{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:33:12Z","timestamp":1788478392661,"version":"build-2803163510"},"reference-count":64,"publisher":"CSIRO Publishing","issue":"5","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":6570,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,7,4]]},"abstract":"<jats:p>The endangered black-footed ferret (Mustela nigripes) has benefited from artificial insemination; however, improved sperm cryopreservation protocols are still needed. The present study focused on identifying factors influencing gamete survival during processing before cryopreservation, including: (1) the presence or absence of seminal plasma; (2) temperature (25\u00b0C v. 37\u00b0C); (3) type of medium (Ham\u2019s F10 medium v. TEST yolk buffer [TYB]); (4) cooling rate (slow, rapid and ultra-rapid); and (5) the presence or absence of glycerol. Seminal plasma did not compromise (P &amp;gt; 0.05) sperm motility or acrosomal integrity. Sperm motility traits were maintained longer (P &amp;lt; 0.05) at 25\u00b0C than at 37\u00b0C in Ham\u2019s or TYB, but temperature did not affect (P &amp;gt; 0.05) acrosomal integrity. Overall, TYB maintained optimal (P &amp;lt; 0.05) sperm motility compared with Ham\u2019s medium, but Ham\u2019s medium maintained more (P &amp;lt; 0.05) intact acrosomes than TYB. Slow cooling (0.2\u00b0C min\u20131) was optimal (P &amp;lt; 0.05) compared to rapid cooling (1\u00b0C min\u20131), and ultra-rapid cooling (9\u00b0C min\u20131) was found to be highly detrimental (P &amp;lt; 0.05). Results obtained in TYB with 0% or 4% glycerol were comparable (P &amp;gt; 0.05), indicating that 4% glycerol was non-toxic to ferret sperm; however, glycerol failed to ameliorate the detrimental effects of either rapid or ultra-rapid cooling. The results of the present study demonstrate that the damage observed to black-footed ferret spermatozoa is derived largely from the rate of cooling.<\/jats:p>","DOI":"10.1071\/rd06096","type":"journal-article","created":{"date-parts":[[2008,1,23]],"date-time":"2008-01-23T21:37:39Z","timestamp":1201124259000},"page":"652-663","source":"Crossref","is-referenced-by-count":21,"title":["Slow cooling prevents cold-induced damage to sperm motility and acrosomal integrity in the black-footed ferret (Mustela nigripes)"],"prefix":"10.1071","volume":"19","author":[{"given":"R. M.","family":"Santymire","sequence":"first","affiliation":[{"name":"ADepartment of Reproductive Sciences, Conservation & Research Centre, Smithsonian\u2019s National Zoological Park, Front Royal, VA 22630, USA."},{"name":"DDepartment of Environment Science and Policy, George Mason University, Fairfax, VA 22030, USA."},{"name":"EPresent address: Department of Conservation & Science, Lincoln Park Zoo, Chicago, IL 60614, USA."},{"name":"FCorresponding author. Email: rsantymire@lpzoo.org"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. E.","family":"Marinari","sequence":"additional","affiliation":[{"name":"BUS Fish & Wildlife Service, National Black-Footed Ferret Conservation Centre, Wellington, CO 80549, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. S.","family":"Kreeger","sequence":"additional","affiliation":[{"name":"BUS Fish & Wildlife Service, National Black-Footed Ferret Conservation Centre, Wellington, CO 80549, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. E.","family":"Wildt","sequence":"additional","affiliation":[{"name":"ADepartment of Reproductive Sciences, Conservation & Research Centre, Smithsonian\u2019s National Zoological Park, Front Royal, VA 22630, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. G.","family":"Howard","sequence":"additional","affiliation":[{"name":"CDepartment of Reproductive Sciences, Smithsonian\u2019s National Zoological Park, Washington, DC 20008, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2007,6,1]]},"reference":[{"key":"2025102818350395900_R1"},{"key":"2025102818350395900_R2"},{"key":"2025102818350395900_R3"},{"key":"2025102818350395900_R4"},{"key":"2025102818350395900_R5"},{"key":"2025102818350395900_R6"},{"key":"2025102818350395900_R7"},{"key":"2025102818350395900_R8"},{"key":"2025102818350395900_R9"},{"key":"2025102818350395900_R10"},{"key":"2025102818350395900_R11"},{"key":"2025102818350395900_R12"},{"key":"2025102818350395900_R13"},{"key":"2025102818350395900_R14"},{"key":"2025102818350395900_R15"},{"key":"2025102818350395900_R16"},{"key":"2025102818350395900_R17"},{"key":"2025102818350395900_R18"},{"key":"2025102818350395900_R19"},{"key":"2025102818350395900_R20"},{"key":"2025102818350395900_R21"},{"key":"2025102818350395900_R22"},{"key":"2025102818350395900_R23"},{"key":"2025102818350395900_R24"},{"key":"2025102818350395900_R25"},{"key":"2025102818350395900_R26"},{"key":"2025102818350395900_R27"},{"key":"2025102818350395900_R28"},{"key":"2025102818350395900_R29"},{"key":"2025102818350395900_R30"},{"key":"2025102818350395900_R31"},{"key":"2025102818350395900_R32"},{"key":"2025102818350395900_R33"},{"key":"2025102818350395900_R34"},{"key":"2025102818350395900_R35"},{"key":"2025102818350395900_R36"},{"key":"2025102818350395900_R37"},{"key":"2025102818350395900_R38"},{"key":"2025102818350395900_R39"},{"key":"2025102818350395900_R40"},{"key":"2025102818350395900_R41"},{"key":"2025102818350395900_R42"},{"key":"2025102818350395900_R43"},{"key":"2025102818350395900_R44"},{"key":"2025102818350395900_R45"},{"key":"2025102818350395900_R46"},{"key":"2025102818350395900_R47"},{"key":"2025102818350395900_R48"},{"key":"2025102818350395900_R49"},{"key":"2025102818350395900_R50"},{"key":"2025102818350395900_R51"},{"key":"2025102818350395900_R52"},{"key":"2025102818350395900_R53"},{"key":"2025102818350395900_R54"},{"key":"2025102818350395900_R55"},{"key":"2025102818350395900_R56"},{"key":"2025102818350395900_R57"},{"key":"2025102818350395900_R58"},{"key":"2025102818350395900_R59"},{"key":"2025102818350395900_R60"},{"key":"2025102818350395900_R61"},{"key":"2025102818350395900_R62"},{"key":"2025102818350395900_R63"},{"key":"2025102818350395900_R64"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/19\/5\/652\/300954\/rd06096.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/19\/5\/652\/300954\/rd06096.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:35:08Z","timestamp":1761690908000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/19\/5\/652\/48471\/Slow-cooling-prevents-cold-induced-damage-to-sperm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,1]]},"references-count":64,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,7,4]]}},"URL":"https:\/\/doi.org\/10.1071\/rd06096","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,1]]}}}