{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:20:54Z","timestamp":1780449654213,"version":"3.54.1"},"reference-count":44,"publisher":"Georg Thieme Verlag KG","issue":"02","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study evaluated the expression of active-matrix metalloproteinase-8 (aMMP-8) in saliva, oral rinse, and gingival crevicular fluid (GCF) of periodontitis patients with and without metabolic syndrome (MetS) and assessed correlations between aMMP-8 levels and both periodontal and metabolic parameters.<\/jats:p>\n                  <jats:p>One hundred and twenty participants aged 25 to 55 years were classified into three groups: MetS with periodontitis (MetS-PD, n\u2009=\u200940); systemically healthy with periodontitis (SH-PD, n\u2009=\u200940); and systemically and periodontally healthy controls (SH-PH, n\u2009=\u200940).<\/jats:p>\n                  <jats:p>The median age of the participants was 49.5\u2009\u00b1\u20098.94 in MetS-PD, 47.5\u2009\u00b1\u20095.6 in SH-PD, and 42.5\u2009\u00b1\u200911.14 in SH-PH. Systemic parameters (waist circumference, blood pressure, lipid profile, and glycemic indices) and periodontal parameters (bleeding on probing, plaque score, probing depth, and clinical attachment loss) were assessed. aMMP-8 was evaluated qualitatively using point-of-care testing (PoCT) and quantitatively via ELISA in oral rinse, saliva, and GCF.<\/jats:p>\n                  <jats:p>The PoCT was positive in 90% of MetS-PD and 85% of SH-PD participants, while all SH-PH participants tested negative (p\u2009&lt;\u20090.01). MetS-PD exhibited significantly higher aMMP-8 levels in oral rinse and saliva than SH-PD (p\u2009=\u20090.001), whereas GCF aMMP-8 levels were higher in SH-PD but not substantially different from MetS-PD (p\u2009=\u20090.086). Strong positive correlations were observed between aMMP-8 levels in all oral matrices and periodontal parameters (p\u2009&lt;\u20090.001). Among metabolic parameters, waist circumference, triglycerides, low-density lipoprotein, and fasting blood sugar showed stronger correlations with aMMP-8 levels than blood pressure measurements.<\/jats:p>\n                  <jats:p>The aMMP-8 PoCT effectively distinguished periodontally diseased individuals from healthy controls. Elevated aMMP-8 levels (&gt;20\u2009ng\/mL) in oral fluids, especially saliva and oral rinse, were significantly associated with both periodontal and systemic risk factors, supporting the potential of aMMP-8 as a noninvasive biomarker for periodontitis and metabolic disorders.<\/jats:p>","DOI":"10.1055\/s-0045-1809977","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:25:33Z","timestamp":1753316733000},"page":"407-419","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Potential of aMMP-8 PoCT in Oral Fluids: Insights from Periodontitis and Metabolic Syndrome Interplay"],"prefix":"10.1055","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6390-2621","authenticated-orcid":false,"given":"Julie Toby","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland"},{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betsy","family":"Joseph","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland"},{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland"},{"name":"Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukumaran","family":"Anil","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok, Thailand"},{"name":"Global Research Cell, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuomas","family":"Waltimo","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland"},{"name":"Faculty of Medicine, University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,7,23]]},"reference":[{"issue":"06","key":"ref1","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1111\/jcpe.13807","article-title":"Association between periodontal diseases and cardiovascular diseases, diabetes and respiratory diseases: consensus report of the Joint Workshop by the European Federation of Periodontology (EFP) and the European arm of the World Organization of Family Doctors (WONCA Europe)","volume":"50","author":"D Herrera","year":"2023","journal-title":"J Clin 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