{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:22:16Z","timestamp":1767853336353,"version":"3.49.0"},"reference-count":0,"publisher":"CSIRO Publishing","issue":"8","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":8154,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,1,29]]},"abstract":"<jats:p>Monovulatory brushtail possums ( Trichosurus vulpecula ) were stimulated with exogenous hormones during seasonal anoestrus to overcome ovarian insensitivity and induce ovulation. Seasonal ovarian insensitivity to pregnant mare serum gonadotrophin (PMSG) was overcome by a new porcine follicle-stimulating hormone\/porcine luteinizing hormone (pFSH\/pLH) protocol. This protocol was refined because the original treatment produced oocytes with abnormal morphology. Possums ( n = 12 per group) received eight injections of pFSH of 1.5, 3.0 or 6.0 mg per injection (at 12-h intervals for 4 consecutive days). Ovulation was induced 12 h after the final pFSH injection with a 4-mg injection of pLH. Control animals were treated with the established protocol of a single injection of 15 IU of PMSG, followed 48 h later with an injection of 4 mg of pLH. All females responded to pFSH\/pLH treatment, although optimal stimulation occurred in those receiving 8 \u00d7 3 mg pFSH, with 13\u201314&amp;nbsp;ovulations and recovery of 11\u201312 oocytes per female (8 \u00d7 1.5 mg pFSH: 13 ovulations, 8\u20139 oocytes; 8&amp;nbsp;\u00d7&amp;nbsp;6&amp;nbsp;mg pFSH: 7\u20138 ovulations, 4\u20135 oocytes). In contrast, only seven of 12 females responded to PMSG\/pLH and, of those responding, only 2\u20133 ovulations occurred and only 1\u20132 oocytes per female were recovered. However, around 80% of oocytes recovered after PMSG\/pLH treatment had undergone nuclear maturation (metaphase II\/1st polar body) compared with around 60% of oocytes from pFSH\/pLH-treated animals. In possums killed from 27 to 39 h after pLH treatment, ovulation onset was first observed from 30 h and by 31.5 h, all animals had completed ovulation. Laparoscopic artificial insemination (LAI) was performed on pFSH\/pLH-treated animals to determine whether the oocytes produced were capable of fertilization. Uterine LAI performed 27.5\u201328.5 h after pLH treatment yielded 11\/26 fertilized oocytes (up to 4-cell stage), whereas vaginal LAI performed 13\u201314 h after pLH treatment yielded 21\/53 fertilized oocytes. A proportion of oocytes generated from the refined pFSH\/pLH protocol are thus properly mature and capable of fertilization. Further refinement of the protocol is now needed to improve the yield of fully matured oocytes.<\/jats:p>","DOI":"10.1071\/rd02045","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T17:40:36Z","timestamp":1046972436000},"page":"453-460","source":"Crossref","is-referenced-by-count":10,"title":["Development of a porcine follicle-stimulating hormone and porcine luteinizing hormone induced ovulation protocol in the seasonally anoestrus brushtail possum (\n                    <i>Trichosurus<\/i>\n                    <i>vulpecula<\/i>\n                    )"],"prefix":"10.1071","volume":"14","author":[{"given":"A. M.","family":"Glazier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. C.","family":"Molinia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/14\/8\/453\/297272\/rd02045.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/14\/8\/453\/297272\/rd02045.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T21:55:13Z","timestamp":1761688513000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/14\/8\/453\/47700\/Development-of-a-porcine-follicle-stimulating"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1,29]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,1,29]]}},"URL":"https:\/\/doi.org\/10.1071\/rd02045","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1,29]]}}}