{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:26:32Z","timestamp":1759775192136,"version":"3.41.2"},"reference-count":19,"publisher":"AIP Publishing","issue":"1","content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,3,1]]},"abstract":"<jats:p>Microfluidics is an emerging technology which allows the miniaturization, integration, and automation of fluid handling processes. Microfluidic systems offer low sample consumption, significantly reduced processing time, and the prospect of massive parallelization. A microfluidic platform was developed for the control of the soluble cellular microenvironment of Saccharomyces cerevisiae cells, which enabled high-throughput monitoring of the controlled expression of alpha-synuclein (aSyn), a protein involved in Parkinson\u2019s disease. Y-shaped structures were fabricated using particle desorption mass spectrometry-based soft-lithography techniques to generate biomolecular gradients along a microchannel. Cell traps integrated along the microchannel allowed the positioning and monitoring of cells in precise locations, where different, well-controlled chemical environments were established. S. cerevisiae cells genetically engineered to encode the fusion protein aSyn-GFP (green fluorescent protein) under the control of GAL1, a galactose inducible promoter, were loaded in the microfluidic structure. A galactose concentration gradient was established in the channel and a time-dependent aSyn-GFP expression was obtained as a function of the positioning of cells along the galactose gradient. Our results demonstrate the applicability of this microfluidic platform to the spatiotemporal control of cellular microenvironment and open a range of possibilities for the study of cellular processes based on single-cell analysis.<\/jats:p>","DOI":"10.1063\/1.3683161","type":"journal-article","created":{"date-parts":[[2012,2,9]],"date-time":"2012-02-09T20:52:33Z","timestamp":1328820753000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["High-throughput study of alpha-synuclein expression in yeast using microfluidics for control of local cellular microenvironment"],"prefix":"10.1063","volume":"6","author":[{"given":"Patr\u00edcia","family":"Rosa","sequence":"first","affiliation":[{"name":"INESC Microsistemas e Nanotecnologias (INESC MN) and IN-Institute of Nanoscience and Nanotechnology 1 , Rua Alves Redol, 9, Lisbon 1000-029, Portugal"}]},{"given":"Sandra","family":"Tenreiro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa Av. Prof. Egas Moniz 2 , 1649-028 Lisboa, Portugal"}]},{"given":"Virginia","family":"Chu","sequence":"additional","affiliation":[{"name":"INESC Microsistemas e Nanotecnologias (INESC MN) and IN-Institute of Nanoscience and Nanotechnology 1 , Rua Alves Redol, 9, Lisbon 1000-029, Portugal"}]},{"given":"Tiago F.","family":"Outeiro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa Av. Prof. Egas Moniz 2 , 1649-028 Lisboa, Portugal"},{"name":"University Medizin Goettingen 3 Department of Neurodegeneration and Restaurative Research, , Goettingen, Germany"}]},{"given":"Jo\u00e3o Pedro","family":"Conde","sequence":"additional","affiliation":[{"name":"INESC Microsistemas e Nanotecnologias (INESC MN) and IN-Institute of Nanoscience and Nanotechnology 1 , Rua Alves Redol, 9, Lisbon 1000-029, Portugal"},{"name":"Instituto Superior T\u00e9cnico 4 Department of Bioengineering, , Av Rovisco Pais 1, Lisbon 1049-001, Portugal"}]}],"member":"317","published-online":{"date-parts":[[2012,2,9]]},"reference":[{"key":"2023080107425599300_c1","doi-asserted-by":"publisher","first-page":"4098","DOI":"10.1021\/ac0354241","volume":"76","year":"2004","journal-title":"Anal. Chem."},{"key":"2023080107425599300_c2","doi-asserted-by":"publisher","first-page":"15","DOI":"10.2116\/analsci.19.15","volume":"19","year":"2003","journal-title":"Anal. 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