{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T13:22:47Z","timestamp":1778764967754,"version":"3.51.4"},"reference-count":41,"publisher":"AIP Publishing","issue":"2","funder":[{"name":"China Major New Drugs Development Program","award":["2014ZX09507008"],"award-info":[{"award-number":["2014ZX09507008"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771088"],"award-info":[{"award-number":["31771088"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81301293"],"award-info":[{"award-number":["81301293"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,3,1]]},"abstract":"<jats:p>Portable Polymerase Chain Reaction (PCR) devices combined with microfluidic chips or lateral flow stripes have shown great potential in the field of point-of-need testing (PoNT) as they only require a small volume of patient sample and are capable of presenting results in a short time. However, the detection for multiple targets in this field leaves much to be desired. Herein, we introduce a novel PCR platform by integrating a bottom-well microfluidic chip with an infra-red (IR) excited temperature control method and fluorescence co-detection of three PCR products. Microfluidic chips are utilized to partition different samples into individual bottom-wells. The oil phase in the main channel contains multi-walled carbon nanotubes which were used as a heat transfer medium that absorbs energy from the IR-light-emitting diode (LED) and transfers heat to the water phase below. Cyclical rapid heating and cooling necessary for PCR are achieved by alternative power switching of the IR-LED and Universal Serial Bus (USB) mini-fan with a pulse width modulation scheme. This design of the IR-LED PCR platform is economic, compact, and fully portable, making it a promising application in the field of PoNT. The bottom-well microfluidic chip and IR-LED PCR platform were combined to fulfill a three-stage thermal cycling PCR for 40 cycles within 90\u2009min for Human Papilloma Virus (HPV) detection. The PCR fluorescent signal was successfully captured at the end of each cycle. The technique introduced here has broad applications in nucleic acid amplification and PoNT devices.<\/jats:p>","DOI":"10.1063\/1.5023652","type":"journal-article","created":{"date-parts":[[2018,3,14]],"date-time":"2018-03-14T16:12:53Z","timestamp":1521043973000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Simultaneous detection of multiple HPV DNA via bottom-well microfluidic chip within an infra-red PCR platform"],"prefix":"10.1063","volume":"12","author":[{"given":"Wenjia","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antony","family":"Warden","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahui","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangxia","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianting","family":"Ding","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"317","published-online":{"date-parts":[[2018,3,14]]},"reference":[{"key":"2023070517323583300_c1","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.bios.2016.11.063","article-title":"An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics","volume":"91","year":"2017","journal-title":"Biosens. 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