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Through the integration of SU-8 negative photoresist as a microchannel and the precise alignment of the SU-8 microchannel with the on-chip gold interdigitated microelectrodes, bacteria trapping efficiencies of up to 97.4%, 97.7%, and 37.7% were achieved for E. coli, V. cholerae, and Enterococcus, respectively. The DEPIM device enables a high detection sensitivity, which requires only a total number of 69\u2009\u00b1\u200933 E. coli cells, 9\u2009\u00b1\u20092 Vibrio cholera cells, and 36\u2009\u00b1\u200913 Enterococcus cells to observe a discernible change in system impedance for detection. Nonetheless, the corrected limit of detection for Enterococcus is 95\u2009\u00b1\u200934 after taking into consideration the lower trapping efficiency. In addition, a theoretical model is developed to allow for the direct estimation of the number of bacteria through a linear relationship with the change in the reciprocal of the overall system absolute impedance.<\/jats:p>","DOI":"10.1063\/5.0024826","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T12:30:15Z","timestamp":1602678615000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Dielectrophoretic trapping and impedance detection of <i>Escherichia coli<\/i>, <i>Vibrio cholera<\/i>, and <i>Enterococci<\/i> bacteria"],"prefix":"10.1063","volume":"14","author":[{"given":"Tian Fook","family":"Kong","sequence":"first","affiliation":[{"name":"School of Mechanical and Aerospace Engineering, Nanyang Technological University 1 , 50 Nanyang Avenue, Singapore 639798"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhui","family":"Shen","sequence":"additional","affiliation":[{"name":"School of Mechanical and Aerospace Engineering, Nanyang Technological University 1 , 50 Nanyang Avenue, Singapore 639798"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5657-196X","authenticated-orcid":false,"family":"Marcos","sequence":"additional","affiliation":[{"name":"School of Mechanical and Aerospace Engineering, Nanyang Technological University 1 , 50 Nanyang Avenue, Singapore 639798"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1191-7642","authenticated-orcid":false,"given":"Chun","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Mechanical and Aerospace Engineering, Nanyang Technological University 1 , 50 Nanyang Avenue, Singapore 639798"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imran Halimi","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"DNV GL Technology Centre, DNV GL 2 , 16 Science Park Drive, Singapore 118227"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"317","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"issue":"6","key":"2023062410290475400_c1","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1111\/ele.12111","volume":"16","year":"2013","journal-title":"Ecol. 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