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However, commonly used additives such as Pluronic F-68 and other poloxamer macromolecules can change the mechanical properties of cells and consequently alter their processing. In this paper, we quantified the short-term effect of Pluronic F-68 on the mechanotype of three different cell types (Jurkat cells, red blood cells, and human embryonic kidney cells) using real-time deformability cytometry. The impact of the additive concentration was assessed in terms of cell size and deformability. We observed that cells respond progressively to the presence of Pluronic F-68 within first 3 h of incubation and become significantly stiffer (p-value &amp;lt; 0.001) in comparison to a serum-free control and a control containing serum. We also observed that the short-term response manifested as cell stiffening is true (p-value &amp;lt; 0.001) for the concentration reaching 1% (w\/v) of the poloxamer additive in tested buffers. Additionally, using flow cytometry, we assessed that changes in cell deformability triggered by addition of Pluronic F-68 are not accompanied by size or viability alterations.<\/jats:p>","DOI":"10.1063\/1.5040316","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T10:43:14Z","timestamp":1534934594000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry"],"prefix":"10.1063","volume":"12","author":[{"given":"Ewa","family":"Guzniczak","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Science, Heriot-Watt University, Edinburgh Campus 1 , Edinburgh EH14 4AS, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4631-0608","authenticated-orcid":false,"given":"Melanie","family":"Jimenez","sequence":"additional","affiliation":[{"name":"School of Engineering, Biomedical Engineering Division, University of Glasgow 2 , Glasgow G12 8LT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Irwin","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Science, Heriot-Watt University, Edinburgh Campus 1 , Edinburgh EH14 4AS, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0280-5374","authenticated-orcid":false,"given":"Oliver","family":"Otto","sequence":"additional","affiliation":[{"name":"ZIK HIKE, Centre for Innovation Competence - Humoral Immune Reactions in Cardiovascular Diseases, Biomechanics, University of Greifswald 3 , Fleischmannstra\u00dfe 42-44, 17489 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Willoughby","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Science, Heriot-Watt University, Edinburgh Campus 1 , Edinburgh EH14 4AS, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Bridle","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Science, Heriot-Watt University, Edinburgh Campus 1 , Edinburgh EH14 4AS, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"317","published-online":{"date-parts":[[2018,8,22]]},"reference":[{"issue":"3","key":"2023070517354402000_c1","doi-asserted-by":"publisher","first-page":"449","DOI":"10.3390\/s17030449","article-title":"Dielectrophoresis for biomedical sciences applications: A review","volume":"17","year":"2017","journal-title":"Sensors (Basel)"},{"issue":"1","key":"2023070517354402000_c2","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.stem.2008.11.012","article-title":"Isolation and production of cells suitable for human therapy: Challenges ahead","volume":"4","year":"2009","journal-title":"Cell Stem Cell"},{"issue":"1","key":"2023070517354402000_c3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0927-7757(94)03028-X","article-title":"Poly(ethylene oxide)\u00b7poly(propylene oxide)\u00b7poly(ethylene oxide) block copolymer surfactants in aqueous solutions and at interfaces: Thermodynamics, structure, dynamics, and modeling","volume":"96","year":"1995","journal-title":"Colloids Surf. 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