{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:03:39Z","timestamp":1791111819229,"version":"4.1.0"},"reference-count":42,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":7146,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,11,2]]},"abstract":"<jats:p>The present study was conducted to characterise and localise the progesterone receptor (PR) on canine spermatozoa. Using a progesterone\u2013bovine serum albumin\u2013fluorescein isothiocyanate conjugate (PBF) and different monoclonal antibodies (C262 and NCL-PGR against the steroid binding domain and N-terminus of intracellular PR, respectively, and h151 against the hinge domain of the intracellular oestrogen receptor), the PR was identified on the plasma membrane over the acrosomal region. Two proteins (54 kDa and 65 kDa) were detected by recognition of the three monoclonal antibodies using Western blotting. PBF labelling was observed in the majority of cauda epididymal spermatozoa (63 \u00b1 4%), but this labelling was markedly reduced (33 \u00b1 17%) after the addition of canine seminal plasma. Over a 7-h capacitation, the proportion of ejaculated spermatozoa exhibiting PBF labelling (indicating the presence of the PR) increased from 18 \u00b1 10% (onset) to 59 \u00b1 7% by 5 h, where it plateaued. Progesterone (P4) induced the acrosome reaction (AR) in a dose-dependent manner (0, 0.1, 1 and 10 \u00b5g\/mL P4 corresponding to 10 \u00b1 5%, 16 \u00b1 9%, 23 \u00b1 7% and 30 \u00b1 7%). Pre-treatment of capacitated spermatozoa with canine seminal plasma reduced the incidence of the P4-induced AR (12 \u00b1 5%). In addition, treatment with the monoclonal antibodies significantly reduced the incidence of the P4-induced AR (10 \u00b5g\/mL) in capacitated ejaculated spermatozoa from 19 \u00b1 6% to 11 \u00b1 4% (h151, 1 : 10) and 12 \u00b1 6% (C262, 1 : 10), respectively. A typical Scatchard plot revealed one binding with high affinity and low capacity, and another binding with low affinity and high capacity, suggesting at least two different characteristic PR. Taken together, these results demonstrate that P4 induced the AR in a dose-dependent manner via functional transmembranal receptors in the acrosomal region of the canine sperm plasma membrane. The characteristics of this membrane receptor seem similar to those of other mammalian spermatozoa, and it shows structural homology to the intracellular PR.<\/jats:p>","DOI":"10.1071\/rd05074","type":"journal-article","created":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T20:22:36Z","timestamp":1130876556000},"page":"733-741","source":"Crossref","is-referenced-by-count":16,"title":["Characterisation of the progesterone receptor on canine spermatozoa"],"prefix":"10.1071","volume":"17","author":[{"given":"Jui-Te","family":"Wu","sequence":"first","affiliation":[{"name":"ADepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, 250 Ko-Koung Road, Taichung City 402, Taiwan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Shiue","family":"Tsai","sequence":"additional","affiliation":[{"name":"BDepartment of Farm Animal Health, Utrecht University, Yalelaan 7 3584 CL, De Uithof, Utrecht, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang-Lin","family":"Lee","sequence":"additional","affiliation":[{"name":"CDepartment of Animal Science, College of Agriculture and Natural Resources, National Chung-Hsing University, 250 Ko-Koung Road, Taichung City 402, Taiwan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Pang","family":"Cheng","sequence":"additional","affiliation":[{"name":"ADepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, 250 Ko-Koung Road, Taichung City 402, Taiwan."},{"name":"DVeterinary Medical Teaching Hospital, College of Veterinary Medicine, National Chung-Hsing University, 250-1 Ko-Koung Road, Taichung City 402, Taiwan."},{"name":"ECorresponding author. Email: fpcheng@dragon.nchu.edu.tw"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2005,11,2]]},"reference":[{"key":"2025102818182106100_R1"},{"key":"2025102818182106100_R2"},{"key":"2025102818182106100_R3"},{"key":"2025102818182106100_R4"},{"key":"2025102818182106100_R5"},{"key":"2025102818182106100_R6"},{"key":"2025102818182106100_R7"},{"key":"2025102818182106100_R8"},{"key":"2025102818182106100_R9"},{"key":"2025102818182106100_R10"},{"key":"2025102818182106100_R11"},{"key":"2025102818182106100_R12"},{"key":"2025102818182106100_R13"},{"key":"2025102818182106100_R14"},{"key":"2025102818182106100_R15"},{"key":"2025102818182106100_R16"},{"key":"2025102818182106100_R17"},{"key":"2025102818182106100_R18"},{"key":"2025102818182106100_R19"},{"key":"2025102818182106100_R20"},{"key":"2025102818182106100_R21"},{"key":"2025102818182106100_R22"},{"key":"2025102818182106100_R23"},{"key":"2025102818182106100_R24"},{"key":"2025102818182106100_R25"},{"key":"2025102818182106100_R26"},{"key":"2025102818182106100_R27"},{"key":"2025102818182106100_R28"},{"key":"2025102818182106100_R29"},{"key":"2025102818182106100_R30"},{"key":"2025102818182106100_R31"},{"key":"2025102818182106100_R32"},{"key":"2025102818182106100_R33"},{"key":"2025102818182106100_R34"},{"key":"2025102818182106100_R35"},{"key":"2025102818182106100_R36"},{"key":"2025102818182106100_R37"},{"key":"2025102818182106100_R38"},{"key":"2025102818182106100_R39"},{"key":"2025102818182106100_R40"},{"key":"2025102818182106100_R41"},{"key":"2025102818182106100_R42"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/17\/7\/733\/299379\/rd05074.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/17\/7\/733\/299379\/rd05074.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:18:28Z","timestamp":1761689908000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/17\/7\/733\/48109\/Characterisation-of-the-progesterone-receptor-on"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,2]]},"references-count":42,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2005,11,2]]}},"URL":"https:\/\/doi.org\/10.1071\/rd05074","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11,2]]}}}