{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:49:18Z","timestamp":1790707758497,"version":"4.1.0"},"reference-count":49,"publisher":"AIP Publishing","issue":"1","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:p>Diabetes is a chronic degenerative disease that carries multiple complications. One of the most important complications is the diabetic cutaneous complications, such as skin lesions, ulcerations, and diabetic foot, which are present in 30%\u201370% of the patients. Currently, the treatments for wound healing include growth factors and cytokines, skin substitutes, hyperbaric oxygen therapy, and skin grafts. However, these treatments are ineffective due to the complex mechanisms involved in developing unhealed wounds. Considering the aforementioned complications, regenerative medicine has focused on this pathology using stem cells to improve these complications. However, it is essential to mention that there is a poor biomolecular understanding of diabetic skin and the effects of treating it with stem cells. For this reason, herein, we investigated the employment of pluripotent stem cells (PSC) in the wound healing process by carrying out morphometric, histological, and Fourier-transform infrared microspectroscopy (FTIRM) analysis. The morphometric analysis was done through a photographic follow-up, measuring the lesion areas. For the histological analysis, hematoxylin &amp; eosin and picrosirius red stains were used to examine the thickness of the epidermis and the cellularity index in the dermis as well as the content and arrangement of collagen type I and III fibers. Finally, for the FTIRM analysis, skin cryosections were obtained and analyzed by employing a Cassegrain objective of 16\u00d7 of an FTIR microscope coupled to an FTIR spectrometer. For this purpose, 20 mice were divided into two groups according to the treatment they received: the Isotonic Salt Solution (ISS) group and the PSCs group (n\u2009=\u200910). Both groups were induced to diabetes, and six days after diabetes induction, an excisional lesion was made in the dorsal area. Furthermore, using microscopy and FTIRM analysis, the skin healing process on days 7 and 15 post-skin lesion excision was examined. The results showed that the wound healing process over time, considering the lesion size, was similar in both groups; however, the PSCs group evidenced hair follicles in the wound. Moreover, the histological analysis evidenced that the PSCs group exhibited granulation tissue, new vessels, and better polarity of the keratinocytes. In addition, the amount of collagen increased with a good deposition and orientation, highlighting that type III collagen fibers were more abundant in the PSCs. Finally, the FTIR analysis evidenced that the PSCs group exhibited a faster wound healing process. In conclusion, the wounds treated with PSCs showed a more rapid wound healing process, less inflammatory cellular infiltration, and more ordered structures than the ISS group.<\/jats:p>","DOI":"10.1063\/5.0130383","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T12:00:39Z","timestamp":1675771239000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Skin wound healing improvement in diabetic mice through FTIR microspectroscopy after implanting pluripotent stem cells"],"prefix":"10.1063","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6657-0722","authenticated-orcid":false,"given":"Gustavo J.","family":"Vazquez-Zapien","sequence":"first","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"},{"name":"Centro de Investigaci\u00f3n y Desarrollo del Ej\u00e9rcito y Fuerza A\u00e9rea Mexicanos, Secretar\u00eda de la Defensa Nacional 2 , Mexico City 11400, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1849-5190","authenticated-orcid":false,"given":"Adriana","family":"Martinez-Cuazitl","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"},{"name":"Escuela Nacional de Medicina y Homeopat\u00eda, Instituto Polit\u00e9cnico Nacional 3 , Mexico City 07320, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8651-6742","authenticated-orcid":false,"given":"Alejandra","family":"Granados-Jimenez","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4371-0486","authenticated-orcid":false,"given":"Miguel","family":"Sanchez-Brito","sequence":"additional","affiliation":[{"name":"Escuela Superior de C\u00f3mputo, Instituto Polit\u00e9cnico Nacional 4 , Mexico City 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8078-3353","authenticated-orcid":false,"given":"Melissa","family":"Guerrero-Ruiz","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Camacho-Ibarra","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6042-5401","authenticated-orcid":false,"given":"Misael A.","family":"Miranda-Ruiz","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6996-2326","authenticated-orcid":false,"given":"Ian S.","family":"Dox-Aguill\u00f3n","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus A.","family":"Ramirez-Torres","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1811-4511","authenticated-orcid":false,"given":"Monica M.","family":"Mata-Miranda","sequence":"additional","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretar\u00eda de la Defensa Nacional 1 , Mexico City 11200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"317","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"2023081023290839500_c1","unstructured":"See https:\/\/www.diabetesatlas.org for \u201cIDF Diabetes Atlas, Ninth edition,\u201d 2019 (last accessed September, 2021)."},{"key":"2023081023290839500_c2","doi-asserted-by":"publisher","first-page":"100037","DOI":"10.1016\/j.mbplus.2020.100037","article-title":"The role of extracellular matrix in the pathophysiology of diabetic wounds","volume":"6\u20137","year":"2020","journal-title":"Matrix Biol. 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