and Management Options","volume":"106","author":"N Santoro","year":"2021","journal-title":"J Clin Endocrinol 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remodela\u00e7\u00e3o e repara\u00e7\u00e3o \u00f3ssea","volume":"42","author":"S U Amadei","year":"2006","journal-title":"J Bras Patol Med Lab"},{"issue":"S 01","key":"ref15","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s43465-023-01071-6","article-title":"Postmenopausal Osteoporosis: Menopause Hormone Therapy and Selective Estrogen Receptor Modulators","volume":"57","author":"J Rani","year":"2023","journal-title":"Indian J Orthop"},{"issue":"06","key":"ref16","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1007\/s00404-022-06647-5","article-title":"Effects of transdermal versus oral hormone replacement therapy in postmenopause: a systematic review","volume":"307","author":"M\u0160 Gold\u0161tajn","year":"2023","journal-title":"Arch Gynecol Obstet"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e241025","DOI":"10.1590\/1519-6984.241025","article-title":"Challenges of nanotechnology in cosmetic permeation with caffeine","volume":"82","author":"E M Vogel","year":"2021","journal-title":"Braz J Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1016631","DOI":"10.3389\/fphar.2022.1016631","article-title":"Editorial: Bone targeting nanoparticle drug delivery system in bone metabolism and bone-related tumor diseases","volume":"13","author":"X Wang","year":"2022","journal-title":"Front Pharmacol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40824-019-0166-x","article-title":"Therapeutic efficacy of nanoparticles and routes of administration","volume":"23","author":"D Chenthamara","year":"2019","journal-title":"Biomater Res"},{"key":"ref20","first-page":"1","volume-title":"A brief History of Nanotechnology and Introduction to Nanoparticles for biomedical Applications","author":"L Leon","year":"2020"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/s11051-024-06026-y","article-title":"Nanodispersions for drug delivery applications: a special focus toward cancer therapeutics","volume":"26","author":"V Sethuraman","year":"2024","journal-title":"J Nanopart Res"},{"issue":"03","key":"ref22","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1002\/jor.1100090310","article-title":"The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats","volume":"9","author":"M H Huo","year":"1991","journal-title":"J Orthop Res"},{"issue":"03","key":"ref23","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.3390\/ijms23031376","article-title":"Osteoporosis Due to Hormone Imbalance: An Overview of the Effects of Estrogen Deficiency and Glucocorticoid Overuse on Bone Turnover","volume":"23","author":"C H Cheng","year":"2022","journal-title":"Int J Mol Sci"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1222129","DOI":"10.3389\/fimmu.2023.1222129","article-title":"The effect of cytokines on osteoblasts and osteoclasts in bone remodeling in osteoporosis: a review","volume":"14","author":"J Xu","year":"2023","journal-title":"Front Immunol"},{"issue":"03","key":"ref25","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1007\/s00223-004-0197-7","article-title":"Action of estradiol on epiphyseal growth plate chondrocytes","volume":"75","author":"C Rodd","year":"2004","journal-title":"Calcif Tissue Int"},{"issue":"09","key":"ref26","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1002\/1529-0131(200009)43:9<2081::AID-ANR20>3.0.CO;2-I","article-title":"Functional estrogen receptors in adult articular cartilage: estrogen replacement therapy increases chondrocyte synthesis of proteoglycans and insulin-like growth factor binding protein 2","volume":"43","author":"R S Richmond","year":"2000","journal-title":"Arthritis Rheum"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.3389\/fnano.2020.584489","article-title":"Nanomaterials Versus The Microbial Compounds With Wound Healing 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