{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T23:15:08Z","timestamp":1783379708525,"version":"3.54.6"},"reference-count":35,"publisher":"Georg Thieme Verlag KG","issue":"03","license":[{"start":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:00:00Z","timestamp":1773446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tongue cancer represents nearly half of all oral cancer cases globally, necessitating the exploration of novel therapeutic agents. Catharanthus roseus, known for its well-established anticancer properties, offers a promising alternative. Although C. roseus has been studied in several cancer models, its effects in tongue cancer cells, particularly in relation to phosphatidylinositol-3-kinase (PI3K) and poly(adenosine diphosphate-ribose) polymerase (PARP) pathways, have not been evaluated. This study examined the effect of the ethanol extract of C. roseus leaf (EECRL) on HSC-3 cells.<\/jats:p>\n                  <jats:p>Cells were treated with various concentrations of EECRL. Cell viability was assessed using the MTT assay, apoptosis was evaluated by sub-G1 analysis, and levels of cleaved-PARP and phosphorylated PI3K were measured using enzyme-linked immunosorbent assay.<\/jats:p>\n                  <jats:p>Data normality was assessed using the Shapiro\u2013Wilk test. Depending on data distribution, comparisons were performed using one-way analysis of variance followed by Tukey's post hoc test or the Kruskal\u2013Wallis test followed by Dunn's post hoc test. Correction for multiple comparisons was applied using the Holm\u2013Bonferroni method, and adjusted p-values were used to determine statistical significance.<\/jats:p>\n                  <jats:p>EECRL reduced number of viable cells and induced apoptosis in HSC-3 cells in a significant, concentration-dependent manner (adjusted p-value\u2009&lt;\u20090.05). Additionally, EECRL significantly decreased the activity of PI3K in a concentration-dependent manner (adjusted p-value\u2009&lt;\u20090.05) and significantly increased cleaved-PARP compared with the untreated group (adjusted p-value\u2009&lt;\u20090.05).<\/jats:p>\n                  <jats:p>EECRL reduced viable cell numbers and induced apoptotic features in HSC-3 tongue cancer cells, with effects associated with suppression of PI3K signaling and increased PARP cleavage. These findings suggest that EECRL may have potential as an alternative therapeutic candidate for tongue cancer. However, further mechanistic studies are required to confirm causal pathway involvement.<\/jats:p>","DOI":"10.1055\/s-0046-1816555","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T23:32:46Z","timestamp":1773531166000},"page":"867-876","source":"Crossref","is-referenced-by-count":0,"title":["Ethanol Extract of Catharanthus roseus Leaves Exhibits Anticancer Effects on HSC-3 Tongue Cancer Cells Associated with PI3K Suppression and PARP Cleavage"],"prefix":"10.1055","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5852-1074","authenticated-orcid":false,"given":"Ferry","family":"Sandra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Indonesia"},{"name":"Center of Molecular Biology Study, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie Sadono","family":"Djamil","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2370-038X","authenticated-orcid":false,"given":"Muhammad Ihsan","family":"Rizal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Indonesia"},{"name":"Center of Molecular Biology Study, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ria Aryani","family":"Hayuningtyas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Indonesia"},{"name":"Center of Molecular Biology Study, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackson","family":"Dipankara","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nur'amalia","family":"Isnaeni","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Ryan","family":"Krisdianto","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerinillia","family":"Mochtar","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Hoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Institute, Ballys Co. 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