{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:50:40Z","timestamp":1774486240645,"version":"3.50.1"},"reference-count":30,"publisher":"Georg Thieme Verlag KG","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aimed to evaluate the feasibility of incorporating injectable platelet-rich fibrin (i-PRF) into electrospun chitosan\u2013polyvinyl alcohol (PVA) nanofibers by assessing (1) swelling behavior after immersion in i-PRF, (2) potential interactions between components based on Fourier transform infrared spectroscopy (FTIR) profiles, and (3) initial in vitro cytocompatibility using human primary fibroblasts.<\/jats:p>\n                  <jats:p>Chitosan\u2013PVA nanofibers were fabricated by electrospinning and trimmed into 5-mm discs. Specimens were immersed in 0.5\u2009mL of i-PRF for six time points (5, 10, 15, 20, 25, and 30\u2009minutes), and swelling capacity was evaluated to determine the immersion time associated with the highest absorption. FTIR was performed to compare functional group profiles before and after i-PRF immersion. For cytocompatibility testing, 5-mm diameter nanofiber discs were soaked in 0.5-mL i-PRF for 10\u2009minutes and placed into wells seeded with human primary fibroblasts (5\u2009\u00d7\u2009103 cells\/well). After 24, 48, and 72-hour incubation at 37\u00b0C in a CO2 incubator, methylthiazol tetrazolium (MTT) reagent was added and incubated for an additional 4\u2009hours. Absorbance was measured using a multimode microplate reader, and cell viability was calculated as the percentage relative to the control group.<\/jats:p>\n                  <jats:p>The highest swelling was observed after 10\u2009minutes of immersion (p\u2009&lt;\u20090.05). FTIR spectra showed no additional peaks indicative of new chemical functional groups after immersion, suggesting that i-PRF incorporation occurred predominantly through physical interaction rather than formation of new covalent bonds. In the MTT assay, fibroblast viability in the i-PRF-immersed nanofiber group was comparable to the control (p\u2009&lt;\u20090.05), indicating no detectable cytotoxic effect under the tested conditions.<\/jats:p>\n                  <jats:p>Chitosan\u2013PVA nanofibers demonstrated an ability to absorb i-PRF, exhibiting comparable FTIR functional groups following immersion. This finding indicates that the predominant interaction mechanism is of a physical nature. A 72-hour MTT assay indicated that there was acceptable initial cytocompatibility with human primary fibroblasts. The study concluded that the chitosan\u2013PVA\/i-PRF construct appears to be a promising preliminary scaffold, but further work is required on morphology, mechanics, degradation, release kinetics, wider cell responses, and in vivo performance before claims can be made about periodontal regeneration or clinical efficacy.<\/jats:p>","DOI":"10.1055\/s-0046-1818557","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:09Z","timestamp":1774483209000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Chitosan\u2013Polyvinyl Alcohol Nanofiber Loaded with Injectable Platelet-Rich Fibrin as Promising Candidate for Periodontal Membrane"],"prefix":"10.1055","author":[{"given":"Vincensia Maria","family":"Karina","sequence":"additional","affiliation":[{"name":"Doctoral Study Program, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia"},{"name":"Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nur Rahman Ahmad Seno","family":"Aji","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osa Amila","family":"Hafiyyah","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mentari Salma","family":"Nurbaiti","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deniar Faizya","family":"Widhawati","sequence":"additional","affiliation":[{"name":"Periodontics Specialization Study Program, Faculty of Dentistry, Department of Periodontics, Universitas Gadjah Mada Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tasya","family":"Amira","sequence":"additional","affiliation":[{"name":"Periodontics Specialization Study Program, Faculty of Dentistry, Department of Periodontics, Universitas Gadjah Mada Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronny","family":"Martien","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Kusumaatmaja","sequence":"additional","affiliation":[{"name":"Department of Physics, Faculty Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwartarini","family":"Murdiastuti","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"issue":"01","key":"ref1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/jcp.25067","article-title":"Potential for stem cell-based periodontal therapy","volume":"231","author":"S H Bassir","year":"2016","journal-title":"J Cell Physiol"},{"key":"ref2","volume-title":"Platelet Rich Fibrin in Regenerative Dentistry: Biological Background and Clinical Indications","author":"M Fujioka-Kobayashi","year":"2017","edition":"1st ed."},{"issue":"03","key":"ref3","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1007\/s00784-018-2555-2","article-title":"Injectable platelet rich fibrin: cell content, morphological, and protein characterization","volume":"23","author":"H A Varela","year":"2019","journal-title":"Clin Oral Investig"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1515\/phys-2017-0125","article-title":"Fabrication and application of coaxial of polyvinyl alcohol\/chitosan nanofiber membranes","volume":"15","author":"T Kuo","year":"2017","journal-title":"Open Phys"},{"issue":"08","key":"ref5","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1007\/s00784-017-2063-9","article-title":"Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?","volume":"21","author":"R J Miron","year":"2017","journal-title":"Clin Oral Investig"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.msec.2014.03.065","article-title":"Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation","volume":"40","author":"L Diaz-Gomez","year":"2014","journal-title":"Mater Sci Eng C"},{"issue":"09","key":"ref7","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.3390\/polym11091376","article-title":"Analysis of drug release behavior utilizing the swelling characteristics of cellulosic nanofibers","volume":"11","author":"S W Ko","year":"2019","journal-title":"Polymers (Basel)"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.materresbull.2003.10.013","article-title":"Chemical synthesis of hydroxyapatite\/poly(\u03b5-caprolactone) composites","volume":"39","author":"D Choi","year":"2004","journal-title":"Mater Res Bull"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.eurpolymj.2017.04.012","article-title":"Inherent antibacterial activity and in vitro biocompatibility of hydrophilic polymer film containing chemically anchored sulfadiazine moieties","volume":"91","author":"A Morro","year":"2017","journal-title":"Eur Polym 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A"},{"issue":"06","key":"ref13","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1007\/s100590000030","article-title":"Expression and characterization of fibroblast growth factor 8 from Mexican axolotl, Ambystoma mexicanum","volume":"10","author":"S Y Lee","year":"2000","journal-title":"Mol Cells"},{"key":"ref14","volume-title":"Cell viability assays [introduction]","author":"T L Riss","year":"2016"},{"key":"ref15","first-page":"289","volume-title":"Nanobiomaterials in Clinical Dentistry","author":"K Subramani","year":"2013"},{"issue":"09","key":"ref16","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/j.dental.2008.01.010","article-title":"Cytotoxicity of silica-glass fiber reinforced composites","volume":"24","author":"G Meri\u00e7","year":"2008","journal-title":"Dent Mater"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"012004","DOI":"10.1088\/1757-899X\/367\/1\/012004","article-title":"Stabilization of PVA\/Chitosan\/TiO 2 nanofiber membrane with heat treatment and glutaraldehyde crosslink","volume":"367","author":"N Nasikhudin","year":"2018","journal-title":"IOP Conf Series Mater Sci Eng"},{"issue":"06","key":"ref18","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1111\/jocd.12955","article-title":"Fluid platelet-rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet-rich plasma","volume":"18","author":"X Wang","year":"2019","journal-title":"J Cosmet Dermatol"},{"issue":"28","key":"ref19","doi-asserted-by":"crossref","first-page":"9711","DOI":"10.1021\/acs.analchem.1c00867","article-title":"Functional group identification for FTIR spectra using image-based machine learning models","volume":"93","author":"A A Enders","year":"2021","journal-title":"Anal Chem"},{"issue":"01","key":"ref20","doi-asserted-by":"crossref","first-page":"14","DOI":"10.35970\/infotekmesin.v11i1.63","article-title":"Suwarno. Analisa Spektroskopi Inframerah Transformasi Fourier dan Gas Terlarut terhadap Perubahan Gugus Fungsi Komposisi Minyak Ester","volume":"11","author":"S Rahmat","year":"2020","journal-title":"Jurnal Infotekmesin"},{"issue":"46","key":"ref21","first-page":"12","article-title":"Comparison of transmission FT-IR, ATR, and DRIFT spectra: implication for assessment of bone bioapetite diagenesis","volume":"2014","author":"M M Beasley","year":"2014","journal-title":"J Archaeol Sci"},{"issue":"05","key":"ref22","doi-asserted-by":"crossref","first-page":"8778","DOI":"10.1021\/acsomega.9b00204","article-title":"Fabrication of poly(vinyl alcohol)\/chitosan\/Bidens pilosa composite electrospun nanofibers with enhanced antibacterial activities","volume":"4","author":"J Kegere","year":"2019","journal-title":"ACS Omega"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"118351","DOI":"10.1016\/j.carbpol.2021.118351","article-title":"Platelet-rich fibrin-loaded PCL\/chitosan core-shell fibers scaffold for enhanced osteogenic differentiation of mesenchymal stem cells","volume":"269","author":"A Rastegar","year":"2021","journal-title":"Carbohydr Polym"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/0016-2361(82)90014-X","article-title":"FTIR analysis of coal. 1. techniques and determination of hydroxyl concentrations","volume":"61","author":"P R Solomon","year":"1982","journal-title":"Fuel"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.enconman.2017.05.026","article-title":"Study on the co-pyrolysis of rice straw and high density polyethylene blends using TG-FTIR-MS","volume":"146","author":"X Kai","year":"2017","journal-title":"Energy Conservation and Management"},{"issue":"01","key":"ref26","doi-asserted-by":"crossref","first-page":"29","DOI":"10.31266\/at.v26i1.1439","article-title":"Pembuatan Serat Nano Menggunakan Metode Elektrospinning","volume":"26","author":"T Wahyudi","year":"2011","journal-title":"Arena Tekstil"},{"issue":"114","key":"ref27","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/j.ijbiomac.2018.03.115","article-title":"Surface modification of electrospun PVA\/chitosan nanofibers by dielectric barrier discharge plasma at atmospheric pressure and studies of their mechanical properties and biocompatibility","volume":"114","author":"P Das","year":"2018","journal-title":"Int J Biol Macromol"},{"key":"ref29","first-page":"3","article-title":"Comprehensive review summarizing effect electrospinning parameters and potential application of nanofibers in biomedical and biotechnology","author":"A Haider","year":"2015","journal-title":"Arab J Chem"},{"issue":"04","key":"ref30","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1590\/1980-5373-mr-2016-0618","article-title":"Effect of chitosan on the properties of electrospun fibers from mixed poly(vinyl alcohol)\/chitosan solutions","volume":"20","author":"R Goncalves","year":"2017","journal-title":"Mater Res"},{"issue":"01","key":"ref31","first-page":"31","article-title":"Dompeipen, Isolasi dan identifikasi kitin dan kitosan dari kulit udang Windu (Penaeus monodon) dengan spektroskopi inframerah","volume":"13","author":"J Edward","year":"2017","journal-title":"Majalah Biam"}],"container-title":["European Journal of Dentistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/s-0046-1818557.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:19Z","timestamp":1774483219000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/s-0046-1818557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,25]]},"references-count":30,"URL":"https:\/\/doi.org\/10.1055\/s-0046-1818557","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["1305-7456","1305-7464"],"issn-type":[{"value":"1305-7456","type":"print"},{"value":"1305-7464","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,25]]}}}