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The strength of depletion interactions depends on physical parameters such as the depletant number density and the depletant size ratio. Cells are known to dynamically regulate these two parameters by varying the copy number of proteins of a wide distribution of sizes. However, mammalian cells are also known to keep the total protein mass density remarkably constant, to within 0.5% throughout the cell cycle. We thus ask how the strength of depletion interactions varies when the total depletant mass is held fixed, a.k.a. fixed-mass depletion. We answer this question via scaling arguments, as well as by studying depletion effects on networks of reconstituted semiflexible actin in silico and in vitro. We examine the maximum strength of the depletion interaction potential U\u2217 as a function of q, the size ratio between the depletant and the matter being depleted. We uncover a scaling relation U\u2217 \u223c q\u03b6 for two cases: fixed volume fraction \u03c6 and fixed mass density \u03c1. For fixed volume fraction, we report \u03b6 &amp;lt; 0. For the fixed mass density case, we report \u03b6 &amp;gt; 0, which suggests that the depletion interaction strength increases as the depletant size ratio is increased. To test this prediction, we prepared our filament networks at fixed mass concentrations with varying sizes of the depletant molecule poly(ethylene glycol) (PEG). We characterize the depletion interaction strength in our simulations via the mesh size. In experiments, we observe two distinct actin network morphologies, which we call weakly bundled and strongly bundled. We identify a mass concentration where different PEG depletant sizes lead to weakly bundled or strongly bundled morphologies. For these conditions, we find that the mesh size and intra-bundle spacing between filaments across the different morphologies do not show significant differences, while the dynamic light scattering relaxation time and storage modulus between the two states do show significant differences. Our results demonstrate the ability to tune actin network morphology and mechanics by controlling depletant size and give insights into depletion interaction mechanisms under the fixed-depletant-mass constraint relevant to living cells.<\/jats:p>","DOI":"10.1063\/5.0197269","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:12:20Z","timestamp":1724285540000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Morphological control of bundled actin networks subject to fixed-mass depletion"],"prefix":"10.1063","volume":"161","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2423-2916","authenticated-orcid":false,"given":"James","family":"Clarke","sequence":"first","affiliation":[{"name":"UT Austin Department of Physics 1 , 2515 Speedway, Austin, Texas 78712, 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