{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T11:30:32Z","timestamp":1772623832005,"version":"3.50.1"},"reference-count":39,"publisher":"AIP Publishing","issue":"2","funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM072744"],"award-info":[{"award-number":["GM072744"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,6,1]]},"abstract":"<jats:p>How forces and mechanics influence and regulate living cells remains elusive. Mechanomemory, the response to a mechanical perturbation that persists after the perturbation is removed, is believed to be a key to understanding the impact of forces and mechanics on cell functions. Recently, our lab has demonstrated the presence of mechanomemory that lasts for \u223c30\u2009min after applying external stress via integrins. Herein, we test the hypothesis that applications of short intermittent episodes of stress exert long-term effects on mechanomemory via the process of mechanotransduction. An Arginine-Glycine-Aspartic acid (RGD)-peptides-coated 4-\u03bcm magnetic bead was bound to the integrin receptors to apply stresses to the surface of a Chinese Hamster Ovary cell. At the same stress magnitude and frequency (15\u2009Pa at 0.3\u2009Hz), multiple cycles of externally applied intermittent 2 or 10\u2009min stresses with 15\u2009min intervals, 10\u2009min stresses with 10\u2009min intervals, or a 30\u2009min stress plus a 30\u2009min load-free interval increased nuclear translocation of YAP (Yes-Associated Protein) and Ctgf gene expression, like that by a 60\u2009min continuous stress, but a 30\u2009min continuous stress did not. Short durations of intermittent stresses increased F-actin in the cytoplasm, which coincided with the elevated YAP translocation. Inhibiting F-actin or actomyosin but not microtubules blocked stress-induced YAP translocation to the nucleus. Cells on soft substrates translocate more YAP than on stiff substrates after external load release. These results highlight the impact of multiple intermittent stresses-induced cytoplasmic mechanomemory on cell biological functions via YAP translocation.<\/jats:p>","DOI":"10.1063\/5.0253046","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T12:55:02Z","timestamp":1744980902000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Mechanomemory after short episodes of intermittent stresses induces YAP translocation via increasing F-actin"],"prefix":"10.1063","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9250-0509","authenticated-orcid":false,"given":"Fazlur","family":"Rashid","sequence":"first","affiliation":[{"name":"The Institute for Mechanobiology, Northeastern University 1 , Boston, Massachusetts 02115,","place":["USA"]},{"name":"Department of Bioengineering, College of Engineering, Northeastern University 2 , Boston, Massachusetts 02115,","place":["USA"]},{"name":"Department of Mechanical Science and Engineering, The Grainger College of Engineering, University of Illinois at Urbana-Champaign 3 , Urbana, Illinois 61801,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0387-8300","authenticated-orcid":false,"given":"Elvis","family":"Njoki","sequence":"additional","affiliation":[{"name":"The Institute for Mechanobiology, Northeastern University 1 , Boston, Massachusetts 02115,","place":["USA"]},{"name":"Department of Bioengineering, College of Engineering, Northeastern University 2 , Boston, Massachusetts 02115,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2478-9464","authenticated-orcid":false,"given":"Sadia Amin","family":"Kabbo","sequence":"additional","affiliation":[{"name":"The Institute for Mechanobiology, Northeastern University 1 , Boston, Massachusetts 02115,","place":["USA"]},{"name":"Department of Bioengineering, College of Engineering, Northeastern University 2 , Boston, Massachusetts 02115,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7531-7385","authenticated-orcid":false,"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"The Institute for Mechanobiology, Northeastern University 1 , Boston, Massachusetts 02115,","place":["USA"]},{"name":"Department of Bioengineering, College of Engineering, Northeastern University 2 , Boston, Massachusetts 02115,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"317","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"2025041808544802900_c1","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.cell.2006.06.044","article-title":"Matrix elasticity directs stem cell lineage specification","volume":"126","year":"2006","journal-title":"Cell"},{"key":"2025041808544802900_c2","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1039\/c2ib00149g","article-title":"The mechanical memory of lung myofibroblasts","volume":"4","year":"2012","journal-title":"Integr. 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