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We developed a microfluidics platform for efficient trapping and immobilization of enteroids for quantitative measurement of volume changes. Multiple enteroids are trapped in a \u201cpinball machine-like\u201d array of polydimethylsiloxane posts for measurement of volume changes in unlabeled enteroids by imaging of an extracellular, high-molecular weight fluorescent dye. Measurement accuracy was validated using slowly expanding air bubbles. The method was applied to measure swelling of mouse jejunal enteroids in response to an osmotic challenge and cholera toxin-induced chloride secretion. The microfluidics platform allows for parallel measurement of volume changes on multiple enteroids during continuous superfusion, without an immobilizing matrix, and for quantitative volume determination without chemical labeling or assumptions about enteroid shape changes during swelling.<\/jats:p>","DOI":"10.1063\/1.4870400","type":"journal-article","created":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T00:30:23Z","timestamp":1396398623000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Microfluidics platform for measurement of volume changes in immobilized intestinal enteroids"],"prefix":"10.1063","volume":"8","author":[{"given":"Byung-Ju","family":"Jin","sequence":"first","affiliation":[{"name":"Departments of Medicine and Physiology, University of California 1 , San Francisco, California 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sailaja","family":"Battula","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology, University of California 1 , San Francisco, California 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"Zachos","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine 2 , Baltimore, Maryland 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Kovbasnjuk","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine 2 , Baltimore, Maryland 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Fawlke-Abel","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine 2 , Baltimore, Maryland 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"In","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine 2 , Baltimore, Maryland 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Donowitz","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine 2 , Baltimore, Maryland 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan S.","family":"Verkman","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology, University of California 1 , San Francisco, California 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"317","published-online":{"date-parts":[[2014,4,1]]},"reference":[{"key":"2023070517490870000_c1","doi-asserted-by":"crossref","first-page":"C1109","DOI":"10.1152\/ajpcell.1997.273.4.C1109","volume":"273","year":"1997","journal-title":"Am. 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