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In this paper, we present a low-cost universal serial bus (USB)-interfaced mobile platform to perform microfluidic ELISA operations in detecting the presence and concentrations of BDE-47 (2,2\u2032,4,4\u2032-tetrabromodiphenyl ether), an environmental contaminant found in our food supply with adverse health impact. Our point-of-care diagnostic device utilizes flexible interdigitated carbon black electrodes to convert electric current into a microfluidic pump via gas bubble expansion during electrolytic reaction. The micropump receives power from a mobile phone and transports BDE-47 analytes through the microfluidic device conducting competitive ELISA. Using variable domain of heavy chain antibodies (commonly referred to as single domain antibodies or Nanobodies), the proposed device is sensitive for a BDE-47 concentration range of 10\u22123\u2013104\u2009\u03bcg\/l, with a comparable performance to that uses a standard competitive ELISA protocol. It is anticipated that the potential impact in mobile detection of health and environmental contaminants will prove beneficial to our community and low-resource environments.<\/jats:p>","DOI":"10.1063\/1.4901348","type":"journal-article","created":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T01:30:42Z","timestamp":1415237442000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay"],"prefix":"10.1063","volume":"8","author":[{"given":"Arnold","family":"Chen","sequence":"first","affiliation":[{"name":"University of California 1 Department of Biomedical Engineering, , Davis, California 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Royal","family":"Wang","sequence":"additional","affiliation":[{"name":"University of California 1 Department of Biomedical Engineering, , Davis, California 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candace R. 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