{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T17:00:38Z","timestamp":1773766838453,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":5114,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:sec><jats:title>;OBJECTIVES<\/jats:title><jats:p>Lecithin is a polyunsaturated phosphatidylcholine (PPC), which are high energy functional and structural elements of all biologic membranes. PPC play a rate\u2010limiting role in the activation of numerous membrane\u2010located enzymes, including superoxide dismutase and glutathione, which are important antioxidants protecting cell membranes from damage by reactive oxygen species (ROS). ROS\u2010induced damage to mitochondrial DNA may lead to reduced mitochondrial function in the cochlea and resultant hearing loss.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND SETTING<\/jats:title><jats:p>The effects of lecithin on aging and age\u2010associated hearing loss were studied in rats by measuring hearing sensitivities using auditory brainstem responses (ABR). In addition, mitochondrial function as a measure of aging was assessed by determining mitochondrial membrane potentials using flow cytometry and by amplifying mitochondrial DNA deletions associated with aging. Harlan\u2010Fischer rats aged 18 to 20\u00a0months (n = 14) were divided into 2\u00a0groups. The experimental group was supplemented orally for 6\u00a0months with lecithin, a purified extract of soybean phospholipid (Nutritional Therapeutics, Allendale, NJ).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The data obtained were compared with the control group. ABRs were recorded at 2\u2010month intervals and showed significant preservation of hearing sensitivities in the treated subjects. Flow cytometry revealed significantly higher mitochondrial membrane potentials in the treated subjects, suggesting preserved mitochondrial function. Finally, the common aging mitochondrial DNA deletion (mtDNA<jats:sup>4834<\/jats:sup>) were amplified from brain and cochlear tissue including stria vascularis and auditory nerve. This specific deletion was found significantly less frequent in all tissues in the treated group compared with the controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These experiments support our hypothesis and provide evidence that lecithin may preserve cochlear mitochondrial function and protect hearing loss associated with aging.<\/jats:p><\/jats:sec>","DOI":"10.1067\/mhn.2002.127627","type":"journal-article","created":{"date-parts":[[2003,2,4]],"date-time":"2003-02-04T21:49:09Z","timestamp":1044395349000},"page":"138-144","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["Influence of Lecithin on Mitochondrial DNA and Age\u2010Related Hearing Loss"],"prefix":"10.1002","volume":"127","author":[{"given":"Michael D.","family":"Seidman","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Henry Ford Health System West Bloomfield Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mumtaz J.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Henry Ford Health System West Bloomfield Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen Xue","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Henry Ford Health System West Bloomfield Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne S.","family":"Quirk","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Henry Ford Health System West Bloomfield 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