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We present an innovative single-cell printing chip that utilizes thermal inkjet technology for single-cell printing, therefore addressing these constraints. We have accomplished high-throughput, wide-field, and efficient single-cell printing by merging a high-density thermal foam-based inkjet nozzle array on a chip with high-speed cameras and computer vision technologies for optical image capture and single-cell identification training. We have shown the efficacy and adaptability of the printing chip by printing various concentrations of Chinese hamster ovary cells and human embryonic kidney 293 cells. The printing of a single 96-well plate is accomplished in 2\u20133\u2009min, facilitating one-time loading and uninterrupted multi-plate paving. Our thermal bubble single-cell printing chip serves as a viable platform for high-throughput single-cell analysis applications.<\/jats:p>","DOI":"10.1063\/5.0225883","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T19:23:20Z","timestamp":1732649000000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Thermal bubble single-cell printing chip: High-throughput, wide-field, and efficient"],"prefix":"10.1063","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8426-2365","authenticated-orcid":false,"given":"Bo","family":"Deng","sequence":"first","affiliation":[{"name":"School of Microelectronics, Shanghai University 1 , Shanghai 201800,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1904-2186","authenticated-orcid":false,"given":"Kun","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Aurefluidics Technology Co. 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