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Both two-dimensional (ring-patterned) and three-dimensional cell aggregates were produced. The cell aggregates were maintained under sterile conditions at 37\u2009\u00b0C for up to 14 days by continuously flushing Sauton\u2019s medium through the chamber. Resuscitation of dormant M. smegmatis was evaluated by the production of the fluorescent dye 5-cyano-2,3-ditolyltetrazolium chloride. The results confirm that the resuscitation of dormant M. smegmatis is triggered by the accumulation of a resuscitation promoting factor inside the aggregates by diffusion limitation.<\/jats:p>","DOI":"10.1063\/1.3435336","type":"journal-article","created":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T22:21:23Z","timestamp":1277850083000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Construction by dielectrophoresis of microbial aggregates for the study of bacterial cell dormancy"],"prefix":"10.1063","volume":"4","author":[{"given":"Ke","family":"Zhu","sequence":"first","affiliation":[{"name":"The University of Manchester 1 School of Chemical and Analytical Science, , Manchester M60 1Qd, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arseny S.","family":"Kaprelyants","sequence":"additional","affiliation":[{"name":"Russian Academy of Sciences 2 Bach Institute of Biochemistry, , Moscow 117071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena G.","family":"Salina","sequence":"additional","affiliation":[{"name":"Russian Academy of Sciences 2 Bach Institute of Biochemistry, , Moscow 117071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Schuler","sequence":"additional","affiliation":[{"name":"University of Stuttgart 3 Institute of Biochemical Engineering, , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard H.","family":"Markx","sequence":"additional","affiliation":[{"name":"Heriot-Watt University 4 Department of Chemical Engineering, , Edinburgh, Scotland EH14 4AS, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"317","published-online":{"date-parts":[[2010,6,29]]},"reference":[{"key":"2023070517362466800_c1","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1177\/014107680109400813","volume":"94","year":"2001","journal-title":"J. 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