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A lab-on-chip system incorporating a thin-film amorphous silicon (a-Si:H) photodiode microfabricated on a glass substrate with a thin-film amorphous silicon-carbon alloy directly deposited above the photodiode and acting as a fluorescence filter is integrated with a polydimethylsiloxane-based microfluidic network for the direct detection of antibody-antigen molecular recognition reactions using fluorescence. The model immunoassay used consists of primary antibody adsorption to the microchannel walls followed by its recognition by a secondary antibody labeled with a fluorescent quantum-dot tag. The conditions for the flow-through analysis in the microfluidic format were defined and the total assay time was 30 min. Specific molecular recognition was quantitatively detected. The measurements made with the a-Si:H photodiode are consistent with that obtained with a fluorescence microscope and both show a linear dependence on the antibody concentration in the nanomolar-micromolar range.<\/jats:p>","DOI":"10.1063\/1.3553014","type":"journal-article","created":{"date-parts":[[2011,2,16]],"date-time":"2011-02-16T02:45:53Z","timestamp":1297824353000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes"],"prefix":"10.1063","volume":"5","author":[{"given":"A. T.","family":"Pereira","sequence":"first","affiliation":[{"name":"Institute of Nanoscience and Nanotechnology 1 INESC Microsistemas e Nanotecnologias and IN- , R. Alves Redol, 9, 1000-029 Lisbon, Portugal"},{"name":"Instituto Superior T\u00e9cnico 2 IBB-Institute for Biotechnology and Bioengineering, , Av. 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