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In this work, we present a novel application of microfluidic paper-based analytical devices (\u03bcPADs) to study the behavior of a small model nematode, Caenorhabditis elegans. We describe schemes of \u03bcPAD fabrication on paper and plastic substrates where membranes are created in agarose and Pluronic gel. Methods are demonstrated for loading, visualizing, and transferring single and multiple nematodes. Using an anthelmintic drug, levamisole, we show that chemical testing on C. elegans is easily performed because of the open device structure. A custom program is written to automatically recognize individual worms on the \u03bcPADs and extract locomotion parameters in real-time. The combination of \u03bcPADs and the nematode tracking program provides a relatively low-cost, simple-to-fabricate imaging and screening assay (compared to standard agarose plates or polymeric microfluidic devices) for non-microfluidic, nematode laboratories.<\/jats:p>","DOI":"10.1063\/1.5005829","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T15:59:28Z","timestamp":1507651168000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing"],"prefix":"10.1063","volume":"1","author":[{"given":"Zach","family":"Njus","sequence":"first","affiliation":[{"name":"Department of Electrical and Computer Engineering, Iowa State University 1 , Ames, Iowa 50011, USA"}]},{"given":"Taejoon","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, Iowa State University 1 , Ames, Iowa 50011, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8908-0961","authenticated-orcid":false,"given":"Upender","family":"Kalwa","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, Iowa State University 1 , Ames, Iowa 50011, USA"}]},{"given":"Christopher","family":"Legner","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, Iowa State University 1 , Ames, Iowa 50011, USA"}]},{"given":"Matthew","family":"Weinstein","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, Iowa State University 1 , Ames, Iowa 50011, USA"}]},{"given":"Shawn","family":"Flanigan","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Iowa State University 2 , Ames, 50011, USA"},{"name":"Iowa 2 , Ames, 50011, USA"}]},{"given":"Jenifer","family":"Saldanha","sequence":"additional","affiliation":[{"name":"Department of Genetics, Development and Cell Biology, Iowa State University 3 , Ames, Iowa 50011, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4577-8567","authenticated-orcid":false,"given":"Santosh","family":"Pandey","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, Iowa State University 1 , Ames, Iowa 50011, USA"}]}],"member":"317","published-online":{"date-parts":[[2017,10,9]]},"reference":[{"key":"2023061802560104200_c1","doi-asserted-by":"publisher","first-page":"7951","DOI":"10.1039\/C5AY01724F","volume":"7","year":"2015","journal-title":"Anal. 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