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Micro-objects are placed inside a vertical glass capillary, and two mirrors are positioned behind it at a certain angle, making it possible to have the two reflections of the capillary on the same optical plane. A 3D reconstruction of the trajectories, captured with a single camera, is carried out along the vertical axis with micrometer-scale precision. To investigate the interaction between shear, the role of the gravity field, and motile micro-organisms, we track a model puller-type microalgae, Chlamydomonas reinhardtii, under a Poiseuille flow, using first its natural fluorescence and then bright-field imaging. Understanding how confinement influences motility is crucial, and we show that this 3D tracking setup enables a full description of interactions between a motile organism and a solid border.<\/jats:p>","DOI":"10.1063\/5.0282819","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T13:44:21Z","timestamp":1764942261000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["3D tracking of plankton with single-camera stereoscopy"],"prefix":"10.1063","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8678-3486","authenticated-orcid":false,"given":"J.","family":"Moscatelli","sequence":"first","affiliation":[{"name":"PMMH, CNRS, ESPCI Paris, Universit\u00e9 PSL, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , F-75005 Paris,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Beno\u00eet Gonin","sequence":"additional","affiliation":[{"name":"PMMH, CNRS, ESPCI Paris, Universit\u00e9 PSL, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , F-75005 Paris,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7298-8896","authenticated-orcid":false,"given":"F.","family":"Elias","sequence":"additional","affiliation":[{"name":"PMMH, CNRS, ESPCI Paris, Universit\u00e9 PSL, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , F-75005 Paris,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"317","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"2025120508441862500_c1","volume-title":"The Fluid Dynamics of Cell Motility","year":"2020"},{"key":"2025120508441862500_c2","doi-asserted-by":"publisher","first-page":"20200077","DOI":"10.1098\/rsif.2020.0077","article-title":"Trapping of swimming microalgae in foam","volume":"17","year":"2020","journal-title":"J.\u00a0R. 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