{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T23:40:35Z","timestamp":1792366835121,"version":"4.4.0"},"reference-count":24,"publisher":"CSIRO Publishing","issue":"10","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,23]]},"abstract":"<jats:p>Mitogen-activated protein kinases (MPKs) are involved in the cold response of plants; however, in tomatoes, their regulation has been studied only at the mRNA level. In this study, a comparative analysis of the transcriptional and translational regulation of the MAPK cascade in tomato (Solanum lycopersicum) under cold stress and hardening was conducted using polysomic profiling with parallel sequencing of total and polysomic RNA fractions. For representatives of all four groups (A\u2013D), as well as the upstream kinases SlMAPKKK, we calculated mRNA differential expression and translational efficiency. Most cascade components were regulated predominantly at the translational level and mRNA levels remained stable. Groups C and D were profiled for the first time under cold conditions, and candidate translational cold regulators were identified.<\/jats:p>","DOI":"10.1071\/fp26167","type":"journal-article","created":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T23:17:07Z","timestamp":1792365427000},"source":"Crossref","is-referenced-by-count":0,"title":["A comparative analysis of the tomato MAPK cascade at the transcriptional and translational levels under low-temperature conditions"],"prefix":"10.1071","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-9496","authenticated-orcid":true,"given":"Alexandra V.","family":"Suhorukova","sequence":"first","affiliation":[{"name":"ATimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan O.","family":"Klychkov","sequence":"additional","affiliation":[{"name":"ATimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2270-065X","authenticated-orcid":true,"given":"Irina V.","family":"Goldenkova-Pavlova","sequence":"additional","affiliation":[{"name":"ATimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8717-5908","authenticated-orcid":true,"given":"Michail S.","family":"Piotrovskii","sequence":"additional","affiliation":[{"name":"ATimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5206-0775","authenticated-orcid":true,"given":"Alexander A.","family":"Tyurin","sequence":"additional","affiliation":[{"name":"ATimiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"2026101819170323600_R1","unstructured":"Anaconda Software Distribution (2020) Anaconda software distribution. Available at https:\/\/docs.anaconda.com [accessed 22 August 2025]"},{"key":"2026101819170323600_R3","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1104\/pp.18.00067","article-title":"The tomato mitogen-activated protein kinase SlMPK1 is as a negative regulator of the high-temperature stress response.","volume":"177","author":"Ding","year":"2018","journal-title":"Plant Physiology"},{"key":"2026101819170323600_R4","unstructured":"FastQC (n.d.) FastQC: a quality control tool for high throughput sequence data. Available at https:\/\/qubeshub.org\/resources\/fastqc [accessed 22 August 2025]"},{"key":"2026101819170323600_R5","doi-asserted-by":"publisher","first-page":"1728","DOI":"10.1104\/pp.103.024414","article-title":"Convergence of signaling pathways induced by systemin, oligosaccharide elicitors, and ultraviolet-B radiation at the level of mitogen-activated protein kinases in Lycopersicon peruvianum suspension-cultured cells.","volume":"132","author":"Holley","year":"2003","journal-title":"Plant Physiology"},{"key":"2026101819170323600_R13","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/S1360-1385(02)02302-6","article-title":"Mitogen-activated protein kinase cascades in plants: a new nomenclature.","volume":"7","author":"Ichimura","year":"2002","journal-title":"Trends in Plant Science"},{"key":"2026101819170323600_R6","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/S1534-5807(02)00205-8","article-title":"NPK1, an MEKK1-like mitogen-activated protein kinase kinase kinase, regulates innate immunity and development in plants.","volume":"3","author":"Jin","year":"2002","journal-title":"Developmental Cell"},{"key":"2026101819170323600_R7","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.gene.2012.01.048","article-title":"Genome-wide analysis of the mitogen-activated protein kinase gene family in Solanum lycopersicum.","volume":"499","author":"Kong","year":"2012","journal-title":"Gene"},{"issue":"114","key":"2026101819170323600_R8","doi-asserted-by":"publisher","DOI":"10.3791\/54231","article-title":"An easy method for plant polysome profiling.","author":"Lecampion","year":"2016","journal-title":"Journal of Visualized Experiments"},{"key":"2026101819170323600_R9","doi-asserted-by":"publisher","DOI":"10.3390\/plants13192715","article-title":"Recent insights into the physio-biochemical and molecular mechanisms of low temperature stress in tomato.","volume":"13","author":"Lee","year":"2024","journal-title":"Plants"},{"key":"2026101819170323600_R11","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1016\/j.devcel.2017.09.025","article-title":"MPK3- and MPK6-mediated ICE1 phosphorylation negatively regulates ICE1 stability and freezing tolerance in Arabidopsis.","volume":"43","author":"Li","year":"2017","journal-title":"Developmental Cell"},{"key":"2026101819170323600_R12","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.932923","article-title":"Harnessing the role of mitogen-activated protein kinases against abiotic stresses in plants.","volume":"14","author":"Majeed","year":"2023","journal-title":"Frontiers in Plant Science"},{"key":"2026101819170323600_R15","unstructured":"Project Jupyter (n.d.) Project jupyter. Available at https:\/\/jupyter.org [accessed 22 August 2025]"},{"key":"2026101819170323600_R16","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.698585","article-title":"Arabidopsis thaliana eIF4E1 and eIF(iso)4E participate in cold response and promote translation of some stress-related mRNAs.","volume":"12","author":"Salazar-D\u00edaz","year":"2021","journal-title":"Frontiers in Plant Science"},{"key":"2026101819170323600_R17","doi-asserted-by":"publisher","first-page":"6003","DOI":"10.1021\/acs.jafc.2c08723","article-title":"SlMPK3 positively regulates the ethylene production of postharvest tomato fruits and is involved in ethylene-mediated cold tolerance.","volume":"71","author":"Shu","year":"2023","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"2026101819170323600_R18","doi-asserted-by":"publisher","first-page":"1887","DOI":"10.1111\/nph.19072","article-title":"SlMPK1- and SlMPK2-mediated SlBBX17 phosphorylation positively regulates CBF-dependent cold tolerance in tomato.","volume":"239","author":"Song","year":"2023","journal-title":"New Phytologist"},{"key":"2026101819170323600_R19","doi-asserted-by":"publisher","DOI":"10.3390\/cells12202445","article-title":"A molecular orchestration of plant translation under abiotic stress.","volume":"12","author":"Suhorukova","year":"2023","journal-title":"Cells"},{"key":"2026101819170323600_R20","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26178604","article-title":"The transcriptional and translational landscape of plant adaptation to low temperatures.","volume":"26","author":"Suhorukova","year":"2025","journal-title":"International Journal of Molecular Sciences"},{"key":"2026101819170323600_R21","doi-asserted-by":"publisher","DOI":"10.1134\/S1021443724610449","article-title":"Diversification of cold adaptation strategies in tomato plants through genetic information implementation.","volume":"72","author":"Suhorukova","year":"2025","journal-title":"Russian Journal of Plant Physiology"},{"key":"2026101819170323600_R22","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.5233953","article-title":"GNU Parallel 20210822 (\u2018Kabul\u2019).","author":"Tange","year":"2021","journal-title":"Zenodo"},{"key":"2026101819170323600_R23","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.molcel.2004.06.023","article-title":"The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis.","volume":"15","author":"Teige","year":"2004","journal-title":"Molecular Cell"},{"key":"2026101819170323600_R24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103032","article-title":"Genome-wide identification of MAPKK and MAPKKK gene families in tomato and transcriptional profiling analysis during development and stress response.","volume":"9","author":"Wu","year":"2014","journal-title":"PLoS ONE"},{"key":"2026101819170323600_R25","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1146\/annurev-phyto-121823-032335","article-title":"Translational regulation of plant stress responses: mechanisms, pathways, and applications in bioengineering.","volume":"63","author":"Xiang","year":"2025","journal-title":"Annual Review of Phytopathology"},{"key":"2026101819170323600_R26","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1007\/s11105-015-0897-3","article-title":"Enhanced tolerance to chilling stress in tomato by overexpression of a mitogen-activated protein kinase, SlMPK7.","volume":"34","author":"Yu","year":"2016","journal-title":"Plant Molecular Biology Reporter"},{"key":"2026101819170323600_R27","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1016\/j.devcel.2017.09.024","article-title":"MAP kinase cascades regulate the cold response by modulating ICE1 protein stability.","volume":"43","author":"Zhao","year":"2017","journal-title":"Developmental Cell"}],"container-title":["Functional Plant Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/fp\/article-pdf\/doi\/10.1071\/FP26167\/1973681\/fp26167.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/fp\/article-pdf\/doi\/10.1071\/FP26167\/1973681\/fp26167.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T23:17:13Z","timestamp":1792365433000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/fp\/article\/53\/10\/FP26167\/278816\/A-comparative-analysis-of-the-tomato-MAPK-cascade"}},"subtitle":[],"editor":[{"given":"Suleyman","family":"Allakhverdiev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,19]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,23]]}},"URL":"https:\/\/doi.org\/10.1071\/fp26167","relation":{},"ISSN":["1445-4408","1445-4416"],"issn-type":[{"value":"1445-4408","type":"print"},{"value":"1445-4416","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,19]]},"article-number":"FP26167"}}