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Recent experiments revealed that, in addition to the coherent signal, action 2D spectra contain incoherent population mixing signals, which are created when two independent linear responses are mixed during the detection time. Disentangling coherent and incoherent contributions is essential for maximizing the informational content and interpretability of action 2D spectroscopy. We use simulations to propose a set of polarizations that selectively suppress the coherent signal and expose spectral signatures of multi-exciton processes occurring during the detection time. We showed that rephasing and non-rephasing spectra contain different incoherent responses. In addition, we showed that for molecular aggregates with efficient exciton\u2013exciton annihilation, the \u27e80\u00b0, 60\u00b0, 120\u00b0, 30\u00b0\u27e9 polarization isolates electronic coherences, while \u27e80\u00b0, 30\u00b0, 90\u00b0, 150\u00b0\u27e9 or \u27e80\u00b0, 45\u00b0, 135\u00b0, 90\u00b0\u27e9 polarizations can be used to study energy transfer dynamics, both of which are otherwise obscured by incoherent signals. Our simulations provide a practical strategy for experimentally isolating incoherent contributions in action-detected 2D spectroscopies.<\/jats:p>","DOI":"10.1063\/5.0334812","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:35:16Z","timestamp":1787567716000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Polarization sequences reveal the incoherent signals generated by multi-exciton photophysics in ultrafast 2D action spectroscopies"],"prefix":"10.1063","volume":"165","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8828-1040","authenticated-orcid":false,"given":"Kennet J.","family":"Rueda Espinosa","sequence":"first","affiliation":[{"name":"Department of Physics and Astronomy, University of Delaware 1 , Newark, Delaware 19716,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2698-4418","authenticated-orcid":false,"given":"Aarti","family":"Sindhu","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Delaware 1 , Newark, Delaware 19716,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7916-7580","authenticated-orcid":false,"given":"Zachary M.","family":"Faitz","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Wisconsin\u2013Madison 2 , Madison, Wisconsin 53706,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7191-9768","authenticated-orcid":false,"given":"Martin T.","family":"Zanni","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Wisconsin\u2013Madison 2 , Madison, Wisconsin 53706,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9422-7203","authenticated-orcid":false,"given":"Alexei A.","family":"Kananenka","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Delaware 1 , Newark, Delaware 19716,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"317","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"2026082406350994600_c1","doi-asserted-by":"publisher","first-page":"214307","DOI":"10.1063\/1.2800560","article-title":"Fluorescence-detected two-dimensional electronic coherence spectroscopy by acousto-optic phase modulation","volume":"127","year":"2007","journal-title":"J.\u00a0Chem. 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