{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T23:38:47Z","timestamp":1777073927915,"version":"3.51.4"},"reference-count":40,"publisher":"Georg Thieme Verlag KG","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study evaluated the influence of incorporating graphene oxide (GO) nanoparticles (0.1, 0.25, and 0.5\u2009wt%) into resin-based pit and fissure sealants on their mechanical, physical, antibacterial, and cytocompatible properties, compared with a commercial reference material.<\/jats:p>\n                  <jats:p>Experimental sealants containing GO (G2\u2013G4) were prepared, with an unmodified resin as control (G1) and Helioseal F Plus as commercial comparison (G5). GO morphology was characterized by TEM. Specimens (n\u2009=\u2009240) were tested for Vickers microhardness, viscosity, surface roughness change (\u0394Ra) after simulated toothbrushing, and fluorescence (% blue channel). Antibacterial activity against Streptococcus mutans was assessed using a direct contact test (ASTM E2180), and antibacterial rate (AR) was calculated. Cytotoxicity was evaluated via MTT assay with human gingival fibroblasts following ISO 10993-5. Data were analyzed using one-way ANOVA and Tukey's post hoc test (\u03b1\u2009=\u20090.05).<\/jats:p>\n                  <jats:p>GO incorporation significantly increased microhardness, with G4 (0.5\u2009wt% GO) showing the highest VHN (p\u2009&lt;\u20090.05). Viscosity rose modestly with GO content, while the commercial sealant exhibited the highest value. G3 (0.25\u2009wt% GO) displayed the lowest \u0394Ra after brushing. All GO groups showed higher fluorescence than the control (p\u2009&lt;\u20090.05). Antibacterial activity increased with GO concentration (AR\u2009=\u200983.84\u201388.88%), though G5 achieved the highest AR (99.42%). All formulations maintained\u2009&gt;\u200970% cell viability, confirming acceptable biocompatibility.<\/jats:p>\n                  <jats:p>Low-level GO reinforcement enhanced surface hardness, wear resistance, fluorescence, and antibacterial performance of resin-based sealants without compromising cytocompatibility. GO represents a promising multifunctional nanofiller for next-generation sealants aimed at improving durability and caries prevention.<\/jats:p>","DOI":"10.1055\/s-0046-1819726","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T22:58:35Z","timestamp":1777071515000},"source":"Crossref","is-referenced-by-count":0,"title":["Physicomechanical and Antibacterial Properties of Resin-Based Dental Sealants Modified with Graphene Oxide Nanoparticles"],"prefix":"10.1055","author":[{"given":"Ploypim","family":"Kraisintu","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suparaksa","family":"Yamockul","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tool","family":"Sriamporn","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, College of Dental Medicine, Rangsit University, Pathum Thani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niyom","family":"Thamrongananskul","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"},{"name":"Department of Prosthodontics, Faculty of Dentistry, Burapha University, Chonburi, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8719-5119","authenticated-orcid":false,"given":"Awiruth","family":"Klaisiri","sequence":"additional","affiliation":[{"name":"Division of Restorative and Esthetic Dentistry, Faculty of Dentistry, Thammasat University, Pathum Thani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0293-765X","authenticated-orcid":false,"given":"Theerapat","family":"Chanamuangkon","sequence":"additional","affiliation":[{"name":"Biomaterial Testing Center, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somphob","family":"Thompho","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thanchanok","family":"Suriyapongprapai","sequence":"additional","affiliation":[{"name":"Dental Innovation Foundation under Royal Patronage, Wang Thonglang, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6620-1302","authenticated-orcid":false,"given":"Guang","family":"Hong","sequence":"additional","affiliation":[{"name":"Division for Globalization Initiative, Liaison Center for Innovative Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"issue":"06","key":"ref1","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.14219\/jada.archive.1973.0601","article-title":"A study of the penetration of sealants into pits and fissures","volume":"87","author":"C L Taylor","year":"1973","journal-title":"J Am Dent Assoc"},{"issue":"01","key":"ref2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.cden.2008.09.003","article-title":"Evidence-based clinical recommendations for the use of pit-and-fissure sealants: a report of 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