{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T06:30:36Z","timestamp":1779258636459,"version":"3.51.4"},"reference-count":46,"publisher":"AIP Publishing","issue":"2","content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,6,1]]},"abstract":"<jats:p>Enzyme-linked immunosorbent assay (ELISA) has been widely used in medical diagnostics, environmental analyses, and biochemical studies. To reduce assay time and lower consumption of reagents in cytokine ELISA analysis, a polymeric microfluidic biochip has been designed and fabricated via several new techniques: Polyaniline-based surface modification for superhydrophobic capillary valving and oxygen plasma-poly(ethyleneimine)-tyrosinase-protein A modification for high sensitivity protein detection. The proper flow sequencing was achieved using the superhydrophobic capillary valves. The burst frequency of each valve was experimentally determined and compared with two capillary force equations and the fluent finite element simulation. This fully automated microfluidic biochip with an analyzer is able to provide high fluorescence signal of ELISA with a wider linear detection range and a much shorter assay time than 96-well microtiter plates. It is applicable to a variety of nonclinic research and clinically relevant disease conditions. The modification technologies in this study can be implemented in other lab-on-a-chip systems, drug\/gene delivery carriers, and other immunoassay biosensor applications.<\/jats:p>","DOI":"10.1063\/1.3116665","type":"journal-article","created":{"date-parts":[[2009,4,13]],"date-time":"2009-04-13T22:59:12Z","timestamp":1239663552000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Design and testing of a microfluidic biochip for cytokine enzyme-linked immunosorbent assay"],"prefix":"10.1063","volume":"3","author":[{"given":"Hongyan","family":"He","sequence":"first","affiliation":[{"name":"The Ohio State University 1 Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, , Columbus, Ohio 43210, USA"},{"name":"LLC 2 BioLOC, , 1381 Kinnear Road Suite 100, Columbus, Ohio 43212, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Yuan","sequence":"additional","affiliation":[{"name":"The Ohio State University 1 Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, , Columbus, Ohio 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weixiong","family":"Wang","sequence":"additional","affiliation":[{"name":"The Ohio State University 1 Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, , Columbus, Ohio 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan-Rong","family":"Chiou","sequence":"additional","affiliation":[{"name":"Nanomaterial Innovation Ltd. 3 , 3390 Green River Dr., Columbus, Ohio 43228, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur J.","family":"Epstein","sequence":"additional","affiliation":[{"name":"The Ohio State University 4 Department of Physics, , Columbus, Ohio 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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