{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T22:52:01Z","timestamp":1777071121998,"version":"3.51.4"},"reference-count":41,"publisher":"Georg Thieme Verlag KG","issue":"02","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":23,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Brazil"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Otorhinolaryngol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Oxidative stress plays an important role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). The 8-Hydroxydeoxyguanosine (8-OHdG) is a major oxidative DNA adduct, and its levels have been noted as a triggering factor for inflammation-related carcinogenesis, but have not been assessed in CRSwNP. The MAPK\/ERK pathway importance in the pathogenesis of CRSwNP has previously been reported. Mutations in RAS family genes occur in neoplastic and non-neoplastic proliferative lesions, and it has been previously demonstrated that KRAS mutations do not occur in CRSwNP.<\/jats:p>\n                  <jats:p>To assess the levels of 8-OHdG in nasal polyps occurring in CRSwNP, to associate these levels with clinicopathological parameters, and to investigate HRAS and NRAS hotspot mutations in the same samples.<\/jats:p>\n                  <jats:p>Both the HRAS (codons 49 and 61) and NRAS (codon 61) mutations were investigated through Sanger sequencing, and the levels of 8-OHdG were determined using ELISA in 14 freshly collected snap-frozen samples of nasal polyps occurring in CRSwNP. The associations between the levels of 8-OHdG and patients' age, presence of asthma, and eosinophil counts in nasal polyps were tested.<\/jats:p>\n                  <jats:p>No mutations were detected. The association between higher levels of 8-OHdG and higher eosinophil counts was observed, whereas there was no association between it and age or the presence of asthma.<\/jats:p>\n                  <jats:p>Based on the results in the present cohort, higher levels of 8-OHdG, indicative of oxidative stress, are associated with higher eosinophil counts in CRSwNP. Additionally, HRAS and NRAS mutations do not occur in nasal polyps or occur at a very low frequency.<\/jats:p>","DOI":"10.1055\/s-0046-1819639","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T22:32:17Z","timestamp":1777069937000},"page":"001-006","source":"Crossref","is-referenced-by-count":0,"title":["Association between 8-OHdG Levels and Eosinophil Counts in Chronic Rhinosinusitis with Nasal Polyps"],"prefix":"10.1055","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4735-037X","authenticated-orcid":false,"given":"Tamara Silva","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-0336","authenticated-orcid":false,"given":"Let\u00edcia Martins","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3005-1736","authenticated-orcid":false,"given":"Elisa Carvalho","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6065-1484","authenticated-orcid":false,"given":"Rafaela Magalh\u00e3es Braga","family":"Amaral","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9146-2320","authenticated-orcid":false,"given":"Wilma Terezinha","family":"Anselmo-Lima","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3153-6292","authenticated-orcid":false,"given":"Edwin","family":"Tamashiro","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4212-1172","authenticated-orcid":false,"given":"Marina Gon\u00e7alves","family":"Diniz","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6605-5317","authenticated-orcid":false,"given":"Fabiana Cardoso Pereira","family":"Valera","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8770-8009","authenticated-orcid":false,"given":"Ricardo Santiago","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1580-4995","authenticated-orcid":false,"given":"Carolina Cavalieri","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"issue":"09","key":"ref1","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1111\/j.1398-9995.2011.02646.x","article-title":"Chronic rhinosinusitis in Europe\u2013an underestimated disease. 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