{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T22:19:45Z","timestamp":1773094785431,"version":"3.50.1"},"reference-count":43,"publisher":"AIP Publishing","issue":"10","funder":[{"DOI":"10.13039\/100018703","name":"HORIZON EUROPE European Innovation Council","doi-asserted-by":"publisher","award":["964553"],"award-info":[{"award-number":["964553"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018703","name":"HORIZON EUROPE European Innovation Council","doi-asserted-by":"publisher","award":["101099058"],"award-info":[{"award-number":["101099058"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:p>This work presents the implementation of a new charge detection mass spectrometer (CDMS) design that operates in stand-alone mode, enabled by integration with nanoelectrospray ionization. More specifically, this innovative CDMS consists of a linear charge detection array ion trap spectrometer that combines an eight-tube detector array with conical electrodes. This configuration allows recording data in both transmission mode (linear array) and ion trapping mode (ConeArrayTrap) and measuring the time-of-flight (related to the mass-to-charge ratio) along with the charge of individual ions. Thus, this design supports the high-throughput metrology of viruses at the single-particle level. The devices and geometry of the instrument have been developed based on ion optics simulations. The performance of the current instrument is demonstrated using human norovirus-like particles (hNoVLP) and Adenovirus Ad(5) (hAdV5).<\/jats:p>","DOI":"10.1063\/5.0283803","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:39:35Z","timestamp":1761050375000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Nanoelectrospray ionization coupled to a linear charge detection array ion trap spectrometer for single viral particle analysis"],"prefix":"10.1063","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5587-7182","authenticated-orcid":false,"given":"S.","family":"Maclot","sequence":"first","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7434-8450","authenticated-orcid":false,"given":"T.","family":"Reinert","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7766-9506","authenticated-orcid":false,"given":"L.","family":"Duplantier","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7134-3014","authenticated-orcid":false,"given":"G.","family":"Montagne","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Clavier","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5266-5361","authenticated-orcid":false,"given":"X.","family":"Dagany","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0520-9229","authenticated-orcid":false,"given":"C.","family":"Comby-Zerbino","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kerleroux","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5682-8550","authenticated-orcid":false,"given":"R.","family":"Antoine","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, CNRS 1 , Lyon,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2149-8368","authenticated-orcid":false,"given":"L.","family":"Thiede","sequence":"additional","affiliation":[{"name":"CSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, Leibniz Institute of Virology (LIV), University of L\u00fcbeck 2 , Notkestra\u00dfe 85, 22607 Hamburg, and , Ratzeburger Allee 160, 23562 L\u00fcbeck,","place":["Germany Germany"]},{"name":"Institute of Chemistry and Metabolomics, University of L\u00fcbeck 2 , Notkestra\u00dfe 85, 22607 Hamburg, and , Ratzeburger Allee 160, 23562 L\u00fcbeck,","place":["Germany Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4937-9712","authenticated-orcid":false,"given":"R.","family":"Pogan","sequence":"additional","affiliation":[{"name":"CSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, Leibniz Institute of Virology (LIV), University of L\u00fcbeck 2 , Notkestra\u00dfe 85, 22607 Hamburg, and , Ratzeburger Allee 160, 23562 L\u00fcbeck,","place":["Germany Germany"]},{"name":"Institute of Chemistry and Metabolomics, University of L\u00fcbeck 2 , Notkestra\u00dfe 85, 22607 Hamburg, and , Ratzeburger Allee 160, 23562 L\u00fcbeck,","place":["Germany Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1991-7922","authenticated-orcid":false,"given":"C.","family":"Uetrecht","sequence":"additional","affiliation":[{"name":"CSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, Leibniz Institute of Virology (LIV), University of L\u00fcbeck 2 , Notkestra\u00dfe 85, 22607 Hamburg, and , Ratzeburger Allee 160, 23562 L\u00fcbeck,","place":["Germany Germany"]},{"name":"Institute of Chemistry and Metabolomics, University of L\u00fcbeck 2 , Notkestra\u00dfe 85, 22607 Hamburg, and , Ratzeburger Allee 160, 23562 L\u00fcbeck,","place":["Germany Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1961-075X","authenticated-orcid":false,"given":"A. N.","family":"Kozhinov","sequence":"additional","affiliation":[{"name":"Spectroswiss 3 , 1015 Lausanne,","place":["Switzerland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8993-5739","authenticated-orcid":false,"given":"K. O.","family":"Nagornov","sequence":"additional","affiliation":[{"name":"Spectroswiss 3 , 1015 Lausanne,","place":["Switzerland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-0774","authenticated-orcid":false,"given":"Y. O.","family":"Tsybin","sequence":"additional","affiliation":[{"name":"Spectroswiss 3 , 1015 Lausanne,","place":["Switzerland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2299-9479","authenticated-orcid":false,"given":"D.","family":"Papanastasiou","sequence":"additional","affiliation":[{"name":"Fasmatech Science & Technology, Lefkippos Tech. Park, NCSR Demokritos 4 , 15341 Agia Paraskevi,","place":["Greece"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"317","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"2025102108392699000_c1","doi-asserted-by":"publisher","first-page":"3035","DOI":"10.3892\/MMR.2020.11393","volume":"22","year":"2020","journal-title":"Mol. Med. Rep."},{"key":"2025102108392699000_c2","doi-asserted-by":"publisher","first-page":"e35154","DOI":"10.1016\/j.heliyon.2024.e35154","volume":"10","year":"2024","journal-title":"Heliyon"},{"key":"2025102108392699000_c3","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1002\/mas.21617","volume":"39","year":"2020","journal-title":"Mass Spectrom. Rev."},{"key":"2025102108392699000_c4","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/bs.aivir.2019.07.003","volume":"105","year":"2019","journal-title":"Adv. Virus Res."},{"key":"2025102108392699000_c5","doi-asserted-by":"publisher","first-page":"8596","DOI":"10.1073\/pnas.95.15.8596","volume":"95","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2025102108392699000_c6","doi-asserted-by":"publisher","first-page":"1654","DOI":"10.1039\/c7an00277g","volume":"142","year":"2017","journal-title":"Analyst"},{"key":"2025102108392699000_c7","doi-asserted-by":"publisher","first-page":"e8539","DOI":"10.1002\/rcm.8539","volume":"34","year":"2020","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"2025102108392699000_c8","doi-asserted-by":"publisher","first-page":"4896","DOI":"10.1039\/d1ma00261a","volume":"2","year":"2021","journal-title":"Mater. Adv."},{"key":"2025102108392699000_c9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s43586-023-00203-4","volume":"3","year":"2023","journal-title":"Nat. Rev. Methods Primers"},{"key":"2025102108392699000_c10","doi-asserted-by":"publisher","first-page":"1895","DOI":"10.1021\/ac951128f","volume":"68","year":"1996","journal-title":"Anal. Chem."},{"key":"2025102108392699000_c11","doi-asserted-by":"publisher","first-page":"5951","DOI":"10.1007\/s00216-019-01998-6","volume":"411","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"2025102108392699000_c12","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1002\/1521-3757(20010202)113:3&lt;559::aid-ange559&gt;3.0.co;2-#","volume":"113","year":"2001","journal-title":"Angew. Chem."},{"key":"2025102108392699000_c13","doi-asserted-by":"publisher","first-page":"2386","DOI":"10.1002\/ange.201508995","volume":"128","year":"2016","journal-title":"Angew. Chem."},{"key":"2025102108392699000_c14","doi-asserted-by":"publisher","first-page":"5749","DOI":"10.1021\/jacs.3c08139","volume":"146","year":"2024","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"2025102108392699000_c15","doi-asserted-by":"publisher","first-page":"7415","DOI":"10.1021\/acs.chemrev.1c00377","volume":"122","year":"2021","journal-title":"Chem. Rev."},{"key":"2025102108392699000_c16","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1038\/s41592-024-02207-8","volume":"21","year":"2024","journal-title":"Nat. Methods"},{"key":"2025102108392699000_c17","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1038\/s41592-020-0764-5","volume":"17","year":"2020","journal-title":"Nat. Methods"},{"key":"2025102108392699000_c18","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1002\/rcm.4900","volume":"25","year":"2011","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"2025102108392699000_c19","doi-asserted-by":"publisher","first-page":"114101","DOI":"10.1063\/1.4828668","volume":"84","year":"2013","journal-title":"Rev. Sci. Instrum."},{"key":"2025102108392699000_c20","doi-asserted-by":"publisher","first-page":"043301","DOI":"10.1063\/1.2721408","volume":"78","year":"2007","journal-title":"Rev. Sci. Instrum."},{"key":"2025102108392699000_c21","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1021\/ac102633p","volume":"83","year":"2011","journal-title":"Anal. Chem."},{"key":"2025102108392699000_c22","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1002\/mas.21495","volume":"36","year":"2017","journal-title":"Mass Spectrom. Rev."},{"key":"2025102108392699000_c23","doi-asserted-by":"publisher","first-page":"084104","DOI":"10.1063\/1.3628667","volume":"82","year":"2011","journal-title":"Rev. Sci. Instrum."},{"key":"2025102108392699000_c24","doi-asserted-by":"publisher","first-page":"16554","DOI":"10.1021\/acs.langmuir.3c02526","volume":"39","year":"2023","journal-title":"Langmuir"},{"key":"2025102108392699000_c25","doi-asserted-by":"publisher","first-page":"14060","DOI":"10.1021\/acs.analchem.4c03267","volume":"96","year":"2024","journal-title":"Anal. Chem."},{"key":"2025102108392699000_c26","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.ijms.2012.07.010","volume":"345-347","year":"2013","journal-title":"Int. J. Mass Spectrom."},{"key":"2025102108392699000_c27","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.ijms.2017.01.007","volume":"414","year":"2017","journal-title":"Int. J. Mass Spectrom."},{"key":"2025102108392699000_c28","unstructured":"ARIADNE-Vibe consortium, https:\/\/cordis.europa.eu\/project\/id\/964553\/reporting, 2021, https:\/\/ariadne-vibe.com\/."},{"key":"2025102108392699000_c29","doi-asserted-by":"publisher","first-page":"7192","DOI":"10.1039\/d2cc00200k","volume":"58","year":"2022","journal-title":"Chem. Commun."},{"key":"2025102108392699000_c30","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/s1387-3806(00)00305-5","volume":"200","year":"2000","journal-title":"Int. J. Mass Spectrom."},{"key":"2025102108392699000_c31","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/s0168-583x(00)00415-8","volume":"173","year":"2001","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2025102108392699000_c32","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/s0168-583x(03)00553-6","volume":"205","year":"2003","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2025102108392699000_c33","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.nima.2010.06.008","volume":"621","year":"2010","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. A"},{"key":"2025102108392699000_c34","doi-asserted-by":"publisher","first-page":"77","DOI":"10.2533\/chimia.2025.77","volume":"79","year":"2025","journal-title":"Chimia"},{"key":"2025102108392699000_c35","doi-asserted-by":"publisher","first-page":"1659","DOI":"10.1021\/jasms.5c00074","volume":"36","year":"2025","journal-title":"J.\u00a0Am. Soc. Mass Spectrom."},{"key":"2025102108392699000_c36","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1021\/acs.analchem.1c02439","volume":"94","year":"2022","journal-title":"Anal. Chem."},{"key":"2025102108392699000_c37","doi-asserted-by":"publisher","first-page":"13150","DOI":"10.1021\/acs.analchem.4c01913","volume":"96","year":"2024","journal-title":"Anal. Chem."},{"key":"2025102108392699000_c38","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1021\/jasms.5c00049","volume":"36","year":"2025","journal-title":"J.\u00a0Am. Soc. Mass Spectrom."},{"key":"2025102108392699000_c39","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1021\/jasms.9b00010","volume":"31","year":"2020","journal-title":"J.\u00a0Am. Soc. Mass Spectrom."},{"key":"2025102108392699000_c40","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.nimb.2007.04.298","volume":"262","year":"2007","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2025102108392699000_c41","doi-asserted-by":"publisher","first-page":"7004","DOI":"10.1021\/acs.jpclett.5c01075","volume":"16","year":"2025","journal-title":"J.\u00a0Phys. Chem. Lett."},{"key":"2025102108392699000_c42","doi-asserted-by":"publisher","first-page":"1990","DOI":"10.1021\/jasms.2c00214","volume":"33","year":"2022","journal-title":"J.\u00a0Am. Soc. Mass Spectrom."},{"key":"2025102108392699000_c43","doi-asserted-by":"publisher","first-page":"1482","DOI":"10.3390\/v15071482","volume":"15","year":"2023","journal-title":"Viruses"}],"container-title":["Review of Scientific Instruments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.aip.org\/aip\/rsi\/article-pdf\/doi\/10.1063\/5.0283803\/20767595\/105204_1_5.0283803.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.aip.org\/aip\/rsi\/article-pdf\/doi\/10.1063\/5.0283803\/20767595\/105204_1_5.0283803.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:39:36Z","timestamp":1761050376000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.aip.org\/rsi\/article\/96\/10\/105204\/3368689\/Nanoelectrospray-ionization-coupled-to-a-linear"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,1]]},"references-count":43,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10,1]]}},"URL":"https:\/\/doi.org\/10.1063\/5.0283803","relation":{},"ISSN":["0034-6748","1089-7623"],"issn-type":[{"value":"0034-6748","type":"print"},{"value":"1089-7623","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,10]]},"published":{"date-parts":[[2025,10,1]]},"article-number":"105204"}}