{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:30:11Z","timestamp":1790861411267,"version":"4.1.0"},"reference-count":106,"publisher":"AIP Publishing","issue":"4","content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,12,1]]},"abstract":"<jats:p>Biomolecular assemblies form via intramolecular interactions and serve important biological functions. The most characterized biomolecular assemblies are amyloid fibrils, which are associated with neurodegenerative diseases. Advances in microscopy techniques enabled characterization of the morphology of these assemblies, but so far, failed in detailed structural characterizations. Vibrational spectroscopic imaging presents unique advantages to studying biomolecular assemblies in their natural environment due to the sensitivity of vibrational spectra to protein structural changes, especially \u03b2-sheet enrichment in amyloid fibrils. High-resolution hyperspectral images originating from distinct vibrations of chemical bonds provide label-free characterizations of biomolecules, including proteins, lipids, and nucleic acids. In this review, we first briefly introduce infrared and Raman-based spectroscopy and their biological interpretation. We then review applications adopting Fourier transform Infrared-based, mid-infrared photothermal-based, and Raman-based approaches in tissue and cells, especially live cells. Finally, we discuss how these technologies are evolving to study biomolecular assemblies beyond amyloid fibrils.<\/jats:p>","DOI":"10.1063\/5.0244025","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T13:48:39Z","timestamp":1762436919000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Feeling the vibes: Vibrational spectroscopic imaging of biomolecular assemblies in their natural environment"],"prefix":"10.1063","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0780-186X","authenticated-orcid":false,"given":"Zhongyue","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Boston University 1 , Boston, Massachusetts 02215,","place":["USA"]},{"name":"Photonics Center, Boston University 2 , Boston, Massachusetts 02215,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7077-5934","authenticated-orcid":false,"given":"Giulio","family":"Chiesa","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Toronto 3 , Toronto, Ontario M5S 3E2,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5607-6683","authenticated-orcid":false,"given":"Ji-Xin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Boston University 1 , Boston, Massachusetts 02215,","place":["USA"]},{"name":"Photonics Center, Boston University 2 , Boston, Massachusetts 02215,","place":["USA"]},{"name":"Department of Electrical and Computer Engineering, Boston University 4 , Boston, Massachusetts 02215,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"317","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"issue":"3","key":"2025110608483190900_c1","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1038\/s41580-020-00326-6","article-title":"Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing","volume":"22","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2025110608483190900_c2","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/bs.ircmb.2016.08.014","article-title":"The nucleation of protein aggregates-from crystals to amyloid fibrils","volume":"329","year":"2017","journal-title":"Int. Rev. Cell Mol. Biol."},{"issue":"1","key":"2025110608483190900_c3","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1146\/annurev-genet-120215-034943","article-title":"Proteopathic strains and the heterogeneity of neurodegenerative diseases","volume":"50","year":"2016","journal-title":"Annu. Rev. Genet."},{"issue":"4","key":"2025110608483190900_c4","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1016\/j.cell.2022.12.032","article-title":"Hallmarks of neurodegenerative diseases","volume":"186","year":"2023","journal-title":"Cell"},{"issue":"8","key":"2025110608483190900_c5","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1038\/ni.2913","article-title":"The adaptor ASC has extracellular and \u2018prionoid\u2019 activities that propagate inflammation","volume":"15","year":"2014","journal-title":"Nat. Immunol."},{"issue":"12","key":"2025110608483190900_c6","doi-asserted-by":"publisher","first-page":"1722","DOI":"10.1016\/j.str.2014.09.011","article-title":"Structural and dynamics aspects of ASC speck assembly","volume":"22","year":"2014","journal-title":"Structure"},{"issue":"5938","key":"2025110608483190900_c7","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1126\/science.1173155","article-title":"Functional amyloids as natural storage of peptide hormones in pituitary secretory granules","volume":"325","year":"2009","journal-title":"Science"},{"issue":"1","key":"2025110608483190900_c8","doi-asserted-by":"publisher","first-page":"e02279-18","DOI":"10.1128\/mBio.02279-18","article-title":"Physical determinants of amyloid assembly in biofilm formation","volume":"10","year":"2019","journal-title":"mBio"},{"issue":"1","key":"2025110608483190900_c9","doi-asserted-by":"publisher","first-page":"2822","DOI":"10.1038\/s41467-023-38204-2","article-title":"Structural analysis and architectural principles of the bacterial amyloid curli","volume":"14","year":"2023","journal-title":"Nat. Commun."},{"key":"2025110608483190900_c10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12964-015-0125-7","article-title":"Phase separation in biology; functional organization of a higher order","volume":"14","year":"2016","journal-title":"Cell Commun. Signal."},{"issue":"7","key":"2025110608483190900_c11","doi-asserted-by":"publisher","first-page":"1276","DOI":"10.1016\/j.bpj.2021.02.008","article-title":"Real-time observation of structure and dynamics during the liquid-to-solid transition of FUS LC","volume":"120","year":"2021","journal-title":"Biophys. J."},{"key":"2025110608483190900_c12","doi-asserted-by":"publisher","first-page":"e202408163","DOI":"10.1002\/ange.202408163","article-title":"Structural mapping of protein aggregates in live cells modeling Huntington's disease","volume":"136","year":"2024","journal-title":"Angew. Chem., Int. Ed."},{"issue":"22","key":"2025110608483190900_c13","doi-asserted-by":"publisher","first-page":"4290","DOI":"10.1016\/j.molcel.2022.09.031","article-title":"Formation of toxic oligomers of polyQ-expanded Huntingtin by prion-mediated cross-seeding","volume":"82","year":"2022","journal-title":"Mol. Cell"},{"issue":"5","key":"2025110608483190900_c14","doi-asserted-by":"publisher","first-page":"e201900489","DOI":"10.26508\/lsa.201900489","article-title":"The Huntingtin inclusion is a dynamic phase-separated compartment","volume":"2","year":"2019","journal-title":"Life Sci. Alliance"},{"issue":"10","key":"2025110608483190900_c15","doi-asserted-by":"publisher","first-page":"1340","DOI":"10.1038\/s41557-023-01289-9","article-title":"The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils","volume":"15","year":"2023","journal-title":"Nat. Chem."},{"issue":"3","key":"2025110608483190900_c16","doi-asserted-by":"publisher","first-page":"102926","DOI":"10.1016\/j.jbc.2023.102926","article-title":"Liquid\u2013liquid phase separation of amyloid-\u03b2 oligomers modulates amyloid fibrils formation","volume":"299","year":"2023","journal-title":"J. Biol. Chem."},{"issue":"12","key":"2025110608483190900_c17","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1038\/s41580-018-0060-8","article-title":"A new era for understanding amyloid structures and disease","volume":"19","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2025110608483190900_c18","doi-asserted-by":"publisher","first-page":"e202400001","DOI":"10.1002\/anse.202400001","article-title":"Visualizing amyloid assembly at the nanoscale: Insights from super-resolution imaging","volume":"4","year":"2024","journal-title":"Anal. Sens."},{"issue":"1","key":"2025110608483190900_c19","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/nan.12646","article-title":"STochastic optical reconstruction microscopy (STORM) reveals the nanoscale organization of pathological aggregates in human brain","volume":"47","year":"2021","journal-title":"Neuropathol. Appl. Neurobiol."},{"issue":"18","key":"2025110608483190900_c20","doi-asserted-by":"publisher","first-page":"9412","DOI":"10.1021\/acs.chemrev.7b00767","article-title":"Switchable fluorophores for single-molecule localization microscopy","volume":"118","year":"2018","journal-title":"Chem. Rev."},{"issue":"1","key":"2025110608483190900_c21","doi-asserted-by":"publisher","first-page":"4155","DOI":"10.1038\/s41467-023-39776-9","article-title":"Structural and photophysical characterization of the small ultra-red fluorescent protein","volume":"14","year":"2023","journal-title":"Nat. Commun."},{"issue":"5","key":"2025110608483190900_c22","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1038\/nmeth.1596","article-title":"Two-photon absorption properties of fluorescent proteins","volume":"8","year":"2011","journal-title":"Nat. Methods"},{"issue":"1","key":"2025110608483190900_c23","doi-asserted-by":"publisher","first-page":"12819","DOI":"10.1038\/s41598-021-92249-1","article-title":"Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research","volume":"11","year":"2021","journal-title":"Sci. Rep."},{"issue":"6","key":"2025110608483190900_c24","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1016\/j.chembiol.2014.03.014","article-title":"Direct observations of amyloid \u03b2 self-assembly in live cells provide insights into differences in the kinetics of A\u03b2 (1\u201340) and A\u03b2 (1\u201342) aggregation","volume":"21","year":"2014","journal-title":"Chem. Biol."},{"issue":"7980","key":"2025110608483190900_c25","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1038\/s41586-023-06437-2","article-title":"Molecular pathology of neurodegenerative diseases by cryo-EM of amyloids","volume":"621","year":"2023","journal-title":"Nature"},{"key":"2025110608483190900_c26","doi-asserted-by":"publisher","first-page":"16706","DOI":"10.1039\/D4NR01877J","article-title":"Revealing nanoscale structure and interfaces of protein and polymer condensates via cryo-electron microscopy","volume":"16","year":"2024","journal-title":"Nanoscale"},{"issue":"6264","key":"2025110608483190900_c27","doi-asserted-by":"publisher","first-page":"aaa8870","DOI":"10.1126\/science.aaa8870","article-title":"Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine","volume":"350","year":"2015","journal-title":"Science"},{"issue":"9","key":"2025110608483190900_c28","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1016\/j.bbabio.2007.06.004","article-title":"Infrared spectroscopy of proteins","volume":"1767","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"issue":"8","key":"2025110608483190900_c29","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1111\/j.1745-7270.2007.00320.x","article-title":"Fourier transform infrared spectroscopic analysis of protein secondary structures","volume":"39","year":"2007","journal-title":"Acta Biochim. Biophys. Sin."},{"issue":"3","key":"2025110608483190900_c30","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1038\/nprot.2015.024","article-title":"Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy","volume":"10","year":"2015","journal-title":"Nat. Protoc."},{"issue":"8","key":"2025110608483190900_c31","doi-asserted-by":"publisher","first-page":"2399","DOI":"10.1021\/ja0356176","article-title":"Raman spectroscopic characterization of secondary structure in natively unfolded proteins: \u03b1-synuclein","volume":"126","year":"2004","journal-title":"J. Am. Chem. Soc."},{"issue":"6","key":"2025110608483190900_c32","doi-asserted-by":"publisher","first-page":"e13733","DOI":"10.1111\/srt.13733","article-title":"Advancements in medical research: Exploring Fourier transform infrared (FTIR) spectroscopy for tissue, cell, and hair sample analysis","volume":"30","year":"2024","journal-title":"Skin Res. Technol."},{"issue":"7","key":"2025110608483190900_c33","doi-asserted-by":"publisher","first-page":"1718","DOI":"10.3390\/cancers13071718","article-title":"Unveiling cancer metabolism through spontaneous and coherent Raman spectroscopy and stable isotope probing","volume":"13","year":"2021","journal-title":"Cancers"},{"issue":"15","key":"2025110608483190900_c34","doi-asserted-by":"publisher","first-page":"4967","DOI":"10.1039\/C5AN00342C","article-title":"Exploring the structure and formation mechanism of amyloid fibrils by Raman spectroscopy: A review","volume":"140","year":"2015","journal-title":"Analyst"},{"key":"2025110608483190900_c35","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.sbi.2019.09.001","article-title":"Amyloid structures: Much more than just a cross-\u03b2 fold","volume":"60","year":"2020","journal-title":"Curr. Opin. Struct. Biol."},{"issue":"12","key":"2025110608483190900_c36","doi-asserted-by":"publisher","first-page":"3314","DOI":"10.1110\/ps.041024904","article-title":"FTIR reveals structural differences between native \u03b2-sheet proteins and amyloid fibrils","volume":"13","year":"2004","journal-title":"Protein Sci."},{"issue":"10","key":"2025110608483190900_c37","doi-asserted-by":"publisher","first-page":"2328","DOI":"10.1016\/j.bbamem.2013.04.012","article-title":"ATR-FTIR: A \u201crejuvenated\u201d tool to investigate amyloid proteins","volume":"1828","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"issue":"1","key":"2025110608483190900_c38","doi-asserted-by":"publisher","first-page":"7831","DOI":"10.1038\/ncomms8831","article-title":"Infrared nanospectroscopy characterization of oligomeric and fibrillar aggregates during amyloid formation","volume":"6","year":"2015","journal-title":"Nat. Commun."},{"issue":"1","key":"2025110608483190900_c39","doi-asserted-by":"publisher","first-page":"15902","DOI":"10.1038\/ncomms15902","article-title":"Silk micrococoons for protein stabilisation and molecular encapsulation","volume":"8","year":"2017","journal-title":"Nat. Commun."},{"issue":"1","key":"2025110608483190900_c40","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1038\/nchem.2615","article-title":"An infrared spectroscopy approach to follow \u03b2-sheet formation in peptide amyloid assemblies","volume":"9","year":"2017","journal-title":"Nat. Chem."},{"issue":"17","key":"2025110608483190900_c41","doi-asserted-by":"publisher","first-page":"5918","DOI":"10.3390\/app10175918","article-title":"Peak fitting applied to Fourier transform infrared and Raman spectroscopic analysis of proteins","volume":"10","year":"2020","journal-title":"Appl. Sci."},{"issue":"21","key":"2025110608483190900_c42","doi-asserted-by":"publisher","first-page":"6470","DOI":"10.1021\/jf030649y","article-title":"Comparison of changes in the secondary structure of unheated, heated, and high-pressure-treated \u03b2-lactoglobulin and ovalbumin proteins using Fourier transform Raman spectroscopy and self-deconvolution","volume":"52","year":"2004","journal-title":"J. Agric. Food Chem."},{"issue":"8","key":"2025110608483190900_c43","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1002\/jrs.4335","article-title":"Raman spectroscopy of proteins: A review","volume":"44","year":"2013","journal-title":"J. Raman Spectrosc."},{"issue":"6","key":"2025110608483190900_c44","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1080\/10408390701537385","article-title":"Raman spectroscopy for monitoring protein structure in muscle food systems","volume":"48","year":"2008","journal-title":"Crit. Rev. Food Sci. Nutr."},{"issue":"2","key":"2025110608483190900_c45","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1080\/05704920701829043","article-title":"Fourier transform infrared (FTIR) spectroscopy of biological tissues","volume":"43","year":"2008","journal-title":"Appl. Spectrosc. Rev."},{"issue":"8","key":"2025110608483190900_c46","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1038\/nprot.2014.110","article-title":"Using Fourier transform IR spectroscopy to analyze biological materials","volume":"9","year":"2014","journal-title":"Nat. Protoc."},{"issue":"46","key":"2025110608483190900_c47","doi-asserted-by":"publisher","first-page":"25888","DOI":"10.1039\/C8RA04491K","article-title":"Challenges in application of Raman spectroscopy to biology and materials","volume":"8","year":"2018","journal-title":"RSC Adv."},{"issue":"7","key":"2025110608483190900_c48","doi-asserted-by":"publisher","first-page":"1621","DOI":"10.1021\/acschembio.2c00200","article-title":"Bringing vibrational imaging to chemical biology with molecular probes","volume":"17","year":"2022","journal-title":"ACS Chem. Biol."},{"issue":"8","key":"2025110608483190900_c49","doi-asserted-by":"publisher","first-page":"1494","DOI":"10.1021\/acs.accounts.6b00210","article-title":"Live-cell bioorthogonal chemical imaging: Stimulated Raman scattering microscopy of vibrational probes","volume":"49","year":"2016","journal-title":"Acc. Chem. Res."},{"issue":"34","key":"2025110608483190900_c50","doi-asserted-by":"publisher","first-page":"eadq0294","DOI":"10.1126\/sciadv.adq0294","article-title":"Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe","volume":"10","year":"2024","journal-title":"Sci. Adv."},{"issue":"2","key":"2025110608483190900_c51","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1038\/s41592-023-02137-x","article-title":"Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons","volume":"21","year":"2024","journal-title":"Nat. Methods"},{"issue":"5","key":"2025110608483190900_c52","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1038\/s41551-019-0393-4","article-title":"Spectral tracing of deuterium for imaging glucose metabolism","volume":"3","year":"2019","journal-title":"Nat. Biomed. Eng."},{"issue":"4","key":"2025110608483190900_c53","doi-asserted-by":"publisher","first-page":"2238","DOI":"10.1021\/acs.analchem.2c03683","article-title":"High-throughput antimicrobial susceptibility testing of Escherichia coli by wide-field mid-infrared photothermal imaging of protein synthesis","volume":"95","year":"2023","journal-title":"Anal. Chem."},{"issue":"13","key":"2025110608483190900_c54","doi-asserted-by":"publisher","first-page":"2918","DOI":"10.1021\/acs.jpcb.2c08812","article-title":"Spatiotemporal heterogeneity of de novo lipogenesis in fixed and living single cells","volume":"127","year":"2023","journal-title":"J. Phys. Chem. B"},{"issue":"10","key":"2025110608483190900_c55","doi-asserted-by":"publisher","first-page":"2339","DOI":"10.1016\/j.bbamem.2013.01.014","article-title":"FTIR spectroscopic imaging of protein aggregation in living cells","volume":"1828","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"issue":"4","key":"2025110608483190900_c56","doi-asserted-by":"publisher","first-page":"1672","DOI":"10.1016\/S0006-3495(96)79411-0","article-title":"In situ characterization of beta-amyloid in Alzheimer's diseased tissue by synchrotron Fourier transform infrared microspectroscopy","volume":"71","year":"1996","journal-title":"Biophys. J."},{"issue":"1","key":"2025110608483190900_c57","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.jsb.2005.09.004","article-title":"Synchrotron-based infrared and X-ray imaging shows focalized accumulation of Cu and Zn co-localized with \u03b2-amyloid deposits in Alzheimer's disease","volume":"155","year":"2006","journal-title":"J. Struct. Biol."},{"issue":"4","key":"2025110608483190900_c58","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1016\/j.neuroimage.2009.05.063","article-title":"Amyloid plaques in PSAPP mice bind less metal than plaques in human Alzheimer's disease","volume":"47","year":"2009","journal-title":"Neuroimage"},{"issue":"1","key":"2025110608483190900_c59","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.neuroimage.2011.11.069","article-title":"Biochemical label-free tissue imaging with subcellular-resolution synchrotron FTIR with focal plane array detector","volume":"60","year":"2012","journal-title":"Neuroimage"},{"issue":"14","key":"2025110608483190900_c60","doi-asserted-by":"publisher","first-page":"3991","DOI":"10.1039\/c3an00295k","article-title":"Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer's disease brain","volume":"138","year":"2013","journal-title":"Analyst"},{"issue":"7","key":"2025110608483190900_c61","doi-asserted-by":"publisher","first-page":"3670","DOI":"10.1021\/ac5040659","article-title":"Time-resolved FT-IR microspectroscopy of protein aggregation induced by heat-shock in live cells","volume":"87","year":"2015","journal-title":"Anal. Chem."},{"issue":"20","key":"2025110608483190900_c62","doi-asserted-by":"publisher","first-page":"eabg1559","DOI":"10.1126\/sciadv.abg1559","article-title":"Bond-selective imaging by optically sensing the mid-infrared photothermal effect","volume":"7","year":"2021","journal-title":"Sci. Adv."},{"issue":"43","key":"2025110608483190900_c63","doi-asserted-by":"publisher","first-page":"8597","DOI":"10.1021\/acs.jpcb.2c05827","article-title":"Mid-infrared photothermal microscopy: Principle, instrumentation, and applications","volume":"126","year":"2022","journal-title":"J. Phys. Chem. B"},{"issue":"6","key":"2025110608483190900_c64","doi-asserted-by":"publisher","first-page":"1903004","DOI":"10.1002\/advs.201903004","article-title":"Super-resolution infrared imaging of polymorphic amyloid aggregates directly in neurons","volume":"7","year":"2020","journal-title":"Adv. Sci."},{"issue":"1","key":"2025110608483190900_c65","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1038\/s41377-021-00590-x","article-title":"Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons","volume":"10","year":"2021","journal-title":"Light"},{"issue":"1","key":"2025110608483190900_c66","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1038\/s41377-023-01191-6","article-title":"Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy","volume":"12","year":"2023","journal-title":"Light"},{"issue":"4","key":"2025110608483190900_c67","doi-asserted-by":"publisher","first-page":"2542","DOI":"10.1021\/acs.jmedchem.2c01359","article-title":"Fluorescently guided optical photothermal infrared microspectroscopy for protein-specific bioimaging at subcellular level","volume":"66","year":"2023","journal-title":"J. Med. Chem."},{"issue":"6","key":"2025110608483190900_c68","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1083\/jcb.200112104","article-title":"Huntington toxicity in yeast model depends on polyglutamine aggregation mediated by a prion-like protein Rnq1","volume":"157","year":"2002","journal-title":"J. Cell. Biol."},{"issue":"3","key":"2025110608483190900_c69","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1074\/jbc.M117.812388","article-title":"Structural features of \u03b1-synuclein amyloid fibrils revealed by Raman spectroscopy","volume":"293","year":"2018","journal-title":"J. Biol. Chem."},{"issue":"1","key":"2025110608483190900_c70","doi-asserted-by":"publisher","first-page":"13489","DOI":"10.1038\/srep13489","article-title":"Plaque-associated lipids in Alzheimer's diseased brain tissue visualized by nonlinear microscopy","volume":"5","year":"2015","journal-title":"Sci. Rep."},{"issue":"11","key":"2025110608483190900_c71","doi-asserted-by":"publisher","first-page":"eaat7715","DOI":"10.1126\/sciadv.aat7715","article-title":"Label-free imaging of amyloid plaques in Alzheimer's disease with stimulated Raman scattering microscopy","volume":"4","year":"2018","journal-title":"Sci. Adv."},{"issue":"22","key":"2025110608483190900_c72","doi-asserted-by":"publisher","first-page":"5596","DOI":"10.1002\/anie.201310725","article-title":"Live-cell quantitative imaging of proteome degradation by stimulated Raman scattering","volume":"53","year":"2014","journal-title":"Angew. Chem., Int. Ed."},{"issue":"4","key":"2025110608483190900_c73","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1021\/acscentsci.9b01196","article-title":"Live-cell imaging and quantification of PolyQ aggregates by stimulated Raman scattering of selective deuterium labeling","volume":"6","year":"2020","journal-title":"ACS Cent. Sci."},{"issue":"20","key":"2025110608483190900_c74","doi-asserted-by":"publisher","first-page":"2200315","DOI":"10.1002\/advs.202200315","article-title":"Super-resolution vibrational imaging using expansion stimulated Raman scattering microscopy","volume":"9","year":"2022","journal-title":"Adv. Sci."},{"issue":"33","key":"2025110608483190900_c75","doi-asserted-by":"publisher","first-page":"eadg6061","DOI":"10.1126\/sciadv.adg6061","article-title":"Profiling single cancer cell metabolism via high-content SRS imaging with chemical sparsity","volume":"9","year":"2023","journal-title":"Sci. Adv."},{"issue":"20","key":"2025110608483190900_c76","doi-asserted-by":"publisher","first-page":"2206519","DOI":"10.1002\/advs.202206519","article-title":"Longitudinal single-cell imaging of engineered strains with stimulated Raman scattering to characterize heterogeneity in fatty acid production","volume":"10","year":"2023","journal-title":"Adv. Sci."},{"key":"2025110608483190900_c77","doi-asserted-by":"publisher","first-page":"137363","DOI":"10.1016\/j.snb.2025.137363","article-title":"Bioorthogonal Raman and IR probes for live cell metabolomics: A library","volume":"430","year":"2025","journal-title":"Sens. Actuators, B"},{"issue":"3","key":"2025110608483190900_c78","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1093\/jmicro\/dfaf020","article-title":"Live-cell omics with Raman spectroscopy","volume":"74","year":"2025","journal-title":"Microscopy"},{"issue":"28","key":"2025110608483190900_c79","doi-asserted-by":"publisher","first-page":"18101","DOI":"10.1021\/acsnano.4c04282","article-title":"From genotype to phenotype: Raman spectroscopy and machine learning for label-free single-cell analysis","volume":"18","year":"2024","journal-title":"ACS Nano"},{"key":"2025110608483190900_c80","doi-asserted-by":"publisher","first-page":"e79903","DOI":"10.7554\/eLife.79903","article-title":"Fixation can change the appearance of phase separation in living cells","volume":"11","year":"2022","journal-title":"eLife"},{"issue":"45","key":"2025110608483190900_c81","doi-asserted-by":"publisher","first-page":"24796","DOI":"10.1021\/jacs.3c08854","article-title":"Label-free high-resolution photothermal optical infrared spectroscopy for spatiotemporal chemical analysis in fresh, hydrated living tissues and embryos","volume":"145","year":"2023","journal-title":"J. Am. Chem. Soc."},{"issue":"8","key":"2025110608483190900_c82","doi-asserted-by":"publisher","first-page":"3938","DOI":"10.1021\/acs.analchem.0c04846","article-title":"Analysis of fixed and live single cells using optical photothermal infrared with concomitant Raman spectroscopy","volume":"93","year":"2021","journal-title":"Anal. Chem."},{"key":"2025110608483190900_c83","doi-asserted-by":"publisher","first-page":"e64449","DOI":"10.3791\/64449","article-title":"A flexible chamber for time-lapse live-cell imaging with stimulated Raman scattering microscopy","volume":"186","year":"2022","journal-title":"J. Vis. Exp."},{"issue":"11","key":"2025110608483190900_c84","doi-asserted-by":"publisher","first-page":"2857","DOI":"10.1021\/acs.jpclett.9b00616","article-title":"Cytoplasmic protein imaging with mid-infrared photothermal microscopy: Cellular dynamics of live neurons and oligodendrocytes","volume":"10","year":"2019","journal-title":"J. Phys. Chem. Lett."},{"issue":"1","key":"2025110608483190900_c85","doi-asserted-by":"publisher","first-page":"2995","DOI":"10.1038\/s41467-018-05401-3","article-title":"Optical imaging of metabolic dynamics in animals","volume":"9","year":"2018","journal-title":"Nat. Commun."},{"issue":"1","key":"2025110608483190900_c86","doi-asserted-by":"publisher","first-page":"3052","DOI":"10.1038\/s41467-021-23202-z","article-title":"Microsecond fingerprint stimulated Raman spectroscopic imaging by ultrafast tuning and spatial-spectral learning","volume":"12","year":"2021","journal-title":"Nat. Commun."},{"issue":"24","key":"2025110608483190900_c87","doi-asserted-by":"publisher","first-page":"eadg8814","DOI":"10.1126\/sciadv.adg8814","article-title":"Video-rate mid-infrared photothermal imaging by single-pulse photothermal detection per pixel","volume":"9","year":"2023","journal-title":"Sci. Adv."},{"issue":"1","key":"2025110608483190900_c88","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1038\/s41377-023-01214-2","article-title":"Label-free mid-infrared photothermal live-cell imaging beyond video rate","volume":"12","year":"2023","journal-title":"Light"},{"key":"2025110608483190900_c89","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1016\/j.ijbiomac.2021.03.113","article-title":"The role of amyloid oligomers in neurodegenerative pathologies","volume":"181","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"issue":"1","key":"2025110608483190900_c90","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1038\/s41377-018-0082-1","article-title":"Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130\u2009nm","volume":"7","year":"2018","journal-title":"Light"},{"issue":"43","key":"2025110608483190900_c91","doi-asserted-by":"publisher","first-page":"eadi2181","DOI":"10.1126\/sciadv.adi2181","article-title":"Stimulated Raman photothermal microscopy toward ultrasensitive chemical imaging","volume":"9","year":"2023","journal-title":"Sci. Adv."},{"key":"2025110608483190900_c92","doi-asserted-by":"publisher","first-page":"100195","DOI":"10.1016\/j.newton.2025.100195","article-title":"Self-supervised elimination of non-independent noise in hyperspectral imaging","volume":"1","year":"2025","journal-title":"Newton"},{"issue":"1","key":"2025110608483190900_c93","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/s44303-024-00062-y","article-title":"Metabolic nanoscopy enhanced by experimental and computational approaches","volume":"2","year":"2024","journal-title":"npj Imaging"},{"issue":"5","key":"2025110608483190900_c94","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1038\/s41592-024-02575-1","article-title":"Label-free nanoscopy of cell metabolism by ultrasensitive reweighted visible stimulated Raman scattering","volume":"22","year":"2025","journal-title":"Nat. Methods"},{"issue":"7","key":"2025110608483190900_c95","doi-asserted-by":"publisher","first-page":"3430","DOI":"10.3390\/ijms22073430","article-title":"Amyloid structural changes studied by infrared microspectroscopy in bigenic cellular models of Alzheimer's disease","volume":"22","year":"2021","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"2025110608483190900_c96","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1038\/s41467-023-44675-0","article-title":"Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems","volume":"15","year":"2024","journal-title":"Nat. Commun."},{"key":"2025110608483190900_c97","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1016\/j.cmet.2024.03.014","article-title":"Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK","volume":"36","year":"2024","journal-title":"Cell Metab."},{"issue":"9","key":"2025110608483190900_c98","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1038\/s41566-024-01463-6","article-title":"Millimetre-deep micrometre-resolution vibrational imaging by shortwave infrared photothermal microscopy","volume":"18","year":"2024","journal-title":"Nat. Photonics"},{"key":"2025110608483190900_c99","article-title":"Biomolecular condensates can function as inherent catalysts","year":"2024"},{"issue":"1","key":"2025110608483190900_c100","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1186\/s40478-020-01091-5","article-title":"Label-free vibrational imaging of different A\u03b2 plaque types in Alzheimer's disease reveals sequential events in plaque development","volume":"8","year":"2020","journal-title":"Acta Neuropathol. Commun."},{"issue":"16","key":"2025110608483190900_c101","doi-asserted-by":"publisher","first-page":"10750","DOI":"10.1021\/acs.analchem.9b02286","article-title":"Fingerprinting a living cell by Raman integrated mid-infrared photothermal microscopy","volume":"91","year":"2019","journal-title":"Anal. Chem."},{"key":"2025110608483190900_c102","article-title":"Comprehensive vibrational photothermal microscopy","year":"2024"},{"issue":"29","key":"2025110608483190900_c103","doi-asserted-by":"publisher","first-page":"10809","DOI":"10.1021\/jacs.1c03269","article-title":"Fluorescence-detected mid-infrared photothermal microscopy","volume":"143","year":"2021","journal-title":"J. Am. Chem. Soc."},{"issue":"30","key":"2025110608483190900_c104","doi-asserted-by":"publisher","first-page":"11490","DOI":"10.1021\/jacs.1c03642","article-title":"Fluorescence-detected mid-infrared photothermal microscopy","volume":"143","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"2025110608483190900_c105","doi-asserted-by":"crossref","unstructured":"J.\n            Ao\n          \n          , \u201cIn vivo mapping organellar metabolism by optical-boxcar enhanced fluorescence-detected mid-infrared photothermal microscopy,\u201d arXiv:2504.04305 (2025).","DOI":"10.1117\/12.3042444"},{"key":"2025110608483190900_c106","unstructured":"X.\n            Ge\n          \n          , \u201cFiber laser based stimulated Raman photothermal microscopy with long working distance optics,\u201d arXiv:2504.19875 (2025)."}],"container-title":["Applied Physics Reviews"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.aip.org\/aip\/apr\/article-pdf\/doi\/10.1063\/5.0244025\/20790418\/041312_1_5.0244025.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.aip.org\/aip\/apr\/article-pdf\/doi\/10.1063\/5.0244025\/20790418\/041312_1_5.0244025.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T13:48:40Z","timestamp":1762436920000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.aip.org\/apr\/article\/12\/4\/041312\/3371200\/Feeling-the-vibes-Vibrational-spectroscopic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,6]]},"references-count":106,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12,1]]}},"URL":"https:\/\/doi.org\/10.1063\/5.0244025","relation":{},"ISSN":["1931-9401"],"issn-type":[{"value":"1931-9401","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,12]]},"published":{"date-parts":[[2025,11,6]]},"article-number":"041312"}}