{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:40:16Z","timestamp":1761007216628,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":5388,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>To determine changes in skeletal muscle metabolism after nerve transection and repair and to correlate metabolic changes with functional recovery.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND SETTING<\/jats:title><jats:p>Male Wistar rats were divided into 6 experimental groups plus a control group. The posterior tibial nerve was transected and reapproximated. At varying times after surgery (1, 2, 4, 6, or 8 weeks) animals were sacrificed, the gastrocnemius muscle was harvested, and proton nuclear magnetic resonance (NMR) spectroscopy was performed. Functional recovery was measured using the sciatic function index.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Animals undergoing nerve repair all showed functional recovery whereas the nonrepaired nerve group did not. Concentration of glucose and lactate increased after denervation and then returned toward normal. Choline concentration decreased and then returned toward normal. In animals not undergoing nerve repair, the metabolic abnormalities persisted and showed no sign of recovery over the 8\u2010week observation period.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>1H NMR spectroscopy is a potentially useful tool to study changes in skeletal muscle metabolism after motor nerve injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>SIGNIFICANCE<\/jats:title><jats:p>NMR spectroscopy is rapidly developing into a clinically useful tool. High\u2010field magnets have improved resolution and data acquisition. Basic experiments, such as those described here, will help guide the use of NMR spectroscopy in clinical medicine and will also lead to a better understanding of basic mechanisms of nerve injury and repair.<\/jats:p><\/jats:sec>","DOI":"10.1067\/mhn.2001.120231","type":"journal-article","created":{"date-parts":[[2003,2,4]],"date-time":"2003-02-04T21:49:09Z","timestamp":1044395349000},"page":"617-622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of Skeletal Muscle Denervation and Reinnervation Using Magnetic Resonance Spectroscopy"],"prefix":"10.1002","volume":"125","author":[{"given":"Joseph C.","family":"Dort","sequence":"first","affiliation":[{"name":"Department of Surgery (Otolaryngology) and Department of Clinical Neurosciences University of Calgary Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Surgery (Otolaryngology) and Department of Clinical Neurosciences University of Calgary Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deane D.","family":"McIntyre","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Calgary Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"79","volume-title":"Review of medical physiology","author":"Ganong WF","year":"1997"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1980.00790300040008"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016489409128323"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1492(199802)11:1<1::AID-NBM493>3.0.CO;2-D"},{"key":"e_1_2_5_6_1","first-page":"143","article-title":"Taurine distribution in different cat muscles as visualized by immunohistochemistry: Changes with stimulus rate","volume":"73","author":"Quesada O","year":"1993","journal-title":"Cytobios"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198901000-00024"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(82)90234-5"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000637-199228060-00008"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00101a004"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6565(89)80008-1"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.1993.tb02373.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.1920100111"},{"key":"e_1_2_5_14_1","first-page":"368","article-title":"Biochemical changes in denervated muscle identified by magnetic resonance spectroscopy","volume":"26","author":"Dort JC","year":"1997","journal-title":"J Otolaryngol"},{"key":"e_1_2_5_15_1","first-page":"154","article-title":"Correlation of MRI, clinical, and electroneuronographic findings in acute facial nerve palsy","volume":"17","author":"Brandle P","year":"1996","journal-title":"Am J Otol"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1981.00790370003001"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-6665(20)31122-1"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0266-7681(91)90110-A"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.189.2.8210383"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1227\/00006123-199412000-00010"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)80175-1"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(84)90269-5"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0730-725X(92)90450-E"},{"key":"e_1_2_5_24_1","first-page":"397","article-title":"Observation on incorporation of 3H\u2010taurine in mouse skeletal muscle cells by light and electron microscopic radioautography","volume":"39","author":"Terauchi A","year":"1993","journal-title":"Cell Mol Biol"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1067\/mhn.2001.120231","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1067\/mhn.2001.120231","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1067\/mhn.2001.120231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T12:04:21Z","timestamp":1760961861000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1067\/mhn.2001.120231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1067\/mhn.2001.120231"],"URL":"https:\/\/doi.org\/10.1067\/mhn.2001.120231","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"type":"print","value":"0194-5998"},{"type":"electronic","value":"1097-6817"}],"subject":[],"published":{"date-parts":[[2001,12]]},"assertion":[{"value":"2016-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}