{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T23:38:49Z","timestamp":1777073929220,"version":"3.51.4"},"reference-count":45,"publisher":"Georg Thieme Verlag KG","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The production of porous polycaprolactone (PCL) electrospun fibers has already been reported in the literature. However, most of the studies reported the production of porous fibers on a micrometer scale. Hence, the main aim of this study is to (1) fabricate PCL membranes with secondary nanoporous fibers to improve the attachment, spreading, and proliferation of cells, and subsequent extracellular matrix deposition and (2) evaluate its biocompatibility on different cell types in comparison to a nonporous one.<\/jats:p>\n                  <jats:p>Secondary nanopores were introduced by adjusting humidity and employing a binary solvent system of chloroform and dimethyl sulfoxide. The membranes were characterized using field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD) analysis, and water contact angle. The biological activity on cells was assessed using the XTT assay, cell adhesion and spreading were analyzed by FE-SEM and confocal studies, and gene expression was measured by real-time polymerase chain reaction. Human fetal osteoblasts (hFOBs), human gingival fibroblasts (HGFs), and human umbilical vein endothelial cells (HUVECs) were utilized for cell\u2013biomaterial interaction studies.<\/jats:p>\n                  <jats:p>Two-way ANOVA (analysis of variance) followed by Tukey's multiple comparison was used to assess the statistical significance.<\/jats:p>\n                  <jats:p>The FE-SEM analysis revealed an average fiber diameter of 774\u2009\u00b1\u2009193\u2009nm for the porous membrane and 642\u2009\u00b1\u2009176\u2009nm for the nonporous membrane, with secondary nanopores averaging 151\u2009\u00b1\u200931\u2009nm in length. The XRD analyses confirmed characteristic fingerprint vibrational bands of PCL for both membrane types. Water contact angle measurement showed no significant differences, with porous membranes at 131\u2009\u00b1\u20092\u00b0 and nonporous membranes at 122\u2009\u00b1\u200910\u00b0. Both the porous and nonporous membranes displayed comparable cell viability. The porous membranes showed a tendency toward good cell spreading with statistically significant upregulation of type I collagen (COL-I) in hFOBs, and vascular endothelial growth factor (VEGF) expression in HUVECs compared with the nonporous membranes.<\/jats:p>\n                  <jats:p>The cells on porous membranes demonstrated a trend toward improved spreading and functional activity compared with those on nonporous surfaces. However, considering the exploratory nature of this work, and the lack of mechanical and functional tests, it is too early to draw a definitive conclusion on the superiority of porous PCL membranes over nonporous ones.<\/jats:p>","DOI":"10.1055\/s-0046-1820078","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T22:58:35Z","timestamp":1777071515000},"source":"Crossref","is-referenced-by-count":0,"title":["Comparative In Vitro Evaluation of Early Cell Responses of Porous and Nonporous Polycaprolactone Electrospun Nanofibrous Membrane for Guided Tissue Regeneration"],"prefix":"10.1055","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1947-253X","authenticated-orcid":false,"given":"Soumya","family":"Sheela","sequence":"additional","affiliation":[{"name":"Dental Biomaterials Research Group, Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma Mousa","family":"AlGhalban","sequence":"additional","affiliation":[{"name":"Dental Biomaterials Research Group, Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalil Abdelrazek","family":"Khalil","sequence":"additional","affiliation":[{"name":"Mechanical and Nuclear Engineering Department, College of Engineering, University of Sharjah, Sharjah, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sausan","family":"Al Kawas","sequence":"additional","affiliation":[{"name":"Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ensanya A. Abou","family":"Neel","sequence":"additional","affiliation":[{"name":"Dental Biomaterials Research Group, Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates"},{"name":"Restorative Dentistry Department, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1469414","DOI":"10.3389\/fmicb.2024.1469414","article-title":"Periodontitis: etiology, conventional treatments, and emerging bacteriophage and predatory bacteria therapies","volume":"15","author":"A \u0141asica","year":"2024","journal-title":"Front Microbiol"},{"key":"ref2","first-page":"3597303","article-title":"Corrigendum to \u201cOral Health Knowledge and Behavior among Adults in the United Arab Emirates\u201d","volume":"2019","author":"E Abu-Gharbieh","year":"2019","journal-title":"Biomed Res Int"},{"issue":"01","key":"ref3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/prd.12447","article-title":"Risk factors for periodontitis & peri-implantitis","volume":"90","author":"I Darby","year":"2022","journal-title":"Periodontol 2000"},{"issue":"01","key":"ref4","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1600-0757.2012.00457.x","article-title":"Risk factors for periodontal disease","volume":"62","author":"R J Genco","year":"2013","journal-title":"Periodontol 2000"},{"issue":"16","key":"ref5","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.3390\/polym15163355","article-title":"Guided tissue and bone regeneration membranes: a review of biomaterials and techniques for periodontal treatments","volume":"15","author":"A M Alqahtani","year":"2023","journal-title":"Polymers (Basel)"},{"issue":"03","key":"ref6","first-page":"226","article-title":"Guided tissue regeneration membranes for periodontal regeneration\u2013a literature review","volume":"11","author":"A S AlGhamdi","year":"2009","journal-title":"J Int Acad Periodontol"},{"issue":"01","key":"ref7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ijom.2013.05.017","article-title":"High-density polytetrafluoroethylene membranes in guided bone and tissue regeneration procedures: a literature review","volume":"43","author":"J M Carbonell","year":"2014","journal-title":"Int J Oral Maxillofac Implants"},{"issue":"03","key":"ref8","doi-asserted-by":"crossref","first-page":"786","DOI":"10.3390\/ma13030786","article-title":"Selection of collagen membranes for bone regeneration: a literature review","volume":"13","author":"L Sbricoli","year":"2020","journal-title":"Materials (Basel)"},{"issue":"12","key":"ref9","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1002\/polb.22259","article-title":"Biomedical applications of biodegradable polymers","volume":"49","author":"B D Ulery","year":"2011","journal-title":"J Polym Sci, B, Polym Phys"},{"issue":"08","key":"ref10","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1097\/01.don.0000152301.72828.61","article-title":"Susceptibility of a polycaprolactone-based root canal filling material to degradation. 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Alkaline hydrolysis","volume":"31","author":"F R Tay","year":"2005","journal-title":"J Endod"},{"issue":"02","key":"ref11","doi-asserted-by":"crossref","first-page":"124","DOI":"10.3390\/membranes11020124","article-title":"Bone regeneration assessment of polycaprolactone membrane on critical-size defects in rat calvaria","volume":"11","author":"A P Farnezi Bassi","year":"2021","journal-title":"Membranes (Basel)"},{"issue":"02","key":"ref12","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3390\/ma9020073","article-title":"Potential of electrospun nanofibers for biomedical and dental applications","volume":"9","author":"M Zafar","year":"2016","journal-title":"Materials (Basel)"},{"issue":"01","key":"ref13","doi-asserted-by":"crossref","first-page":"16","DOI":"10.3390\/nano10010016","article-title":"A critical review on the production of electrospun nanofibres for guided bone regeneration in oral surgery","volume":"10","author":"F 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functionalized with ibuprofen for periodontal regeneration: an in vitro and in vivo study","volume":"11","author":"F Batool","year":"2018","journal-title":"Materials (Basel)"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"108915","DOI":"10.1016\/j.matdes.2020.108915","article-title":"Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration","volume":"194","author":"S Metwally","year":"2020","journal-title":"Mater Des"},{"issue":"09","key":"ref29","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1016\/j.dental.2015.06.004","article-title":"Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration","volume":"31","author":"E A M\u00fcnchow","year":"2015","journal-title":"Dent Mater"},{"issue":"08","key":"ref30","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1021\/ie50320a024","article-title":"RESISTANCE OF SOLID SURFACES TO WETTING BY WATER","volume":"28","author":"R N Wenzel","year":"1936","journal-title":"Ind Eng Chem"},{"issue":"01","key":"ref31","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3390\/ma9010057","article-title":"Advanced engineering strategies for periodontal complex regeneration","volume":"9","author":"C H Park","year":"2016","journal-title":"Materials (Basel)"},{"issue":"04","key":"ref32","first-page":"1175","article-title":"Dimethyloxallyl glycine\/nanosilicates-loaded osteogenic\/angiogenic difunctional fibrous structure for functional periodontal tissue regeneration","volume":"6","author":"L Shang","year":"2020","journal-title":"Bioact Mater"},{"issue":"21","key":"ref33","doi-asserted-by":"crossref","first-page":"11655","DOI":"10.3390\/app152111655","article-title":"Nucleic acid-based therapeutics for periodontal tissue regeneration: a comprehensive review","volume":"15","author":"J Y Kim","year":"2025","journal-title":"Appl Sci 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Rep"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"137954","DOI":"10.1016\/j.ijbiomac.2024.137954","article-title":"Electrospinning of recombinant human-like collagen-reinforced PCL nanofibrous membranes using benign solvents for periodontal regeneration","volume":"284","author":"M Ren","year":"2025","journal-title":"Int J Biol Macromol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"670010","DOI":"10.3389\/fmats.2021.670010","article-title":"Polyblend nanofibers to regenerate gingival tissue: a preliminary in vitro study","volume":"8","author":"E Canciani","year":"2021","journal-title":"Front Mater"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"219","DOI":"10.2174\/1874210600903010219","article-title":"Effects of aging and cyclosporin a on collagen turnover in human gingiva","volume":"3","author":"N Gagliano","year":"2009","journal-title":"Open Dent 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