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We demonstrate control over perfusion rates in the 10-\u03bcl\/s range and the ability to change between fluid reservoirs at up to 50 MPa. We validate the screw-pump approach by monitoring the cyanide-induced response of UV-excited autofluorescence from Saccharomyces cerevisiae under pressurization.<\/jats:p>","DOI":"10.1063\/1.4899121","type":"journal-article","created":{"date-parts":[[2014,10,25]],"date-time":"2014-10-25T00:39:11Z","timestamp":1414197551000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Note: A micro-perfusion system for use during real-time physiological studies under high pressure"],"prefix":"10.1063","volume":"85","author":[{"given":"Jeff","family":"Maltas","sequence":"first","affiliation":[{"name":"Miami University Department of Physics, , Oxford, Ohio 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zac","family":"Long","sequence":"additional","affiliation":[{"name":"Miami University Department of Physics, , Oxford, Ohio 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison","family":"Huff","sequence":"additional","affiliation":[{"name":"Miami University Department of Physics, , Oxford, Ohio 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Maloney","sequence":"additional","affiliation":[{"name":"Miami University Department of Physics, , Oxford, Ohio 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordan","family":"Ryan","sequence":"additional","affiliation":[{"name":"Miami University Department of Physics, , Oxford, Ohio 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Urayama","sequence":"additional","affiliation":[{"name":"Miami University Department of Physics, , Oxford, Ohio 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"317","published-online":{"date-parts":[[2014,10,24]]},"reference":[{"key":"2023070415560094400_c1","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.jbiosc.2011.09.017","volume":"113","year":"2012","journal-title":"J. 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