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This study focuses on identifying the main motional modes of water in A\u03b2 fibrils and oligomers formed from the native A\u03b21\u201340 peptide or the toxic, yet naturally occurring, pyroglutamate-3 (PE) post-translational modification. The oligomers and fibrils were hydrated with either D2O or H217O enriched water for quantitative analysis of the dynamics of protein-bound and remote layers, using 2H and 17O solid-state NMR spectroscopy in the 300\u2013170\u00a0K temperature range. Line shape and relaxation measurements, spanning laboratory and rotating frame relaxation, provide complementary assessment of motions in the protein-bound and remote layers, sensing different amplitudes and time scales. The comparison of the water dynamics between all of the A\u03b2 species and a previously characterized globular protein indicates distinct differences. In particular, the general trend is the enhancement of water dynamics in the oligomers compared to fibrils, with the PE oligomer in the lead.<\/jats:p>","DOI":"10.1063\/5.0349500","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:23:29Z","timestamp":1788265409000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Hydration water dynamics in amyloid-\u03b2 aggregates using deuterium and oxygen-17 solid-state NMR spectroscopy"],"prefix":"10.1063","volume":"165","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0747-9355","authenticated-orcid":false,"given":"Bailey","family":"Frazier","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Colorado at Denver 1 , Denver, Colorado 80204,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sommer","family":"Vanden Heuvel","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Colorado 2 , Colorado Springs, Colorado 80918,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1097-6057","authenticated-orcid":false,"given":"Kusum","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Colorado at Denver 1 , Denver, Colorado 80204,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4234-7671","authenticated-orcid":false,"given":"Bangyan","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Colorado at Denver 1 , Denver, Colorado 80204,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A.","family":"Zimmermann","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular & Developmental Biology, University of Colorado at Boulder 3 , Boulder, Colorado 80309,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0075-0410","authenticated-orcid":false,"given":"Riqiang","family":"Fu","sequence":"additional","affiliation":[{"name":"National High Magnetic Field Laboratory 4 , Tallahassee, Florida 32310,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3248-6028","authenticated-orcid":false,"given":"Crystal","family":"Vander Zanden","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Colorado 2 , Colorado Springs, Colorado 80918,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9087-7771","authenticated-orcid":false,"given":"Dmitry","family":"Ostrovsky","sequence":"additional","affiliation":[{"name":"Department of Mathematical and Statistical Sciences, University of Colorado at Denver 5 , Denver, Colorado 80204,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0491-5561","authenticated-orcid":false,"given":"Liliya","family":"Vugmeyster","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Colorado at Denver 1 , Denver, Colorado 80204,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"317","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"2026090108221684500_c1","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1006\/jmbi.1993.1416","article-title":"Contribution of hydration to protein folding thermodynamics. 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