{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T23:45:24Z","timestamp":1775864724368,"version":"3.50.1"},"reference-count":37,"publisher":"Georg Thieme Verlag KG","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To evaluate the potential of a phosphoprotein analogue to mediate mineral deposition and enhance surface integrity in deep carious dentin lesions, compared with mineral trioxide aggregate (MTA) and Sylc air abrasion.<\/jats:p>\n                  <jats:p>Thirty carious dentin discs (3.5\u2009\u00b1\u20090.1\u2009mm in thickness) were prepared and examined visually (ranging from yellow to light brown), through tactile assessment (leathery sensation), with 0\u201320 DIAGNOdent pen readings indicating caries-affected dentin (CAD) substrate. The specimens received three treatments: dentin matrix protein-1 (DMP-1, MCE, United States), MTA (Angelus, Brazil), and Sylc air abrasion (AquaCare, United Kingdom), and were immersed in artificial saliva (pH 7, 37\u00b0C) for 30 days. Fourier-transform infrared spectroscopy (FTIR) and contact angle measurement were performed on DMP-1. The mineral and organic content was assessed using Raman micro-spectroscopy, while cross-sectional tissue hardness was evaluated by a Vickers tester at 50 and 100\u2009\u00b5m depth from the treated surfaces. The morphological assessment was conducted using scanning electron microscopy (SEM).<\/jats:p>\n                  <jats:p>One-way ANOVA followed by Tukey's multiple comparison test and paired t-test were performed for the statistical analyses.<\/jats:p>\n                  <jats:p>The FTIR spectra of DMP-1 displayed the amide bands associated with carbonyl and N\u2013H stretching vibrations, in addition to the phosphate band. DMP-1 exhibited good wettability (contact angle of 27\u00b0), and enhanced the phosphate and organic content and tissue hardness of the treated CAD surfaces compared with pretreatment values (p\u2009&lt;\u20090.05). Meanwhile, the MTA-treated surface demonstrated the highest phosphate content and Vickers hardness number up to 100\u2009\u00b5m (p\u2009=\u20090.005). The SEM showed compact surfaces completely covered with mineral precipitation.<\/jats:p>\n                  <jats:p>DMP-1 provided a successful biomimetic analogue that enhanced the mineral\u2013organic phases of mineral-depleted dentin surfaces, offering an effective treatment modality for deep carious lesions compared with MTA and Sylc air abrasion.<\/jats:p>","DOI":"10.1055\/s-0046-1817164","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T22:56:08Z","timestamp":1775861768000},"source":"Crossref","is-referenced-by-count":0,"title":["A Biomimetic Approach for Treating Deep Dentin Carious Lesions Using Dentin Matrix Protein (DMP-1): An In Vitro Comparative Study"],"prefix":"10.1055","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1305-1950","authenticated-orcid":false,"given":"Ahmed F.","family":"Al-Thabhawee","sequence":"additional","affiliation":[{"name":"Department of Conservative and Aesthetic Dentistry, Baghdad College of Dentistry, University of Baghdad, Baghdad, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2068-4959","authenticated-orcid":false,"given":"Lamis A.","family":"Al-Taee","sequence":"additional","affiliation":[{"name":"Department of Conservative and Aesthetic Dentistry, Baghdad College of Dentistry, University of Baghdad, Baghdad, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3205-345X","authenticated-orcid":false,"given":"Hamdi H.","family":"Hamama","sequence":"additional","affiliation":[{"name":"Conservative Dentistry Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt"},{"name":"Conservative Dentistry Department, Faculty of Oral and Dental Medicine, Alsalam University, Tanta, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8363-9461","authenticated-orcid":false,"given":"Danya","family":"Hashem","sequence":"additional","affiliation":[{"name":"Department of Restorative Dental Science, College of Dentistry, Taibah University, Madinah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"issue":"01","key":"ref1","doi-asserted-by":"crossref","first-page":"2123624","DOI":"10.1080\/20002297.2022.2123624","article-title":"Functional signatures of ex-vivo dental caries onset","volume":"14","author":"D G Moussa","year":"2022","journal-title":"J Oral Microbiol"},{"issue":"02","key":"ref2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1055\/s-0044-1795117","article-title":"A systematic review and meta-analysis of the prevalence of dental caries in the permanent teeth of Arab children","volume":"19","author":"H Abutayyem","year":"2025","journal-title":"Eur J Dent"},{"issue":"01","key":"ref3","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1055\/s-0040-1703419","article-title":"Citation classics on dental caries: a systematic review","volume":"14","author":"A I Arshad","year":"2020","journal-title":"Eur J Dent"},{"issue":"02","key":"ref4","doi-asserted-by":"crossref","first-page":"434","DOI":"10.3390\/jcm9020434","article-title":"Pulp-dentin tissue healing response: a discussion of current biomedical approaches","volume":"9","author":"D Shah","year":"2020","journal-title":"J Clin Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"e20240271","DOI":"10.1590\/1678-7757-2024-0271","article-title":"Biomimetic restorative dentistry: an evidence-based discussion of common myths","volume":"32","author":"A Reis","year":"2024","journal-title":"J Appl Oral 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Mater"},{"issue":"02","key":"ref9","doi-asserted-by":"crossref","first-page":"e3319","DOI":"10.1002\/btpr.3319","article-title":"Dentin regeneration based on tooth tissue engineering: a review","volume":"39","author":"E Azaryan","year":"2023","journal-title":"Biotechnol Prog"},{"issue":"13","key":"ref10","doi-asserted-by":"crossref","first-page":"211616","DOI":"10.3390\/ma12132116","article-title":"Remineralization of artificial dentin caries using dentin and enamel matrix proteins","volume":"12","author":"K B\u00e4chli","year":"2019","journal-title":"Materials (Basel)"},{"issue":"04","key":"ref11","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1055\/s-0040-1713073","article-title":"Mineral trioxide aggregate applications in endodontics: a review","volume":"14","author":"G Cervino","year":"2020","journal-title":"Eur J Dent"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1055\/s-0045-1809311","article-title":"Comparative analysis of the physical properties of three 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Al-Shareefi","year":"2022","journal-title":"Diagnostics (Basel)"},{"issue":"01","key":"ref22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.26477\/jbcd.v37i1.3910","article-title":"The effect of carbon quantum dots on the microhardness and the morphological characteristics of human dental enamel: in vitro study","volume":"37","author":"I S Alateya","year":"2025","journal-title":"J Baghdad Coll Dent"},{"issue":"01","key":"ref23","doi-asserted-by":"crossref","first-page":"e41","DOI":"10.1111\/cdoe.12025","article-title":"International Caries Detection and Assessment System (ICDAS) and its International Caries Classification and Management System (ICCMS) - methods for staging of the caries process and enabling dentists to manage caries","volume":"41","author":"N B Pitts","year":"2013","journal-title":"Community Dent Oral Epidemiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"104050","DOI":"10.1016\/j.jdent.2022.104050","article-title":"In-vitro adhesive and interfacial analysis of a phosphorylated resin polyalkenoate cement bonded to dental hard tissues","volume":"118","author":"L Al-Taee","year":"2022","journal-title":"J Dent"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"104714","DOI":"10.1016\/j.jdent.2023.104714","article-title":"The in-vitro development of novel enzyme-based chemo-mechanical caries removal agents","volume":"138","author":"H Al-Badri","year":"2023","journal-title":"J Dent"},{"issue":"02","key":"ref26","doi-asserted-by":"crossref","first-page":"211","DOI":"10.4103\/2278-344X.130603","article-title":"X-ray diffraction analysis of MTA-Plus, MTA-Angelus and DiaRoot BioAggregate","volume":"8","author":"Y Guven","year":"2014","journal-title":"Eur J Dent"},{"issue":"09","key":"ref27","doi-asserted-by":"crossref","first-page":"408","DOI":"10.3390\/dj13090408","article-title":"Biomechanical and morphological analyses of enamel white spot lesions treated by different therapeutic approaches (in vitro comparative study)","volume":"13","author":"L AH Al-Taee","year":"2025","journal-title":"Dent J"},{"issue":"01","key":"ref28","first-page":"4321925","article-title":"Effect of enamel caries lesion baseline severity on fluoride dose-response","volume":"2017","author":"F Lippert","year":"2017","journal-title":"Int J Dent"},{"issue":"04","key":"ref29","doi-asserted-by":"crossref","first-page":"E188","DOI":"10.2341\/21-048-L","article-title":"Interfacial bond strength and morphology of sound and caries-affected dentin surfaces bonded to two resin-modified glass ionomer cements","volume":"47","author":"R M Al-Hasan","year":"2022","journal-title":"Oper Dent"},{"issue":"04","key":"ref30","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.3758\/BRM.41.4.1149","article-title":"Statistical power analyses using G*Power 3.1: tests for correlation and regression analyses","volume":"41","author":"F Faul","year":"2009","journal-title":"Behav Res 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