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Sensor signals are electronically processed several ways for optimum cell discrimination and are displayed as pulse-amplitude distributions using a pulse-height analyzer. Processed signals trigger cell sorting according to preselected parametric criteria. Sorting is accomplished by electrically charging droplets containing the cells and electrostatically deflecting them into collection vessels. This instrument is described in detail with illustrative examples of experiments using polystyrene fluorescent microspheres, cultured human cells, and human leukocytes.<\/jats:p>","DOI":"10.1063\/1.1686375","type":"journal-article","created":{"date-parts":[[2004,2,10]],"date-time":"2004-02-10T18:02:04Z","timestamp":1076436124000},"page":"1301-1310","update-policy":"http:\/\/dx.doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":151,"title":["A New Multiparameter Separator for Microscopic Particles and Biological Cells"],"prefix":"10.1063","volume":"44","author":[{"given":"J. A.","family":"Steinkamp","sequence":"first","affiliation":[{"name":"Biophysics and Instrumentation Group, Los Alamos Scientific Laboratory, University of California, Los Alamos, New Mexico 87544"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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