{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:56:23Z","timestamp":1792108583347,"version":"4.4.0"},"reference-count":53,"publisher":"CSIRO Publishing","issue":"4","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":160,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001117","name":"AW Howard Memorial Trust","doi-asserted-by":"publisher","award":["Research Fellowship"],"award-info":[{"award-number":["Research Fellowship"]}],"id":[{"id":"10.13039\/501100001117","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210103010"],"award-info":[{"award-number":["DP210103010"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Australian Government Research Training Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,11]]},"abstract":"<jats:title\/>\n                  <jats:p>Sorghum (Sorghum bicolor [L.] Moench) is an important forage crop that contains the cyanogenic glucoside dhurrin that releases hydrogen cyanide when tissue is damaged. The acyanogenic (dhurrin-free) sorghum line tcd1 was developed to eliminate the risk of cyanide poisoning from sorghum forage. However, dhurrin may also play a role in nitrogen accumulation and storage. We tested whether dhurrin offers the cyanogenic sorghum line BTx623 a growth advantage relative to tcd1, when nitrogen is limiting and variable. BTx623 and tcd1 were grown under two 42-day nitrogen treatments: high dose, low frequency (\u2018surge\u2019) and low dose, high frequency (\u2018pulse\u2019). BTx623 exhibited no growth advantage or disadvantage compared to tcd1 under either treatment. Young BTx623 plants had high concentrations of dhurrin for defence but rapidly recycled this during nitrogen deficiency under the surge treatment, demonstrating dhurrin\u2019s role in both defence and nitrogen storage. At later stages, surge plants appeared to accumulate influxes of nitrogen in nitrate and amino acids but not dhurrin. There was evidence of gene expression promoting increased biosynthesis and reduced recycling of dhurrin following surge nitrogen applications but not pulse applications. These results deepen our understanding of dhurrin\u2019s role in nitrogen metabolism and demonstrate tcd1\u2019s potential as a safe forage.<\/jats:p>","DOI":"10.1071\/fp24343","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T18:34:27Z","timestamp":1743618867000},"source":"Crossref","is-referenced-by-count":4,"title":["Do cyanogenic glucosides help sorghum manage a fluctuating nitrogen supply?"],"prefix":"10.1071","volume":"52","author":[{"given":"Bethany","family":"English","sequence":"first","affiliation":[{"name":"ASchool of Biological Sciences, Monash University, Clayton, Vic 3800, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia A.","family":"Quinn","sequence":"additional","affiliation":[{"name":"ASchool of Biological Sciences, Monash University, Clayton, Vic 3800, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles R.","family":"Warren","sequence":"additional","affiliation":[{"name":"BSchool of Life and Environmental Sciences, The University of Sydney, Camperdown, NSW 2006, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4756-0411","authenticated-orcid":true,"given":"Roslyn M.","family":"Gleadow","sequence":"additional","affiliation":[{"name":"ASchool of Biological Sciences, Monash University, Clayton, Vic 3800, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2690-6188","authenticated-orcid":true,"given":"Harry","family":"Myrans","sequence":"additional","affiliation":[{"name":"ASchool of Biological Sciences, Monash University, Clayton, Vic 3800, Australia."},{"name":"CInstitute for Climate, Energy and Disaster Solutions, The Australian National University, Canberra, ACT 2600, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"2025111815282115700_R1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.01108","article-title":"Wild sorghum as a promising resource for crop improvement.","volume":"11","author":"Ananda","year":"2020","journal-title":"Frontiers in Plant Science"},{"issue":"2","key":"2025111815282115700_R2","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-022-03831-4","article-title":"Transcript profiles of wild and domesticated sorghum under water-stressed conditions and the differential impact on dhurrin metabolism.","volume":"255","author":"Ananda","year":"2022","journal-title":"Planta"},{"issue":"6","key":"2025111815282115700_R3","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1111\/tpj.13923","article-title":"Glutathione transferases catalyze recycling of auto-toxic cyanogenic glucosides in sorghum.","volume":"94","author":"Bjarnholt","year":"2018","journal-title":"The Plant Journal"},{"issue":"1","key":"2025111815282115700_R4","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1111\/j.1467-7652.2011.00646.x","article-title":"A combined biochemical screen and TILLING approach identifies mutations in Sorghum bicolor L. 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